Germination and Seedling Development Performances of Some Soybean (Glycine max (L.) Merrill) Cultivars Under Salinity Stress

Year 2023, Volume: 4 Issue: 2, 108 - 118, 07.12.2023

Semih Açıkbaş , Mehmet Arif Özyazıcı , Emre Bıçakçı , Gülen Özyazıcı

https://doi.org/10.51801/turkjrfs.1387963

Cited By: 3

Abstract

The aim of the study was to determine the effects of different salt concentrations on the germination and seedling development parameters of some grain and forage soybean (Glycine max (L.) Merrill) cultivars. The study was conducted under controlled conditions in the Field Crops Laboratory of Faculty of Agriculture in Siirt University. The subject of the research consists of different salt concentrations (S0= 0, S1= 50, S2= 100, S3= 150, and S4= 200 mM NaCl) applied to four different soybean cultivars (Adasoy, Nazlıcan, Yeşilsoy, and Anp-2018). The laboratory experiment was set up in Petri dishes according to the randomized complete parcels design with 4 replications. In the study, germination parameters such as germination percentage (%), mean germination time (days), germination index, germination uniformity coefficient, and germination energy, and some seedling parameters such as radicle and stem length (cm), seedling fresh and dry weight (mg) and seedling vigor index were examined. Significant differences have been found among the examined cultivars in terms of all the parameters considered in the evaluations. Adasoy cultivar came to the fore in germination parameters and Adasoy, Anp-2018, and Yeşilsoy cultivars came to the fore in seedling development characteristics. In the study, the effect of salt concentrations on germination and seedling development parameters (except seedling dry weight) was statistically significant (p<0.01). Increasing salt concentrations negatively affected germination and seedling development. The research results indicate that soybean plants are tolerant up to a salt concentration of 50 mM during germination and seedling development stages. It has been concluded that the cultivation of suitable cultivars is important in areas affected by salt stress, and in this regard, the Adasoy cultivar has been identified as a cultivar that can be evaluated under 50-100 mM NaCl conditions.

Keywords

Soybean, salt stress, germination percentage, seedling dry weight, seedling vigor index

References

• Abel, G. H., & MacKenzie, A. J. (1964). Salt tolerance of soybean varieties (Glycine max L. Merrill) during germination and later growth. Crop Science, 4, 157-161.
• Acikbas, S., Ozyazici, M. A., & Bektas, H. (2021). The effect of salinity on root architecture in forage pea (Pisum sativum ssp. arvense L.). Legume Research-An International Journal, 44(4), 407-412.
• Açıkbaş, S., & Özyazıcı, M. A. (2022a). Determination of germination and seedling characteristics of phacelia (Phacelia tanacetifolia Benth.) plant under salt stress. Anadolu 11th International Conference on Applied Sciences, December 29-30, Diyarbakır, Türkiye, p. 996-1004. (In Turkish).
• Açıkbaş, S., & Özyazıcı, M. A. (2022b). Determination of germination and seedling characteristics of bitter vetch (Vicia ervilia L.) plant under salt stress. 5. International Sciences and Innovation Congress, 11-12 November, Ankara, Türkiye, p. 190-197. (In Turkish).
• Açıkgöz, N., & Açıkgöz, N. (2001). Common mistakes in the statistical analyzes of agricultural experiments I. Single factorials. ANADOLU Journal of Aegean Agricultural Research Institute, 11(1), 135-147. (In Turkish).
• Ahmadvand, G., Soleimani, F., Saadatian, B., & Pouya, M. (2012). Effects of seed priming on germination and mergence traits of two soybean cultivars under salinity stress (Glycine max L.). International Research Journal Applied Basic Science, 3, 234-241.
• Ahmed, R., Islam, M. M., Sarker, H. M. U., Hasan, M., Hossain, M. R., Shila, A., & Ahammed, R. (2023). Morphological responses of three contrasting soybean (Glycine max (L.) Merrill) genotypes under different levels of salinity stress in the coastal region of Bangladesh. Journal of Plant Stress Physiology, 9, 18-26.
• Ali, A. S., & Elozeiri, A.A. (2017). Metabolic processes during seed germination. In: J.C. Jimenez-Lopez (Ed.), Seed Biology, Advances in Seed Biology, pp. 141-166.
• Bayraklı, B., Özyazıcı, G., & Özyazıcı, M. A. (2017). The effect of inoculation with various nodosity bacterial cultures collected from samsun province on yield and nitrogen coverage of soybean (Glycine max L.) in the greenhouse and field conditions. Turkish Journal of Agricultural Research, 4(2), 131-142. (In Turkish).
• Bewley, J. D., & Black, M. (1994). Seeds: Physiology of development and germination. Springer New York, NY. Butcher, K., Wick, A.F., DeSutter, T., Chatterjee, A., & Harmon, J. (2016). Soil salinity: A threat to global food security. Agronomy Journal, 108, 2189-2200.
• Ceritoglu, M., Erman, M., & Yildiz, F. (2020). Effect of salinity on germination and some agro-morfological traits in chickpea seedling. ISPEC Journal of Agricultural Science, 4(1), 82-96.
• Ceritoğlu, M., & Erman, M. (2020). Mitigation of salinity stress on chickpea germination by salicylic acid priming. International Journal of Agriculture and Wildlife Science, 6(3), 582-591.
• Chen, X., Jiang, X., Niu, F., Sun, X., Hu, Z., Gao, F., Zhang, H., & Jiang, Q. (2023). Overexpression of lncRNA77580 regulates drought and salinity stress responses in soybean. Plants, 12(1), 181.
• Cramer, G. R., Alberico, G.J., & Smith, F.A. (1994). Salt tolerance is not associated with sodium accumulation of two maize hybrids. Australian Journal of Plant Physiology, 21, 675-692.
• Çakmakcı, T., Şahin, Ü., Kuşlu, Y., Kızıloğlu, F. M., Tüfenkçi, Ş., & Okuroğlu, M. (2016). Management of clean water and wastewater sources in Van agricultural areas. Yuzuncu Yil University Journal of Agricultural Sciences, 26(4), 662-667. (In Turkish).
• Demirkol, G., Yılmaz, N., & Önal Aşcı, Ö. (2019). The effect of salt stress on the germination and seedling growth parameters of a selected forage pea (Pisum sativum ssp. arvense L.) genotype. Kahramanmaraş Sütçü İmam University Journal of Agriculture and Nature, 22(3), 354-359. (In Turkish).
• Ellis, R. A., & Roberts, E. H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9(2), 373-409.
• Essa, T. A. (2002). Effect of salinity stress on growth and nutrient composition of three soybean (Glycine max L. Merrill) cultivars. Journal of Agronomy and Crop Science, 188, 86-93.
• Fagodiya, R. K., Malyan, S. K., Singh, D., Kumar, A., Yadav, R. K., Sharma, P. C., & Pathak, H. (2022). Greenhouse gas emissions from salt-affected soils: Mechanistic understanding of interplay factors and reclamation approaches. Sustainability, 14, 11876.
• Farhoudi, R., & Tafti, M. M. (2011). Effect of salt stress on seedlings growth and ions homeostasis of soybean (Glycine max) Cultivars. Advanced Environmental Biology, 5, 2522-2526.
• Islam, M. M., Ahmed, R., Shila, A., Jhan, P. K., Nuruzzaman, M., & Fahim, M. M. B. (2019). Assessing salt tolerance level of soybean during germination and seedling stage. Journal of Noakhali Science and Technology University, 3(1&2), 8-14.
• Kalsa, K. K., & Abebie, B. (2012). Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp, dasycarpa (Ten.). African Journal of Agricultural Research, 7(21), 3202-3208.
• Kandil, A. A., Sharief, A. E., & Ahmed, Kh. R. (2015). Performance of some soybean Glycine max (L.) Merrill. cultivars under salinity stress to germination characters. International Journal of Agronomy and Agricultural Research, 6(3), 48-56.
• Kaplan, M., & Kara, R. (2014). Effects of physiological characteristics on yield of silage sorghum. Journal of Agricultural Faculty of Gaziosmanpasa University, 31(3), 20-31. (In Turkish).
• Khan, M. A., & Weber, D. J. (2008). Ecophysiology of High Salinity Tolerant Plants (Tasksfor Vegetation Science), 1st ed. Springer, Amsterdam.
• Kingsbury, R. W., & Epstein, E. (1986). Salt sensitivity in wheat. Plant Physiology, 80, 651-654.
• Kondetti, P., Jawali, N., Apte, S. K., & Shitole, M. G. (2012). Salt tolerance in Indian soybean (Glycine max (L.) Merrill) varieties at germination and early seedling growth. Annals of Biological, 3(3), 1489-1498.
• Kumar, A., & Sharma, B. K. (1990). Specific ion effect on germination and seedling growth of wild canary grass (Phalaris minor (L.) Retz). Journal of Advanced Plant Sciences, 3, 321-325.
• Le, L. T. T., Kotula, L., Siddique, K. H. M., & Colmer, T. D. (2021). Na+ and/or Cl− toxicities determine salt sensitivity in soybean (Glycine max (L.) Merr.), mungbean (Vigna radiata (L.) R. Wilczek), cowpea (Vigna unguiculata (L.) Walp.), and common bean (Phaseolus vulgaris L.). International Journal of Molecular Sciences, 22, 1909.
• Li, W., Zhang, H., Zeng, Y., Xiang, L., Lei, Z., Huang, Q., Li, T., Shen, F., & Cheng, Q. (2020). A salt tolerance evaluation method for sunflower (Helianthus annuus L.) at the seed germination stage. Scientific Reports, 10(1), 1-9.
• Lin, X., Liu, B., Weller, J. L., Abe, J., & Kong, F. (2021). Molecular mechanisms for the photoperiodic regulation of flowering in soybean. Journal of Integrative Plant Biology, 63, 981-994.
• Munns R., (2002). Comparative physiology of salt and water stress. Plant Cell Environment, 25, 239-250. Munns, R. & Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, 651-681.
• Ndifon, E. M. (2013). Assessment of salt and drought stresses using soybean (Glycine max (L.) Merrill) seedling as indicator. Advanced in Agriculture, Sciences and Engineering Research, 3, 1102-1111.
• Neves, G. Y. S., Zonetti, P. C., Ferrarese, M. L., Braccini, A. L., & Ferrarese-Filho, O. (2005). Seed germination and seedling growth of soybean (Glycine max (L.) Merill) varieties at germination and early seedling growth under salt stress. Biosocial Journal Uberlandia, 21, 77-83.
• Önal Aşçı, Ö., & Üney, H. (2016). The effect of different salt concentrations on germination and plant growth of hungarian vetch (Vicia pannonica Crantz). Akademik Ziraat Dergisi, 5(1), 29-34. (In Turkish).
• Özyazıcı, G. (2021a). Effects of salicylic acid applications on salt stress in milk thistle (Silybum marianum L.). 3rd International Cukurova Agriculture And Veterinary Congress, 9-10 October, pp. 870-878, Adana, Turkey.
• Özyazıcı, G. (2021b). Determination of germination characteristics of milk thistle (Silybum marianum L.) plant under salt stress. ISPEC 8th International Conference on Agriculture, Animal Sciences and Rural Development, 24-25 December, pp. 256-267, Bingöl, Türkiye.
• Özyazıcı, G., (2022a). Effect of salt stress on germination characteristics of safflower (Carthamus tinctorius L.) genotypes. II. International Conference on Global Practice of Multidisciplinary Scientific Studies, 26-28 July, Batumi, Georgia, pp. 1617-1627. (In Turkish).
• Özyazıcı, G., (2022b). Effect of salt stress on germination traits of basil (Ocimum basilicum L.) plant. 5. International Sciences and Innovation Congress, 11-12 November, Ankara, Türkiye, pp. 257-264. (In Turkish).
• Özyazıcı, G., & Açıkbaş, S. (2021a). Determination of germination characteristics of fenugreek (Trigonella foenum-graecum L.) plant under salt stress. International Hazar Scientific Research Congress-II, 10-12 Nisan, p. 347-353, Bakü, Azerbaycan. (In Turkish).
• Özyazıcı, M. A., & Açıkbaş, S. (2021b). Effects of different salt concentrations on germination and seedling growth of some sweet sorghum [Sorghum bicolor var. saccharatum (L.) Mohlenbr.] cultivars. Turkish Journal of Agricultural Research, 8(2), 133-143.
• Özyazıcı, M. A., & Açıkbaş, S. (2022). The effect of salt stress on the germination and seedling development of woolly-pod vetch Vicia villosa subsp. dasycarpa (Ten.) Cav.. 2. International Black Sea Modern Scientific Research Congress, 21-22 December, p. 556-561, Rize, Türkiye. (In Turkish).
• Papiernik, S. K., Grieve, C. M., Lesch, S. M., & Yates, S. R. (2005). Effects of salinity, imazethapyr, and chlorimuron application on soybean growth and yield. Communications in Soil Science and Plant Analysis, 36(7-8), 951-967.
• Pavli, O. I., Foti, C., Skoufogianni, G., Karastergiou, G., Panagou, A., & Khah, E. M. (2021). Effect of salinity on seed germination and seedling development of soybean genotypes. International Journal of Environmental Sciences & Natural Resources, 27(2), 556210.
• Phang, T.H., Shao, G., & Lam, H. M. (2008). Salt tolerance in soybean. Journal of Integrative Plant Biology, 50(10), 1196-1212.
• Pratap, A., Gupta, S. K., Kumar, J., Mehandi, S., & Pandey, V. R. (2016). Soybean. In: S.K. Gupta (Ed.), Breeding Oilseeds Crops for Sustainable Production, Opportunities and Constraints, Academic Press is an Imprint of Elsevier, pp. 293-315.
• Pratap, A., Gupta, S. K., Kumar, J., & Solanki, R. K. (2012). Soybean. In: S.K. Gupta (Ed.), Technological Innovations in Major World Oil Crops, Vol. I, Breeding, Springer Science, New York, pp. 293-321.
• Rahman, S. U., McCoy, E., Raza, G., Ali, Z., Mansoor, S., & Amin, I. (2023). Improvement of soybean; A way forward transition from genetic engineering to new plant breeding technologies. Molecular Biotechnology, 65(2), 162-180.
• Ramana, G. V., Padhy, S. P., & Chaitanya, K. V. (2012). Differential responses of four soybean (Glycinemax. L) cultivars to salinity stress. Legume Research-An International Journal, 35(3), 185-193.
• Rueden, C. T., Schindelin, J., Hiner, M. C., DeZonia, B. E., Walter, A. E., Arena, E. T., & Eliceiri, K. W. (2017). ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics, 18(1), 529.
• Scott, S. J., Jones, R. A., & Williams, W. A. (1984). Review of data analysis methods for seed germination. Crop Science, 24(6), 1192-1199.
• Shanko, D., Jateni, G., & Debela, A. (2017). Effects of salinity on chickpea (Cicer arietinum L.) landraces during germination stage. Biochemistry and Molecular Biology Journal, 3(2), 9.
• Shannon, M.C., & Grieve, C.M. (1999). Tolerance of vegetable crops to salinity. Scientia Horticulturae, 78, 5-38.
• Soleymani, A., & Shahrajabian, M. H. (2018). Changes in germination and seedling growth of different cultivars of cumin to drought stress. Cercetări Agronomice în Moldova, 1(173), 91-100.
• Thanh Le, L. T., Kotula, L., Colmer, T. D., & Siddique, K. H. M. (2023). Superior salt tolerance in wild soybean (G. soja) is associated with better ion ‘exclusion’ ability from leaves and mesophyll cells than cultivated soybean genotypes (G. max). Environmental and Experimental Botany, 211, 105348.
• TÜİK, 2023. Crop Production Statistics. Turkish Statistical Institute (TÜİK), (https://www.tuik.gov.tr/), (Accessed Date: 23.08.2023). (In Turkish).
• Valliyodan, B., Ye, H., Song, L., Murphy, M., Shannon, J. G., & Nguyen, H. T. (2017). Genetic diversity and genomic strategies for improving drought and waterlogging tolerance in soybeans. Journal of Experimental Botany, 68(8), 1835-1849.
• Wang, D., & Shannon, M. C. (1999). Emergence and seedling growth of soybean cultivars and maturity groups under salinity. Plant and Soil, 214, 117-124.
• Wang, Y. R., Yu, L., Nan, Z. B., & Liu, Y. L. (2004). Vigor tests used to rank seed lot quality and predict field emergence in four forage species. Crop Sciences, 44(2), 535-541.
• Xu, X. Y., Fanun, R., Zheng, R., Li, C., & Yui, D. Y. (2011). Proteomic analysis of seed germination under salt stress in soybean (Glycine max (L.) Merrill). Journal Zhejiang University Science B, 12, 507-517.
• Zapata, P. J., Serrano, M., Pretel, M. T., Amoros, A., & Botella, M. A. (2004). Polyamines and ethylene changes during germination of different plant species under salinity. Plant Science, 167(4), 781-788.
• Zar, J. H. (1996). Biostatistical analysis. 3rd ed. Prentice Hall, New Jersey, USA.
• Zhang, W., Liao, X., Cui, Y., Ma, W., Zhang, X., Du, H., Ma, Y., Ning, L., Wang, H., Huang, F., Yang, H., Kan, G., & Yu, D. (2019). A cation diffusion facilitator, GmCDF1, negatively regulates salt tolerance in soybean. PLOS Genetics, 15, e1007798.
• Zhang, Y., He, J., Wang, Y., Xing, G., Zhao, J., Li, Y., Yang, S., Palmer, R. G., Zhao, T., & Gai, J. (2015). Establishment of a 100-seed weight quantitative trait locus–allele matrix of the germplasm population for optimal recombination design in soybean breeding programmes. Journal of Experimental Botany, 66(20), 6311-6325.
• Zhou, X., Tian, Y., Qu, Z., Wang, J., Han, D., & Dong, S. (2023). Comparing the salt tolerance of different spring soybean varieties at the germination stage. Plants, 12, 2789.