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Antimicrobial Analysis of Propolis Samples From Different Regions in Turkey

Year 2003, Volume: 03 Issue: 3, 16 - 18, 26.08.2003

Abstract

Propolis (bee glue) is a resinous material which honey bees make by collecting material (A. mellifera L.) from plants and mixing it with beeswax. Propolis is used to reduce the entry of air into the hive and to enforce the thin sides of the frame. Propolis is used in folk medicine, apitherapy, cosmetics and the pharmaceutical industry for varies purposes because of reported antibacterial, antiviral and antifungal properties and anti-inflammatory properties. In this research the antibacterial activity of propolis collected from the different regions of Turkey is investigated. Antibacterial activity was assayed using S. aureus and E. coli. All of the propolis samples exhibited activity against S. aureus while the antibacterial activity against E. coli was found to be weaker. Key Words: Propolis, antibacterial activity, S. aureus, E. coli,

References

  • Amoros, M., Sımoes, C. M. O., Girre, L., Sauvager, F., Cormıer, M. 1994. Comparision of the anti Herpes simplex virus activities of propolis and 3-methyl-butyl –2-enyl caffeate. Journal of natural products, 57: 644- 647.
  • Bankova, V., Boudourova-Krasteva, G., Sforcin, J. M., Frete, X., Kujumgiev, A., Maimoni-Rodella, R., Popov, S. 1999. Phytochemical evidence for the plant origin of Brazilian propolis from Sao Paulo State. Z. Naturforsch. 54c:401-405.
  • Bankova, V., Marcucci, M. C. 2000. Standardization of propolis: present status and perspectives. Bee World 81(14):182-188.
  • Banskota, A. H., Tezuka, Y., Prasain, J. K., Matsushige, K., Saiki, I., Kadota, S. 1998. Chemical constituents of Brazilian propolis and their cytotoxic activities. J. Nat. Prod, 61:896-900
  • Bonvehi, J. S., Coll, F. V., 1994. Phenolic composition of propolis from China and from South America. Z. Naturforsch. 49c:712-718.
  • Bosio, K, Avanzini, C., D’avolio, A. ; Ozino, O., Savoia, D. 2000. In vitro activity of propolis against Streptococcus pyogenes. Letters in Applied Microbiology, 31:174- 177.
  • Dimov, V., Ivanovska, N., Manolova, N., Bankova, V., Nikolov, N., Popov, S., 1991. Immunomodulatory action of propolis. Influence on anti-infectious protection and macrophage function. Apidologie, 22:155-162.
  • Garcia-Viguera, C. 1992. Composition of propolis from two different Spanish region. Z. Naturforsch., 47c: 634-637.
  • Ghısalbertı, E., 1979. Propolis: A Rewiev. Bee World 60:59- 84.
  • Hayashi, K., Komura, S., Isajı, N., Ohıshı, N., Yagı, K., 1999. İsolation of antioksidative compounds from Brazilian propolis: 3, 4-dihydroxy-5-prenylcinnamic acid, a novel potent antioxidant. Chem. Pharm. Bull., 47 (11): 1521-1524.
  • Ikeno, K., Ikeno, T., Miyazawa, C. 1991. Effects of propolis on dental caries in rats. Carries Res., 25: 347-351.
  • Jorgensen, J. H., Ferraro, M. J., Craig, W. A., Doern, G., Finegold, S. M., FungTom, C., J., Hansen, S. L., Hindler, J., Reller, L. B., Swenson, J. M. ; Tenover, F. C., Testa, R. T., Wikler, M. A., 1997. Guidance on Review Criteria for Assesment of antimicrobial susceptibility devices. National Commitee for Clinical Laboratory Standards, Mrch 8, 2000. (www. nccls. org), 26p.
  • Kujumgiev, A., Bankova, V., Ignatova, A., Popov, S. 1999. Antibacterial activity of propolis, some of its components and their analogs. Pharmazie 48: 785-786.
  • Marcucci, M. C., Ferreres, F., Garcia-Viguera C., Bankova V. S., De Castro, S. L., Dantas, A. P., Valente, P. H. M., Paulino, N. 2001. Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of ethnopharmacology 74:105-112.
  • Metzner, J., Bekemeıer, H., Paintz, M., Schneidewind, E. 1979. Zur antimikrobiellen Wirksamkeit von propolis und propolisinhaltsstoffen. Pharmazie, 34: 97-102.
  • Mıtamura, T., Matsuno, T., Sakamoto, S., Maemura, M., Kudo, H., Suzuki, S., Kuwa, K., Yoshımura, S., Sassa, S., Nakayama, T., Nagasawa, H. 1996. Effects of a new clerodane diterpenoid isolated from propolis on chemically induced skin tumors in mice. Anticancer Research, 16: 2699-2672.
  • Ota, C. Unterkircher, C., Fantinato, V., Shimuzu, M. T. 2001. Antifungal activity of propolis on different species of Candida. Mycoses 44: 375-378.
  • Öztürk, F., Kurt, E., İnan, U. U., Emiroğlu, L., İlker, S. S. 1999. The effects of acetylcholine and propolis extract on corneal epithelial wound healing in rats. Cornea, 18(4): 466-471.
  • Sforcin, J. M., Fernandes Jr, A., Lopes, C. A. M., Bankova, V., Funari, S. R. C., 2000. Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology, 73:243-249.

Türkiye’nin Farklı Coğrafik Bölgelerinden Toplanan Propolis Örneklerinin Antimikrobiyel Analizi

Year 2003, Volume: 03 Issue: 3, 16 - 18, 26.08.2003

Abstract

Propolis (arı yapışkanı) bal arılarının (Apis mellifera L. ) bitkilerin canlı kısımlarından topladıkları, mumla karıştırıp yuvalarının yapımı ve adaptasyonunda kullandıkları yapışkan koyu renkli reçinemsi bir materyaldir. Propolis savunmayı kolaylaştırmak için yada kovan girişinden hava girişini azaltmak için, çerçevelerin ince kenarlarını güçlendirmek ve tamir etmek için, çatlak ve yarıkları kapatmak için kullanılmaktadır. Bal arıları propolisin mekanik ve biyolojik özelliklerinden çok iyi faydalanmaktadır; örneğin kovan içinde öldürülen ancak kovan dışına taşınamayan düşmanları mumyalayarak çürümesini önlenmektedir. Propolis, antibakteriyel, antifungal ve antiviral özellikleriyle birlikte antiinflammatör, antiülser, lokal anestetik, antitümör, immünostimülatör gibi biyolojik aktiviteleri nedeniyle popüler bir ilaç olarak halk tıbbında, apiterapide, biokozmetikte ve ilaç sanayiinde çeşitli amaçlarla kullanılmıştır. Bu araştırmada Türkiye’nin farklı coğrafik bölgelerine ait illerden (Bursa, Đzmir, Kayseri, Sivas, Yozgat, Erzurum, Hatay, Artvin) toplanan propolis örneklerinin S. aureus ve E. coli bakterilerine karşı anitibakteriyel aktivitelerini incelenmiştir. Đncelenen propolis örneklerinin tümü S. aureus’a karşı önemli aktivite gösterirken, E. coli’ye karşı gözlenen antibakteriyel aktivite daha zayıf bulunmuştur.

References

  • Amoros, M., Sımoes, C. M. O., Girre, L., Sauvager, F., Cormıer, M. 1994. Comparision of the anti Herpes simplex virus activities of propolis and 3-methyl-butyl –2-enyl caffeate. Journal of natural products, 57: 644- 647.
  • Bankova, V., Boudourova-Krasteva, G., Sforcin, J. M., Frete, X., Kujumgiev, A., Maimoni-Rodella, R., Popov, S. 1999. Phytochemical evidence for the plant origin of Brazilian propolis from Sao Paulo State. Z. Naturforsch. 54c:401-405.
  • Bankova, V., Marcucci, M. C. 2000. Standardization of propolis: present status and perspectives. Bee World 81(14):182-188.
  • Banskota, A. H., Tezuka, Y., Prasain, J. K., Matsushige, K., Saiki, I., Kadota, S. 1998. Chemical constituents of Brazilian propolis and their cytotoxic activities. J. Nat. Prod, 61:896-900
  • Bonvehi, J. S., Coll, F. V., 1994. Phenolic composition of propolis from China and from South America. Z. Naturforsch. 49c:712-718.
  • Bosio, K, Avanzini, C., D’avolio, A. ; Ozino, O., Savoia, D. 2000. In vitro activity of propolis against Streptococcus pyogenes. Letters in Applied Microbiology, 31:174- 177.
  • Dimov, V., Ivanovska, N., Manolova, N., Bankova, V., Nikolov, N., Popov, S., 1991. Immunomodulatory action of propolis. Influence on anti-infectious protection and macrophage function. Apidologie, 22:155-162.
  • Garcia-Viguera, C. 1992. Composition of propolis from two different Spanish region. Z. Naturforsch., 47c: 634-637.
  • Ghısalbertı, E., 1979. Propolis: A Rewiev. Bee World 60:59- 84.
  • Hayashi, K., Komura, S., Isajı, N., Ohıshı, N., Yagı, K., 1999. İsolation of antioksidative compounds from Brazilian propolis: 3, 4-dihydroxy-5-prenylcinnamic acid, a novel potent antioxidant. Chem. Pharm. Bull., 47 (11): 1521-1524.
  • Ikeno, K., Ikeno, T., Miyazawa, C. 1991. Effects of propolis on dental caries in rats. Carries Res., 25: 347-351.
  • Jorgensen, J. H., Ferraro, M. J., Craig, W. A., Doern, G., Finegold, S. M., FungTom, C., J., Hansen, S. L., Hindler, J., Reller, L. B., Swenson, J. M. ; Tenover, F. C., Testa, R. T., Wikler, M. A., 1997. Guidance on Review Criteria for Assesment of antimicrobial susceptibility devices. National Commitee for Clinical Laboratory Standards, Mrch 8, 2000. (www. nccls. org), 26p.
  • Kujumgiev, A., Bankova, V., Ignatova, A., Popov, S. 1999. Antibacterial activity of propolis, some of its components and their analogs. Pharmazie 48: 785-786.
  • Marcucci, M. C., Ferreres, F., Garcia-Viguera C., Bankova V. S., De Castro, S. L., Dantas, A. P., Valente, P. H. M., Paulino, N. 2001. Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of ethnopharmacology 74:105-112.
  • Metzner, J., Bekemeıer, H., Paintz, M., Schneidewind, E. 1979. Zur antimikrobiellen Wirksamkeit von propolis und propolisinhaltsstoffen. Pharmazie, 34: 97-102.
  • Mıtamura, T., Matsuno, T., Sakamoto, S., Maemura, M., Kudo, H., Suzuki, S., Kuwa, K., Yoshımura, S., Sassa, S., Nakayama, T., Nagasawa, H. 1996. Effects of a new clerodane diterpenoid isolated from propolis on chemically induced skin tumors in mice. Anticancer Research, 16: 2699-2672.
  • Ota, C. Unterkircher, C., Fantinato, V., Shimuzu, M. T. 2001. Antifungal activity of propolis on different species of Candida. Mycoses 44: 375-378.
  • Öztürk, F., Kurt, E., İnan, U. U., Emiroğlu, L., İlker, S. S. 1999. The effects of acetylcholine and propolis extract on corneal epithelial wound healing in rats. Cornea, 18(4): 466-471.
  • Sforcin, J. M., Fernandes Jr, A., Lopes, C. A. M., Bankova, V., Funari, S. R. C., 2000. Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology, 73:243-249.
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Details

Primary Language Turkish
Subjects Structural Biology
Journal Section Research Articles
Authors

Dr. Sibel Silici This is me

Prof. Dr. Osman Kaftanoğlu This is me

Publication Date August 26, 2003
Published in Issue Year 2003 Volume: 03 Issue: 3

Cite

Vancouver Silici DS, Kaftanoğlu PDO. Türkiye’nin Farklı Coğrafik Bölgelerinden Toplanan Propolis Örneklerinin Antimikrobiyel Analizi. U. Arı. D.-U. Bee J. 2003;03(3):16-8.

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