The Effects of Propolis on Chalkbrood (Ascospheraapis) Disease in Honey Bee Colonies

Year 2003, Volume: 03 Issue: 4, 37 - 39, 25.11.2003

Nuray Şahinler Şener Kurt Osman Kaftanoğlu

Abstract

The effectiveness of Turkish propolis on the pathogen of chalkbrood disease (Ascosphera apis) was studied. The extract was prepared by mixing 1900 ml 70% ethanol and 100 g propolis. Isolated A.apis pathogen were cultured in PDA (Potato Dextrose Agar). Five mm in diameter A.apis culture discs were placed in the petri dishes containing PDA and 50 ppm, 25ppm, 12.5 ppm, 6.25 ppm, 3.125 ppm and 1.56 ppm of 5% propolis extract and incubated at 31 ± 1 °C. The growth of the pathogen was evaluated after the 1 month of incubation period. Propolis extracts was found to be highly effective against to A.apis pathogen in vitro conditions. Keywords: Honeybee, propolis, Ascosphera apis,

Keywords

Honeybee, propolis, Ascosphera apis

Propolisin Kireç Hastalığı Üzerine Etkileri

Abstract

Bu çalışma popolisin Kireç hastalığı etmeni (Ascosphera apis) üzerine etkilerini belirlemek üzere yapılmıştır. Propolis ekstratı 1900 ml % 70 lik etil alkol ve 100 g propolis ile hazırlanmıştır. Đsole edilmiş olana A.apis patojeni PDA (patates Dekstroa Agar)da kültüre alınmıştır. 5 mm2 çapındaki A.apis kültür diskleri içinde PDA ve 50,25, 12.5, 6.25, 3.125 ppm % 5lik proplis ekstratı bulunan petri kutuları içinde 31 ± 1 °C inkübasyona bırakılmıştır. Bir aylık inkübasyon peryodundan sonra patojenin gelişimi değerlendirilmiştir. Propolis ekstratının in vitro koşullarda A.apis patojenine karşı yüksek bir etkiye sahip olduğu belirlenmiştir.

Keywords

Bal arısı, propolis, Ascosphera apis

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