A comparative study of solvent effect on propolis extraction by ultrasound-assisted extraction

Year 2024, Volume: 6 Issue: 1, 11 - 17, 30.06.2024

Sevgi Kolaylı , Ceren Birinci

https://doi.org/10.51435/turkjac.1445121

Cited By: 2

Abstract

Propolis is a natural bee product obtained from beehives as raw propolis. Propolis extracts obtained from raw propolis with different polarities solvents are used as food supplement agent. The composition of propolis extracts depends on the raw propolis species, extraction methods and extraction solvent. In this study, it is expressed how the phenolic composition of propolis extracts varies depending on the solvent polarity used. The ultrasonic-assisted maceration technique was used to extract an Anatolian raw propolis sample with five different polarity solvents, namely water, methanol, ethanol, isopropanol, and n-butanol using sequential and gradual extractions. The extraction capacity was evaluated by total phenolic substance content (TPC), total flavonoid substance content (TFC), individual phenolic compounds, and antioxidant capacity. The phenolic compositions were analyzed by High Performance Liquid Chromatography (HPLC-PDA) according to the twenty-five phenolic standards. As a result, it was determined 70% ethanolic extract was contained the highest phenolic compounds and antioxidant capacity.

Keywords

Propolis, extraction, solvent, polyphenols, antioxidant

References

• Ç Akçay, Ü Ayata, E Birinci, M Yalçın, S Kolaylı, Some Physical, Biological, Hardness, and Color Properties of Wood Impregnated with Propolis, Forestist, 2022,72:283–93.
• P.M. Cuce, S. Kolaylı, E. Cuce, Enhanced performance figures of solar cookers through latent heat storage and low-cost booster reflectors, International Journal of Low-Carbon Technologies, 2020,15:427–33.
• V. Bankova, B. Trusheva, M. Popova, Propolis extraction methods: a review, Journal of Apicultural Research, 2021, 60(5), 734743.
• A. Kurek-Górecka, Ş. Keskin, O. Bobis, R. Felitti, M. Górecki, M. Otręba, et al, Comparison of the Antioxidant Activity of Propolis Samples from Different Geographical Regions, Plants (Basel), 2022, 11.
• S. Kolaylı, U.Z. Ureyen Esertas, Y. Kara, The Antimicrobial, Anti-Quorum Sensing, and Anti-Biofilm Activities of Ethanolic Propolis Extracts Used as Food Supplements, Biology Bulletin, 2022;49: S21–30.
• M.F. Abo El-Maati, S.A. Mahgoub, S.M. Labib, A.M.A. Al-Gaby, M.F. Ramadan, Phenolic extracts of clove (Syzygium aromaticum) with novel antioxidant and antibacterial activities, European Journal of Integrative Medicine, 2016, 8:4, 494-504.
• S. Kolaylı, C. BİRİNCİ, Y. Kara, A. Ozkok, A.E. Tanuğur Samancı, H. Sahin, et al, A melissopalynological and chemical characterization of Anatolian propolis and an assessment of its antioxidant potential, European Food Research and Technology 2023, 249:1213–33.
• K. Pobiega, K. Kraśniewska, M. Gniewosz, Application of propolis in antimicrobial and antioxidative protection of food quality - A review, Trends Food Sci Technol, 2019,83:53–62.
• M. Cora, C.K. Buruk, S. Ünsal, N. Kaklikkaya, S. Kolayli, Chemical Analysis and in Vitro Antiviral Effects of Northeast Türkiye Propolis Samples against HSV-1, Chem Biodivers, 2023; 20.
• H.I. Guler, G. Tatar, O. Yildiz, A.O. Belduz, S. Kolayli, Investigation of potential inhibitor properties of ethanolic propolis extracts against ACE-II receptors for COVID-19 treatment by molecular docking study, Arch Microbiol, 2021,203:3557.
• A. Odunkıran, M. Şengül, S. Ufuk, Some Characteristics of Honey and Propolis and Their Effects on Covid-19, J. Apit.Nat.,2021,4:2, 129-153.
• R. El Adaouia Taleb, N. Djebli, H. Chenini, H. Sahin, S. Kolayli, In vivo and in vitro anti-diabetic activity of ethanolic propolis extract, J Food Biochem, 2020;44.
• R.D. Oliveira, S.P. Celeiro, C. Barbosa-Matos, A.S. Freitas, S.M. Cardoso, M. Viana-Pereira, Portuguese Propolis Antitumoral Activity in Melanoma Involves ROS Production and Induction of Apoptosis, Molecules, 2022, 27.
• A. Braakhuis, Evidence on the Health Benefits of Supplemental Propolis, Nutrients 2019, 11.
• H. Zaidi, S. Ouchemoukh, N. Amessis-Ouchemoukh, N. Debbache, R. Pacheco, M.L. Serralheiro, et al, Biological properties of phenolic compound extracts in selected Algerian honeys-The inhibition of acetylcholinesterase and α-glucosidase activities, European Journal of Integrative Medicine, 2019,25:77–84.
• A. Huseynova, A. Ali Alakbarlı, Spectrophotometric Analysis of Flavonoid Quantity in Pollen of Amygdalus communis L. and Determination of Biomarkers, J.Apit.Nat., 2023, 6:1, 22–29.
• V.A. Taddeo, F. Epifano, S. Fiorito, S. Genovese, Comparison of different extraction methods and HPLC quantification of prenylated and unprenylated phenylpropanoids in raw Italian propolis, J Pharm Biomed Anal, 2016, 129:219–23.
• N. Pujirahayu, H. Ritonga, Z. Uslinawaty, Properties And Flavonoids Content In Propolis Of Some Extraction Method Of Raw Propolis, International Journal of Pharmacy and Pharmaceutical Sciences, 2014, 6:6, 338-340
• Y. Kara, Z. Can, S. Kolaylı, Applicability of Phenolic Profile Analysis Method Developed with RP-HPLC-PDA to some Bee Product, Brazilian Archives of Biology and Technology, 2022, 65.
• M. Oroian, F. Dranca, F. Ursachi, Comparative evaluation of maceration, microwave and ultrasonic-assisted extraction of phenolic compounds from propolis, J Food Sci Technol 2020, 57:70.
• K.L. Yeo, C.P. Leo, D.J.C. Chan, Ultrasonic Enhancement on Propolis Extraction at Varied pH and Alcohol Content, Journal of Food Process Engineering, 2015, 38:562–70.
• B. Yurt, Effect of Hydrogen-Enriched Solvents on the Extraction of Phytochemicals in Propolis, ACS Omega, 2023, 7:8(15):14264-14270.
• Y.C. Kung, L.S. Chua, M.F. Yam, J. Soo, Alkaline hydrolysis and discrimination of propolis at different pH values using high throughput 2D IR spectroscopy and LC-MS/MS, Bioresour Technol Rep, 2022,18.
• M. Kekeçoğlu, A. Sorucu, Determination of the effect of green extraction solvents on the phenolic acids and flavonoids of propolis, Journal of Research in Veterinary Medicine, 2022, 41:49–54.
• V.L. Singleton, R. Orthofer, R.M. Lamuela-Raventós, Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent, Methods Enzymol, 1999, 299:152–78.
• L.R. Fukumoto, G. Mazza, Assessing Antioxidant and Prooxidant Activities of Phenolic Compounds, J Agric Food Chem, 2000, 48:3597–604.
• I.F. Benzie, J.J. Strain, The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay, Anal Biochem, 1996 ,15:239(1), 70-6.
• M. Cuendet, K. Hostettmann, O. Potterat, W. Dyatmiko, Iridoid Glucosides with Free Radical Scavenging Properties from Fagraea blumei, Helvetica Chimica Acta, 1997, 80, 1144-1152.
• M.G. Miguel, S. Nunes, S.A. Dandlen, A.M. Cavaco, M.D. Antunes, Phenols and antioxidant activity of hydro-alcoholic extracts of propolis from Algarve, South of Portugal, Food and Chemical Toxicology, 2010, 48:3418–23.
• M. Stompor‐gorący, A. Bajek‐bil, M. Machaczka, Chrysin: Perspectives on Contemporary Status and Future Possibilities as Pro-Health Agent, Nutrients, 2021, 13, 2038. M. Balaha, B. De Filippis, A. Cataldi, V. Di Giacomo, CAPE and Neuroprotection: A Review, Biomolecules, 2021,11:1–28.
• C. Omene, M. Kalac, J. Wu, E. Marchi, K. Frenkel, O.A. O’Connor, Propolis and its active component, Caffeic acid phenethyl ester (CAPE), modulate breast cancer therapeutic targets via an epigenetically mediated mechanism of action, J Cancer Sci Ther, 2013, 5:334–42.
• L. Saftić, Ž. Peršurić, E. Fornal, T. Pavlešić, S. Kraljević Pavelić, Targeted and untargeted LC-MS polyphenolic profiling and chemometric analysis of propolis from different regions of Croatia, J Pharm Biomed Anal, 2019, 165:162–72.