İran’ın Batısında Yayılan Cüce Balarısı (Apis florea Fabricius) Popülasyonlarında RAPD Analizine Dayalı Genetik Varyasyon

Year 2014, Volume: 14 Issue: 1, 4 - 14, 29.05.2014

Ayça Özkan Koca

https://doi.org/10.31467/uluaricilik.376711

Abstract

Bu çalışmada RAPD-PCR analizini kullanarak İran’daki üç eyaletten örneklenen Apis florea popülasyonları
içindeki ve arasındaki genetik ilişkiyi ve popülasyon farklılaşmasını araştırdık. İran’ın İlam,
Khuzestan ve Bushehr eyaletlerine ait dokuz lokasyondan toplam 158 A. florea kolonisi çalışma için
değerlendirildi. Test edilen 25 primerden 10 tanesi toplamda 115 fragment ile belirlendi. Bu çalışmadaki
popülasyonlar yüksek düzeyde genetik varyasyon göstermiştir (H=0.21). Genetik uzaklığa göre,
genetik olarak en uzak popülasyonlar Soush ve Dezful; ve en yakın popülasyonlar Sarollah ve Musiyan’dır.
Nei genetik uzaklığına bağlı olarak oluşturulan ağaçta üç eyaletteki A. floreapopülasyonları
sadece tek bir büyük küme olarak gruplanmıştır. Uzaklık ile izolasyon testi, coğrafik ve genetik uzaklık
arasında anlamlı bir ilişki göstermemiştir. Ancak her eyaletteki popülasyonlar, diğer eyaletlerdeki
popülasyonlardan daha çok benzerlik göstermiştir.

Keywords

Apis florea, genetik varyasyon, RAPD-PCR, İran

References

• Bohonak, A.J. 2002. IBD (Isolation By Distance): a program for analyses of isolation by distance. Journal of Heredity, 93: 153-154.
• Chaiyawong, T., Deowanish, S., Wongsiri, S., Sylvester, H.A., Rinderer, T.E., De Guzman, L. 2004.Multivariate morphometric study of Apis florea in Thailand.Journal of Apicultural Research, 43: 123-127.
• Doyle, J.J., Doyle, J.L. 1991. Isolation of plant DNA from fresh tissue.Focus, 12(1): 13-15.
• Haddad, N., De Miranda, J.R., Bataena, A. 2008. Discovery of Apis florea in Aqaba, Jordan. Journal of Apicultural Research, 47: 173-174.
• Haddad, N., Fuchs, S., Hepburn, H.R., Radloff, S.E. 2009. Apis florea in Jordan: source of the founder population. Apidologie, 40: 508-512.
• Hashemi, M. 2004. The complete guide to bee keeping.2nd edn.730 p.
• Hepburn, H.R., Radloff, S.E., Otis, G.W., Fuchs, S., Verma, L.R., Ken, T., Chaiyawong, T., Tahmasebi, G., Ebadi, R., Wongsiri, S. 2005. Apis florea: morphometrics, classification and biogeography. Apidologie, 36: 359-376.
• Hunt, G.J., Page, R.E. 1992. Patterns of inheritance with RAPD molecular markers reveal novel types of polymorphism in the honey bee. Theoretical and Applied Genetics,85: 15-20.
• Hunt, G.J., Page, R.E. 1995. Linkage map of the honey bee, Apis mellifera, based on RAPD markers. Genetics, 139: 1371-1382.
• Jain, S.K., Neekhra, B., Pandey, D., Jain, K. 2010. RAPD marker system in insect study: A review. IndianJournal of Biotechnology, 9: 7-12.
• Kandemir, İ., Moradi, M.G., Özden, B., Özkan, A. 2009. Wing geometry as a tool for studying the population structure of dwarf honey bees (Apis florea Fabricius 1876) in Iran.Journal of Apicultural Research, 48: 238-246.
• Kimura, M., Crow, J.M. 1978.Effect of overall phenotypic selection on genetic change at individual loci.Proceedings of the National Academy of Sciences of the United States of America, 75: 6168-617.
• Langella, O. 1999. Populations, version 1.2.30. A population genetic software. CNRS UPR9034, available from: http://bioinformatics.org/~tryphon/populations/.
• Lewontin, R.C. 1972. The apportionment of human diversity.Evolutionary Biology, 6: 381-398.
• Luke, A.H., Oldroyd, B.P., Wattanachaiyingcharoen, W., Barron, A.B., Nanork, P., Wongsiri, S. 2001.Worker policing in the bee Apis florea.Behavioral Ecology and Sociobiology, 49:509–513.
• Lynch, M., Milligan, B.G. 1994. Analysis of population genetic structure with RAPD markers.Molecular Ecology, 3: 91-99.
• McDermott, J.M., McDonald, B.A. 1993. Gene flow in plant photosystems.AnnualReview of Phytopathology, 31: 353-373.
• Mogga, G.B., Ruttner, F. 1988. Apis florea in Africa; source of the founder population.Bee World, 69: 100-103.
• Moradi, M.G., Kandemir, I. 2005. Observations on Apis florea “the Dwarf Honey Bee” in Iran.American Bee Journal, 145: 498-502.
• Mossadegh, M.S. 1993. New geographical distribution line of Apis florea in Iran. In: Corner, L.J., Rinderer, T., Slyvester, H.A., Wongsiri, S. (eds.) Asian apiculture. Wicwas Press, Cheshire, Connecticut, USA, pp. 64-66.
• Nei, M. 1972. Genetic distance between populations.The American Naturalist, 106: 283-292.
• Nei, M. 1987. Molecular evolutionary genetics. Columbia University Press, New York.
• Otis, G.W. 1996. Distribution of recently recognized species of honeybees (Hymenoptera: Apidae: Apis) in Asia. Journalof the Kansas EntomologicalSociety, 69:311-333.
• Özkan, A., Gharleko, M.M., Özden, B., Kandemir, İ. 2009. Multivariate morphometric study of Apis florea distributed in Iran.Turkish Journal of Zoology, 33: 93-102.
• Palmer, K.A., Oldroyd, B.P. 2001. Mating frequency in Apis florea revisited (Hymenoptera, Apidae). Insectes Sociaux, 48: 40–43.
• Rohlf, F.J. 2005. NTSYS-PC Numerical taxonomy and multivariate analysis system, version 2.20. Exeter Software, New York.
• Ruttner, F., Mossadegh, M.S., Kauhausen-Keller, D. 1995.Distribution and variation of size of Apis florea F in Iran.Apidologie, 26: 477-486.
• Suazo, A., McTiernan, R., Hall, H.G. 1998. Differences between African and European honey bees (Apis mellifera L.) in Random Amplified Polymorphic DNA (RAPD).Journal of Heredity, 89(1): 32-36.
• Tahmasebi, G., Ebadi, R., Tajabadi, N., Akhondi, N., Faraji, S. 2002. The effect of geographical and climatological conditions on the morphometrical variation and separation of Iranian small honeybee (Apis florea F.) populations.Journal of Science and Technology of Agriculture and Natural Resources, 6: 176-185.
• Tunca, R.I., Bilgen, G., Kence, M., Türkmut, L. 2004. Genetic analysis of honeybees of Van region in Turkey with RAPD method. First European Conference on Apidology, Udine, Italy, 19-23 September 2004, Proceeding book page 45.
• Tunca, R.I., Kence, M. 2011. Genetic diversity of honey bee (Apis mellifera L.: Hymenoptera: Apidae) populations in Turkey revealed by RAPD markers. African Journalof AgriculturalResearch, 6: 6217-6225.
• Welsh, J., McClelland, M. 1990. Fingerprinting genomics using PCR with arbitrary primers.Nucleic Acids Research, 18: 7213-7218.
• Wongsiri, S., Lekprayoon, C., Thapa, R., Thirakupt, K., Rinderer, T.E., Sylvester, H.A., Oldrody, B.P., Booncham, U. 1996.Comparative biology of Apis andreniformis and Apis florea in Thailand.Bee World, 77: 23-35.
• Wright, S. 1969. Evolution and genetics of populations: The theory of gene frequencies. Chicago: Univ. Chicago Press.
• Yeh, F.C., Yang, R.C., Boyle, T. 2000. Popgene, version 1.32, Microsoft windows–based freeware for population genetic analysis.Center for International Forestry Research, University of Alberta, Edmonton, Alberta, Canada.