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Antifungal Activity of Propolis Extract Against Certain Phytopathogenic Fungi

Year 2003, Volume: 03 Issue: 3, 35 - 37, 26.08.2003

Abstract

The antifungal effects of 7 concentrations (0.0, 0.03, 0.06, 0.125, 0.25, 0.5, and 1 ppm) of propolis ethanol extract (PEE) against Verticillium dahliae Kleb., Fulvia fulva (Cooke) Cif., and Penicillium digitatum Sacc. was investigated in vitro. Propolis was mixed alone or in combination with potato dextrose agar (PDA) medium at various concentrations. The results indicated that the mycelial growth of the tested fungi decreased with each increase in PEE concentrations. The effectiveness of the propolis on V. dahliae was 84.8 % and 83.3 % at 1.0 ppm and 0.5 ppm concentrations, while it was 52.1 % and 33.9 % at 0.06 ppm and 0.03 ppm, respectively. Propolis extract had an effect 80.1 % at the highest concentration against Fulvia fulva. The lowest concentration of propolis (0.03 ppm) was ineffective with 19.3 %. The effect of ethanol extract of propolis on Penicillium digitatum was 86.2 % and 79.3 % at 1.0 ppm and 0.5 ppm concentrations, respectively. The lowest concentration of propolis (0.03 ppm) had an effect 29.4 %.

References

  • Abdulsalam, K. S., 1995. Bioactivity of propolis extract against certain soilborne fungi. Alexandria Journal of Agricultural Research, 40: 3, 305-313.
  • Bankova, V. S., S. L de Castro, M. C. Marcucci, 2000. Propolis; recent advantages in chemistry and plant origin. Apidologie 31, 3-15.
  • Bell, A. A., 1992. Verticillium wilt. In: Compendium of Cotton Diseases. G. M. Watkins, ed., APS, St. Paul, MN, Pp. 41-44.
  • Bicici, M., Kurt, S., 1998. Etiology, incidence and prevalence of cotton wilt disease and strains of the wilt pathogen in Cukurova. Proceedings of World Cotton Research Conference-2, ”New Frontiers in Cotton Research”, (Volume II), Athens, Greece, pp. 914-918.
  • Canıhos, Y., Kurt, S., Bicici, M., Erkılıc, A., 1997. Induction of phytoalexing gossypol production of cotton plants treated with some herbicides and its antifungal activity. 4 th. Balkan Conference on Operational Research, Volume of Abstracts, 20- 23 October, Thessaloniki, Greece.
  • Esentepe, M., Karcılıoglu, A., Sezgin, E., Onan, E., 1986. Investigations on relation between the severity of cotton wilt disease (Verticillium dahliae Kleb. ) and yield loss in Aegean region. J. Turkish Phytopathology 15 (1): 27-36.
  • Fahmy, F. G., and Omar, M. O. M., 1989. Potential use of propolis to control white rot disease of onion. Assiut Journal of Agricultural Sciences, 20 (1): 265-275.
  • Hassanein, F. M., 1997. Evaluation of antimicrobial activity of some bee products against certain phytopathogenic bacteria and fungi. Alexandria Journal of Agricultural Research, 42; 3, 239-250.
  • Jones, J. B., and Jones, J. P., 1993. Leaf mold. In: Compendium of Tomato Diseases, eds., J. B. Jones, J. P. Jones, R. A. Stall, T. A. Zitter. APS Press, pp. 18.
  • Krell, R., 1996. Value-added products from beekeeping. FAO Agricultural Services Bulletin, No. 124. Rome, Italy.
  • Kurt, S., Bicici, M., 1998. Development of Verticillium dahliae in cotton plants grown in Cukurova and reaction of the some cultivars to the wilt. World Cotton Research Conference-2 (New Frontiers in Cotton Research), Vol II, Athens, Greece, p. 919-922 .
  • Lindenfelser, L. A., 1967. Antimicrobial activity of propolis. Amer. Bee J., 107, 90-92, 130-131.
  • Özcan, M. 1999. Antifungal properties of propolis. Grasas y Aceites, 50, 395-398.
  • Toker, S., Kurt, S., Canihos, Y., Erkılıc, A., Bicici, M. 1995. Limonlarda hasat sonrası mavi ve yeşil küf çürüklüklerine karşı Imazalil ile daldırma uygulamalarının etkinliği (Effectiveness of Imazalil-dipping treatment against postharvest blue and green mould rots of lemon). 2nd National Horticultural Congress of Turkey (Volume I), p. 576-580.

Propolis Ekstraktının Bitki Patojeni Funguslara Karşı Antifungal Aktivitesi

Year 2003, Volume: 03 Issue: 3, 35 - 37, 26.08.2003

Abstract

Propolis etanol ekstraktının (PEE) 7 farklı konsantrasyonunun (0.0, 0.03, 0.06, 0.25, 0.5, ve 1 ppm) Verticillium dahliae Kleb., Fulvia fulva (Cooke) Cif., ve Penicillium digitatum Sacc. ’a karşı antifungal etkileri in vitro koşullarda araştırılmıştır. Propolis, farklı konsantrasyonlarda PDA besi ortamına karıştırılmış veya tekli olarak kullanılmıştır. Deneme sonucunda, PEE’nin artan konsantrasyonlarında, denemeye alınan fungusların miselyal gelişiminde azalma ortaya çıktığı belirlenmiştir. Propolisin V. dahliae üzerine etkinliği 1.0 ve 0.5 ppm konsantrasyonlarında sırasıyla % 84.8 ve % 83.3 olurken 0.06 ve 0.03 ppm’de % 2.1 ve % 33.9 olarak saptanmıştır. Propolis ekstraktı, en yüksek konsantrasyonda Fulvia fulva’ya karşı % 80.1 oranında etkili bulunmuştur. Propolisin en düşük konsantrasyonu (0.03 ppm), % 19.3 oranında etkisiz olmuştur. Propolisin etanol ekstraktının P. digitatum üzerine etkisi, 1. 0 ppm ve 0. 5 ppm konsantrasyonlarında sırasıyla % 86.2 ve % 79.3 olarak saptanmıştır. Anahtar Kelimeler: Propolis, Verticillium dahliae, Fulvia fulva, Penicillium digitatum,

References

  • Abdulsalam, K. S., 1995. Bioactivity of propolis extract against certain soilborne fungi. Alexandria Journal of Agricultural Research, 40: 3, 305-313.
  • Bankova, V. S., S. L de Castro, M. C. Marcucci, 2000. Propolis; recent advantages in chemistry and plant origin. Apidologie 31, 3-15.
  • Bell, A. A., 1992. Verticillium wilt. In: Compendium of Cotton Diseases. G. M. Watkins, ed., APS, St. Paul, MN, Pp. 41-44.
  • Bicici, M., Kurt, S., 1998. Etiology, incidence and prevalence of cotton wilt disease and strains of the wilt pathogen in Cukurova. Proceedings of World Cotton Research Conference-2, ”New Frontiers in Cotton Research”, (Volume II), Athens, Greece, pp. 914-918.
  • Canıhos, Y., Kurt, S., Bicici, M., Erkılıc, A., 1997. Induction of phytoalexing gossypol production of cotton plants treated with some herbicides and its antifungal activity. 4 th. Balkan Conference on Operational Research, Volume of Abstracts, 20- 23 October, Thessaloniki, Greece.
  • Esentepe, M., Karcılıoglu, A., Sezgin, E., Onan, E., 1986. Investigations on relation between the severity of cotton wilt disease (Verticillium dahliae Kleb. ) and yield loss in Aegean region. J. Turkish Phytopathology 15 (1): 27-36.
  • Fahmy, F. G., and Omar, M. O. M., 1989. Potential use of propolis to control white rot disease of onion. Assiut Journal of Agricultural Sciences, 20 (1): 265-275.
  • Hassanein, F. M., 1997. Evaluation of antimicrobial activity of some bee products against certain phytopathogenic bacteria and fungi. Alexandria Journal of Agricultural Research, 42; 3, 239-250.
  • Jones, J. B., and Jones, J. P., 1993. Leaf mold. In: Compendium of Tomato Diseases, eds., J. B. Jones, J. P. Jones, R. A. Stall, T. A. Zitter. APS Press, pp. 18.
  • Krell, R., 1996. Value-added products from beekeeping. FAO Agricultural Services Bulletin, No. 124. Rome, Italy.
  • Kurt, S., Bicici, M., 1998. Development of Verticillium dahliae in cotton plants grown in Cukurova and reaction of the some cultivars to the wilt. World Cotton Research Conference-2 (New Frontiers in Cotton Research), Vol II, Athens, Greece, p. 919-922 .
  • Lindenfelser, L. A., 1967. Antimicrobial activity of propolis. Amer. Bee J., 107, 90-92, 130-131.
  • Özcan, M. 1999. Antifungal properties of propolis. Grasas y Aceites, 50, 395-398.
  • Toker, S., Kurt, S., Canihos, Y., Erkılıc, A., Bicici, M. 1995. Limonlarda hasat sonrası mavi ve yeşil küf çürüklüklerine karşı Imazalil ile daldırma uygulamalarının etkinliği (Effectiveness of Imazalil-dipping treatment against postharvest blue and green mould rots of lemon). 2nd National Horticultural Congress of Turkey (Volume I), p. 576-580.
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Details

Primary Language Turkish
Subjects Structural Biology
Journal Section Research Articles
Authors

Yrd. Doç. Dr. Şener Kurt This is me

Yrd. Doç. Dr. Nuray Şahinler This is me

Publication Date August 26, 2003
Published in Issue Year 2003 Volume: 03 Issue: 3

Cite

Vancouver Kurt YDDŞ, Şahinler YDDN. Propolis Ekstraktının Bitki Patojeni Funguslara Karşı Antifungal Aktivitesi. U. Arı. D.-U. Bee J. 2003;03(3):35-7.

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