Türkiye Propolis Su Ekstraktlarının Antimikrobiyal ve Antiviral Etkilerinin Araştırılması: İn Vitro Çalışma

Year 2024, , 252 - 266, 18.11.2024

Merve Cora , Ülkü Zeynep Üreyen Esertaş , Zeliha Aydın Kasap , Neşe Kaklıkkaya , Sevgi Kolaylı

https://doi.org/10.31467/uluaricilik.1520324

Abstract

Yüzyıllardır tedavi edici ajan olarak kullanılan ve doğal bir arı ürünü olan propolis, mevcut ilaçların yetersiz kalması sonucu yeni bileşenler arayan bilim adamlarının ilgisini çeken doğal kaynaklardan biridir. Bu çalışmada Türkiye’nin kuzeydoğusundan (Ardahan, Rize, Trabzon) toplanan üç farklı propolis örneğinden su ile hazırlanan propolis ekstraktlarının antimikrobiyal ve antiviral aktivitesi araştırıldı. Ekstraktların toplam fenolik içeriği (TPC) ve toplam flavonoid içeriği (TFC) ölçüldü. TPC ve TFC'nin sırasıyla 5.87±0.36 ila 20.47±1.46 mg GAE g-1 ve 0.48±0.04 ila 2.10±0.22 mg QUE g-1 arasında değiştiği belirlendi. Ekstraktların 14 mikroorganizmaya (Bacillus cereus ATCC14579, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 27853, Acinetobacter haemolyticus ATCC 19002, Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Enterobacter aerogenes ATCC 13048, Mycobacterium smegmatis ATCC 607, Klebsiella pneumoniae ATCC 13883, Chromobacterium violaceum ATCC 12472, Candida parapsilosis ATCC 22019 ve Candida albicans ATCC 10231) karşı antimikrobiyal aktivitesi ve Pseudomonas aeruginosa biyofilmine etkinliği, ayrıca anti-quorum sensing ve anti-swarming aktiviteleri araştırıldı. Ekstraktların Herpes simpleks virüs tip 1 (HSV-1)’e karşı antiviral aktivitesi, MTT ve qRT-PCR yöntemleriyle test edildi. Çalışmada kullanılan propolis su ekstraktlarının antimikrobiyal, anti-swarming, anti-quorum sensing ve anti-viral aktivite göstermediği, bununla birlikte ekstraktların güçlü anti-biyofilm aktivitesine sahip olduğu tespit edildi. Sonuçlar, propolis su ekstraktlarının bakteri biyofilmi tedavisinde değerlendirilebileceğini göstermektedir.

Keywords

Antimikrobiyal, Biyofilm, Fenolik, Propolis, RT-PCR

Project Number

TDK-2016-5602

INVESTIGATION OF ANTIMICROBIAL AND ANTIVIRAL EFFECTS OF TÜRKIYE PROPOLIS WATER EXTRACTS: AN IN VITRO STUDY

Abstract

Propolis is a natural bee product used as a therapeutic agent for centuries. Propolis extracts are natural resources that attract the attention of scientists looking for new components due to the insufficiency of existing drugs. In current study, antiviral and antimicrobial activity of propolis water extracts prepared from three different raw propolis samples collected from Northeast of Türkiye (Ardahan, Rize, and Trabzon) were investigated. The total flavonoid contents (TFC) and total phenolic content (TPC) of the extracts were measured. It was determined that TPC and TFC ranged from 5.87±0.36 to 20.47±1.46 mg GAE g-1, and 0.48±0.04 to 2.10±0.22 mg QUE g-1, respectively. The antimicrobial activity of the extracts against 14 microorganisms (Bacillus cereus ATCC14579, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa ATCC 27853, Acinetobacter haemolyticus ATCC 19002, Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Enterobacter aerogenes ATCC 13048, Mycobacterium smegmatis ATCC 607, Klebsiella pneumoniae ATCC 13883, Chromobacterium violaceum ATCC 12472, Candida parapsilosis ATCC 22019, and Candida albicans ATCC 10231) and their effect against the Pseudomonas aeruginosa biofilm were investigated. Additionally, anti-quorum sensing and anti-swarming activities of the extracts were tested. The antiviral activity of the extracts was examined against Herpes simplex virus type 1 (HSV-1) by MTT and qRT-PCR methods. The water extracts of propolis samples did not show antimicrobial, anti-swarming, anti-quorum sensing, and anti-viral activities. However, extracts were found to have strong anti-biofilm activities. The results show that aquatic propolis extracts can be evaluated in the treatment of biofilms.

Keywords

Antimicrobial, Biofilm, Phenolic, Propolis, RT-PCR

Ethical Statement

This study was approved by the local Ethics Committee of Karadeniz Technical University School of Medicine (Protocol/plan code of the research: 2017-166).

Supporting Institution

Karadeniz Technical University, Scientific Research Projects Unit

Project Number

TDK-2016-5602

Thanks

I would like to thank Prof. Dr. Murat Erturk for providing HSV-1, and Prof. Dr. Aykut Ozdarendeli for providing Vero cell lines.

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