İran’ın Batısında Yayılan Cüce Balarısı (Apis florea Fabricius) Popülasyonlarında RAPD Analizine Dayalı Genetik Varyasyon

Year 2014, , 4 - 14, 29.05.2014

Ayça Özkan Koca

https://doi.org/10.31467/uluaricilik.376711

Abstract

Bu çalışmada RAPD-PCR analizini kullanarak İran’daki üç eyaletten örneklenen Apis florea popülasyonları
içindeki ve arasındaki genetik ilişkiyi ve popülasyon farklılaşmasını araştırdık. İran’ın İlam,
Khuzestan ve Bushehr eyaletlerine ait dokuz lokasyondan toplam 158 A. florea kolonisi çalışma için
değerlendirildi. Test edilen 25 primerden 10 tanesi toplamda 115 fragment ile belirlendi. Bu çalışmadaki
popülasyonlar yüksek düzeyde genetik varyasyon göstermiştir (H=0.21). Genetik uzaklığa göre,
genetik olarak en uzak popülasyonlar Soush ve Dezful; ve en yakın popülasyonlar Sarollah ve Musiyan’dır.
Nei genetik uzaklığına bağlı olarak oluşturulan ağaçta üç eyaletteki A. floreapopülasyonları
sadece tek bir büyük küme olarak gruplanmıştır. Uzaklık ile izolasyon testi, coğrafik ve genetik uzaklık
arasında anlamlı bir ilişki göstermemiştir. Ancak her eyaletteki popülasyonlar, diğer eyaletlerdeki
popülasyonlardan daha çok benzerlik göstermiştir.

Keywords

Apis florea, genetik varyasyon, RAPD-PCR, İran

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