The effect of lead exposure on thiol disulfide homeostasis and lipid peroxidation in human tissue

Year 2017, Volume 2, Issue 1(4), 24 - 24, 01.11.2017

Ceylan Bal Murat Büyükşekerci Utku Serkant Murat Alışık Meşide Gündüzöz Ömer Hınç Yılmaz Engin Tutkun Özcan Erel

Abstract

Objective:
Several molecular and cellular mechanisms are proposed for lead induced
toxicity yet oxidative stress is the most prominent of them. In this study we
evaluated the redox state in occupationally lead exposed workers by assessing
the dynamic serum thiol-disulfide homeostasis using a novel method.

Methods: The study group consisted 46 male battery workers who admitted to our
clinic for periodic medical examination and the control group was composed of
44 healthy male administrative workers.. We determined the thiol-disulfide
homeostasis parameters of subjects using a novel automated colourimetric assay.
Catalase, ceruloplasmine and myeloperoxidase activities were measured as
oxidative stress indicators and 8-isoprostane level as marker of oxidative
tissue damage.

Findings: Median blood lead and 8-isoprostane levels of exposed group were
significantly higher than control group (8.0(4.1-35.3) μg/dl vs. 1.7(0.30-3.60)
μg/dl, p<0.001 and 4.02(1.30-9.27) ng/ml vs. 3.17(0.65-7.72) ng/ml,
p<0.001, respectively). Among the thiol disulfide homeostasis parameters,
disulfide level and disulfide/native thiol ratio were significantly higher in
exposed group compared to control group. The mean ceruloplasmine, myeloperoxidase
and catalase activities of Pb exposed group and control group did not differ
significantly higher than (94.99±28.45 U/L vs. 88.90±20.66 U/L, p=0.189; and
147.74±60.21U/L vs. 139.91±51.74U/L, p=0.456 and 95(9.50-476.30) (kU/L) vs.
102.80(18.60-307.1) kU/L, p= 0.606, respectively).

Result: We conclude that the thiol-disulfide homeostasis in Pb exposed workers
is disrupted in favor of disulfide which is indicates oxidative stress.
Furthermore Pb induced oxidative stress contributes to elevation of
8-isoprostane.

 

Keywords:
lead
exposure, thiol disulfide homeostasis, lipid peroxidation

Keywords

The effect of lead exposure on thiol disulfide homeostasis and lipid peroxidation in human tissue

References

• Ceylan Bal1, Murat Büyükşekerci2, Utku Serkant3, Murat Alışık1, Meşide Gündüzöz4, Ömer Hınç Yılmaz5, Engin Tutkun6, Özcan Erel1 1Department of Biochemistry, Yıldırım Beyazıt University,Ankara,Turkey 2Department of Pharmacology, Occupational Diseases Hospital, Ankara, Turkey 3Department of Biochemistry, Golbası Hasvak Hospital, Ankara, Turkey 4Department of Family Medicine, Occupational Diseases Hospital, Ankara, Turkey 5Department of Public Health, Yıldırım Beyazıt University,Ankara,Turkey 6Department of Public Health, Bozok University, Yozgat, Turkey