Determination of the most prevalent pool microalgae in order to revise the test microorganisms in the algicidal assays
Abstract
Pool chemicals constitute an important group of biocidal
products and they are expected to effectively inhibit the algal growth in
different types of pools. Two green algae (Scenedesmus quadricauda, Oocystis
parva or O.solitaria) and one cyanobacterium (Phormidium minnesotense)
should be used as test organisms in biocidal (algicidal) activity assays
according to the current regulations in Turkey. The predominant pool microalgae
and Cyanobacteria species can greatly differ among different geographical
areas. Therefore the aim of this study was to determine the most abundant algal
species in different geographical areas of Turkey. A total of 22 pool water
samples were collected from 18 different locations during Spring-Summer period
of 2015. The samples were investigated by microscopy and DGGE (Denaturing Gradient
Gel Electrophoresis) regarding to the presence of algal and cyanobacterial
species. Sixteen of the 22 samples yielded positive results in DGGE analysis
and presence of the algae and cyanobacteria samples were also confirmed by
microscopic examinations. Four different bands were identified and sequenced
from DGGE gel. Two clades were appeared in the phylogenetic tree build with the
sequences of DGGE bands. One includes S.quadricauda and O.parva while
the other clade consist of our sequences and P. minnesotense. According
to our microscopy observations all samples contain unicellular Cyanobacteria
but not any Chlorophyta species. It is clear that the test organisms in
the algicidal assays should be revised including the most abundant local
strains. In the light of our findings we would suggest an addition of
unicellular cyanobacteria besides filamentose cyanobacterium P. minnesotense as
test organism in the current regulations.
Keywords: Pool chemicals, regulations, standard test
organisms, microalgae, DGGE
Keywords: Pharmacy, Students, Biocidal, Awareness
References
- Burçin Karabey, İnci Tüney Kızılkaya, Ataç Uzel, Güven Özdemir Ege University, Faculty of Science, Department of Biology, İzmir, Turkey
Abstract
References
- Burçin Karabey, İnci Tüney Kızılkaya, Ataç Uzel, Güven Özdemir Ege University, Faculty of Science, Department of Biology, İzmir, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | February 1, 2017 |
| Published in Issue | Year 2017 Issue: Issue 1(2) - 3. INTERNATIONAL BIOCIDAL CONGRESS |