ISOLATION AND CHARACTERIZATION OF WATER SOLUBLE FRACTION OF PROPOLIS AND ITS ANTIBACTERIAL POTENTIAL ON BACTERIA CAUSING CONJUNCTIVITIS

Year 2024, , 167 - 176, 18.11.2024

Ömer Ersin Muz , Şaban Keskin , Yakup Kara , Şengül Alpay Karaoğlu , Merve Keskin

https://doi.org/10.31467/uluaricilik.1465376

Abstract

Propolis is a bee product with a variety of biological activities. Although chemical composition of propolis differs by the location but all propolis types possesses antimicrobial activity. The usage of propolis for apitherapeutic purposes has increased recently. But its ethanol solubility limits its usage in certain areas like ophthalmology. Main objective of this study is to isolate water soluble components of propolis and determination of its antimicrobial activity against two bacteria causing conjunctivitis namely Neisseria gonorrhoeae and Haemophilus influenzae. Isolation of water soluble fraction of propolis was carried out in two steps by using pectin-propolis micro beads. Isolated water soluble fraction and crude extract was examined by thin layer chromatography and HPLC analyses. Three main spots were screened on TLC plate after isolation. These spots could be explained by the presence of different class of compounds in the isolate. HPLC analyses showed that water soluble fraction contained phenolic acids, their esters and flavonoids like p-OH benzoic acid, t-cinnamic acid, pinocembrin and caffeic acid phenethyl ester. Low antimicrobial activity was achieved against tested microorganisms for the fraction. It can be concluded that isolation of water soluble fraction of the propolis extract could be a solution for its usage in restricted areas.

Keywords

Propolis, Water soluble fraction, Conjunctivitis, Antimicrobial activity

Propolisten Suda Çözünür Bir Fraksiyonun İzolasyonu, Karakterizasyonu ve Bakteriyel Konjonktivite Karşı Potansiyel Etkinliğinin Belirlenmesi

Abstract

Propolis farklı biyolojik aktivitelere sahip doğal bir arı ürünüdür. Propolisin kimyasal bileşimi lokasyona göre farklılık gösterse de tüm propolis türleri antimikrobiyal aktiviteye sahiptir. Propolisin apiterapötik amaçlarla kullanımı son zamanlarda artmıştır. Ancak propolisin etanolde çözünürlüğü, oftalmoloji gibi bazı alanlarda kullanımını sınırlamaktadır. Bu çalışmanın temel amacı propolisin suda çözünen bileşenlerini izole etmek ve izolatların konjonktivite neden olan iki bakteriye karşı antimikrobiyal aktivitesini tespit etmektir. Propolisin suda çözünür fraksiyonunun izolasyonu pektin-propolis mikro boncukları kullanılarak iki aşamada gerçekleştirildi. İzole edilmiş suda çözünür fraksiyon, ince tabaka kromatografisi ile incelendi ve ham ekstraktla karşılaştırıldı. İzolasyondan sonra TLC plakasında üç ana nokta tarandı. Bu lekeler izolatta fenolik asitler, flavonoidler ve kafeik asit esterleri gibi farklı sınıftaki bileşiklerin varlığıyla açıklanabilir. Suda çözünebilen fraksiyonun fenolik asitler, bunların esterleri ve p-OH benzoik asit, t-sinnamik asit, pinosembrin ve kafeik asit fenetil ester gibi flavonoidleri içerdiği belirlendi. Fraksiyon için test edilen mikroorganizmalara karşı düşük antimikrobiyal aktivite elde edildi. Propolis ekstraktının suda çözünen kısmının izolasyonunun kısıtlı alanlarda kullanımı için bir çözüm olabileceği sonucuna varılabilir.

Keywords

propolis, sulu fraksiyon, konjiktivit, antimikrobiyal aktivite

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