Comparison of sterilization times in membrane filter devices with microbial suspensions at different concentrations.

Year 2017, Volume: 2 Issue: 1(3) - Volume 2, Issue 1(3), 328 - 334, 01.06.2017

Özge Gündoğdu Güngör Burcu Aslaner Fatih Sultan Kazdal Ayfer Kına Necla Erdi Gülcan Turan Gani Orhan BÜŞRA Sarım

Abstract

Introduction. Sterilization
of the membrane filter device used in water microbiology analysis is very
important. Aim of the study. To
compare the efficacy of sterilization by burning with alcohol and flaming with
a Bunsen burner. Material and methods. Bacterial
suspensions were prepared at different concentrations and for each concentration,
each analyst studied 10 culture media for each time periods. The contaminated
membrane filter funnels with the prepared samples were sterilized by flaming
for 10, 20, 40 and 60 seconds. Then, the membrane filter was placed and 100 ml
of sterile distilled water was filtered. Membrane filter was placed on
appropriate growth medium and it was incubated. The same procedure was also
carried out by burning with alcohol. Results.
At 10² concentration, bacterial growth was not observed. At 10³
concentration, bacterial growth was observed 6,66% of growth medium at 10
seconds. At 10⁴ concentration, bacterial growth was observed 30% of
growth medium at 10 seconds and 23,3% of growth medium at 20 seconds. At 10⁵,
10⁶ and 10⁷ concentrations, bacterial growth was observed
in different percentages at all the time periods. Bacterial growth was not
observed in sterilization using alcohol burning technique at all the time
periods. Conclusions. Effective
sterilization can be achieved by flame with Bunsen burner with low microbial
concentration suspensions and low time periods. At high concentration
suspensions, membrane filter device can be effectively sterilized by burning
with alcohol.

Key words: sterilization,
sanitation, E.coli, Pseudomonas
aeruginosa, membrane filter device, water quality.

Keywords

Comparison of sterilization times in membrane filter devices with microbial suspensions at different concentrations.

References

• NO*, Büşra Sarım*, Burcu Aslaner*, Fatih Sultan Kazdal*, Ayfer Kına*, Necla Erdi*, Gülcan Turan*, Gani Orhan* *Istanbul Public Health Laboratory Number 3, Istanbul, Turkey.