Çiçek, Yaprak ve Gövde Dokularında Hypericum montanum L.'nin Fenolik Profilleri ve Antioksidan Kapasitelerinin Karşılaştırmalı Analizi

Year 2025, Volume: 7 Issue: 3, 237 - 249, 30.09.2025

Nursen Aksu Kalmuk

https://doi.org/10.51435/turkjac.1675184

Abstract

Bu çalışma, Hypericum montanum L.'nin çiçek, yaprak ve gövde metanol ekstraktlarında 25 fenolik bileşiğin kantitatif dağılımının değerlendirildiği ve antioksidan testlerle desteklendiği literatürdeki ilk kapsamlı organ-bazlı analizini sunmaktadır. Her bir organ için toplam fenolik ve flavonoid içerikleri belirlenmiş ve antioksidan kapasiteleri DPPH, FRAP ve CUPRAC yöntemleri kullanılarak değerlendirilmiştir. Sonuçlar, bitki organları arasında belirgin kimyasal farklılıklar ortaya koymuştur.
Çiçek dokusunda epikateşin, kateşin, hesperetin ve kuersetin gibi yüksek düzeylerde flavonoid bileşikler tespit edilmiş ve bu durum, CUPRAC ve DPPH testlerinde gözlemlenen güçlü antioksidan aktivite ile iyi bir uyum göstermiştir. Yaprak dokusunda bol miktarda bulunan rozmarinik asit ve 3,4-dihidroksibenzoik asit gibi fenolik asitler, yüksek FRAP değerleri ile tutarlılık göstermiştir. Bu bulgular, bitkinin bazı organlarının doğal antioksidan zenginleştirilmiş formülasyonlara dahil edilme potansiyeline sahip olabileceğini göstermektedir.
Ayrıca, fenolik bileşim verileri, H. montanum’un morfolojik olarak benzer türlerden kemotaksonomik olarak ayrılmasına dair ön bilgiler sağlayabilir. Bu bağlamda, çalışma gelecekteki taksonomik değerlendirmelere katkı sağlayabilecek tamamlayıcı kanıtlar sunmaktadır. Genel olarak, elde edilen bulgular H. montanum’un fitokimyasal ve biyolojik özelliklerine yönelik daha ayrıntılı araştırmalar için bir temel oluşturmaktadır.

Keywords

Hypericum , Antioksidan aktivite , HPLC analizi , Fenolik bileşikler , Flavonoidler , Kemotaksonomi

Comparative analysis of phenolic profiles and antioxidant capacities in the floral, foliar, and stem tissues of Hypericum montanum L.

Abstract

This study presents the first comprehensive organ-based analysis in the literature in which the quantitative distribution of 25 phenolic compounds in the flower, leaf, and stem methanolic extracts of Hypericum montanum L. was evaluated and supported by antioxidant assays. Total phenolic and flavonoid contents were determined for each organ, and antioxidant capacities were assessed using DPPH, FRAP, and CUPRAC methods. The results revealed notable chemical differences among the plant organs.
In the floral tissue, high levels of flavonoids such as epicatechin, catechin, hesperetin, and quercetin were detected, which corresponded well with the strong antioxidant activity observed in the CUPRAC and DPPH assays. Phenolic acids such as rosmarinic acid and 3,4-dihydroxybenzoic acid, which were abundant in the leaf tissue, were consistent with high FRAP values. These findings suggest that certain organs of the plant may have potential for inclusion in natural antioxidant-enriched formulations.
Additionally, the phenolic composition data may offer preliminary insights into the chemotaxonomic differentiation of H. montanum from morphologically similar species. In this respect, the study provides supplementary evidence that could contribute to future taxonomic evaluations. Overall, the findings lay a foundation for more detailed investigations into the phytochemical and biological properties of H. montanum.

Keywords

Hypericum , Antioxidant activity , HPLC analysis , Phenolic compounds , Flavonoids

Ethical Statement

This study does not involve any human participants or animal experiments. The plant material (Hypericum montanum L.) used in the research was collected from its natural habitat in accordance with national regulations and was taxonomically identified by the author. Therefore, ethical approval was not required.

Supporting Institution

This research received no external funding or institutional support.

Thanks

The author would like to express their sincere thanks to Abidin Gümrülçüoğlu for his valuable insights and guidance during the HPLC analysis stage of this study.

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