Abstract
Aims: The presence of Salmonella
in water constitutes a potential threat to human health, since these pathogenic
microorganisms are involved in a high number of foodborne and waterborne
outbreaks. The aim of the present study was to determine the level of
antibiotic resistance patterns and distribution of Salmonella spp isolates
from coastal waters of Izmir Gulf Turkey. Methods and Results: In this
study, a total of 75 sea water samples obtained from different places not
coastal areas of the Gulf of Izmir and river basins which flow into the called
as inner and middle areas of the gulf and analyzed for presence of Salmonella
spp. by using the ISO 6340:1995 standard. As a result of API 20E test, 4
(5.3%) of the 75 different samples were identified as Salmonella spp.
After the isolation, antibiotic resistance tests were performed according to
the Kirby-Bauer disk diffusion method for total 13 different antibiotics. All
strains showed resistant to at least 5 or more antibiotics and they showed the
most resistance to Erythromycin. PCR method utilized to investigate Class 1
integrons and 9 antibiotic resistance gene regions (Sulfonamide, ampicillin,
aminoglycosides 1-2, trimethoprim, tetracycline, florfenicol, ofloxacin and
gentamicin) in chromosomal and plasmid DNA of these isolates. In this study
isolated strains determined resistance to six of the examined antibiotics
(tetracycline, sulphonamide, aminoglycosides, chloramphenicol, ofloxacin and
gentamicin) and these resistance originate in both chromosomal and plasmid DNA.
Four strains determined the most resistance to aminoglycosides,
chloramphenicol, sulphonamide and ofloxacin groups respectively. Conclusion:
This study shows antimicrobial-resistant Salmonella contamination
status of the aquatic environment in Izmir. These bacteria acquired resistance
genes through different routes, such as human or animal fecal contamination,
genetic mutations, which occurs under presence of antibiotic stresses in
environment, and frequently, bacteria may gained resistance genes from other
bacterial cells in the vicinity.
Keywords: Salmonella spp, resistom, aquatic
environment
References
- Ihsan Yasa, Beril Arkan Demircan, Nazenin Eftekhari Department of biology, Faculty of Sciences, Ege University, Izmir, Turkey
Abstract
References
- Ihsan Yasa, Beril Arkan Demircan, Nazenin Eftekhari Department of biology, Faculty of Sciences, Ege University, Izmir, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 1, 2017 |
| Published in Issue | Year 2017 Issue: Issue 1(2) - 3. INTERNATIONAL BIOCIDAL CONGRESS |