Abstract
Objectives
Cefazolin, a first generation
cephalosporin β-lactam antibiotic,
is commonly used for prophylaxis during cesarean delivery, and in treatment of
pulmonary infections and many surgical procedures. The aim of this work was to
develop a simple, fast and accurate liquid-chromatography-mass spectrometry method
for determination of serum cefazolin.
Methods
Mass spectrometric analyses were
performed using an Shimadzu LC-20-AD (Kyoto, Japan) coupled with a ABSCIEX API
3200 triple quadrupole mass spectrometer (USA) equipped with an electrospray
ion source (ESI) operating in positive mode.
Results
The standard curves for cefazolin
was linear within the range of 0.39-200 µg/ml. Total run time was 5 minutes. Chromatographic
separation was performed on a C18 column
µm, Phenomenex Luna)
formic acid in water and MeOH
at a flow rate of 0.8 ml/min.
Discussion
Serum cefazolin measurement can
be easily performed by LC-MS/MS system to identify the risk of the patients
with therapeutic drug monitoring. This method performs this drug analysis with
high throughput.
Conclusions
Exact determination of cefazolin
provides reliable levels in clinical studies to avoid toxic effects.
Keywords
Antibiotic levels, mass
spectrometry, cefazolin, toxicity.
Acknowledgments: No conflict
of interest
References
- Ali Unlu*, Sedat Abusoglu, Abdullah Sivrikaya
- Department of Biochemistry, SelcukUniversity Faculty of Medicine, Konya, Turkey
Abstract
References
- Ali Unlu*, Sedat Abusoglu, Abdullah Sivrikaya
- Department of Biochemistry, SelcukUniversity Faculty of Medicine, Konya, Turkey
Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | November 1, 2017 |
| Published in Issue | Year 2017 Volume 2, Issue 1(4) |