Antifungal Activity of Propolis Extract Against Certain Phytopathogenic Fungi

Year 2003, Volume: 03 Issue: 3, 35 - 37, 26.08.2003

Yrd. Doç. Dr. Şener Kurt Yrd. Doç. Dr. Nuray Şahinler

Abstract

The antifungal effects of 7 concentrations (0.0, 0.03, 0.06, 0.125, 0.25, 0.5, and 1 ppm) of propolis ethanol extract (PEE) against Verticillium dahliae Kleb., Fulvia fulva (Cooke) Cif., and Penicillium digitatum Sacc. was investigated in vitro. Propolis was mixed alone or in combination with potato dextrose agar (PDA) medium at various concentrations. The results indicated that the mycelial growth of the tested fungi decreased with each increase in PEE concentrations. The effectiveness of the propolis on V. dahliae was 84.8 % and 83.3 % at 1.0 ppm and 0.5 ppm concentrations, while it was 52.1 % and 33.9 % at 0.06 ppm and 0.03 ppm, respectively. Propolis extract had an effect 80.1 % at the highest concentration against Fulvia fulva. The lowest concentration of propolis (0.03 ppm) was ineffective with 19.3 %. The effect of ethanol extract of propolis on Penicillium digitatum was 86.2 % and 79.3 % at 1.0 ppm and 0.5 ppm concentrations, respectively. The lowest concentration of propolis (0.03 ppm) had an effect 29.4 %.

Keywords

Propolis, Verticillium dahliae, Fulvia fulva, Penicillium digitatum

Propolis Ekstraktının Bitki Patojeni Funguslara Karşı Antifungal Aktivitesi

Abstract

Propolis etanol ekstraktının (PEE) 7 farklı konsantrasyonunun (0.0, 0.03, 0.06, 0.25, 0.5, ve 1 ppm) Verticillium dahliae Kleb., Fulvia fulva (Cooke) Cif., ve Penicillium digitatum Sacc. ’a karşı antifungal etkileri in vitro koşullarda araştırılmıştır. Propolis, farklı konsantrasyonlarda PDA besi ortamına karıştırılmış veya tekli olarak kullanılmıştır. Deneme sonucunda, PEE’nin artan konsantrasyonlarında, denemeye alınan fungusların miselyal gelişiminde azalma ortaya çıktığı belirlenmiştir. Propolisin V. dahliae üzerine etkinliği 1.0 ve 0.5 ppm konsantrasyonlarında sırasıyla % 84.8 ve % 83.3 olurken 0.06 ve 0.03 ppm’de % 2.1 ve % 33.9 olarak saptanmıştır. Propolis ekstraktı, en yüksek konsantrasyonda Fulvia fulva’ya karşı % 80.1 oranında etkili bulunmuştur. Propolisin en düşük konsantrasyonu (0.03 ppm), % 19.3 oranında etkisiz olmuştur. Propolisin etanol ekstraktının P. digitatum üzerine etkisi, 1. 0 ppm ve 0. 5 ppm konsantrasyonlarında sırasıyla % 86.2 ve % 79.3 olarak saptanmıştır. Anahtar Kelimeler: Propolis, Verticillium dahliae, Fulvia fulva, Penicillium digitatum,

Keywords

Propolis, Verticillium dahliae, Fulvia fulva, Penicillium digitatum

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