BEE POLLEN: ITS ANTIOXIDANT ACTIVITY

Year 2021, Volume: 21 Issue: 1, 119 - 131, 12.05.2021

Hidayet Tutun , Muhammet Mükerrem Kaya , Melike Sultan Usluer , Hatice Ahu Kahraman

https://doi.org/10.31467/uluaricilik.896045

Cited By: 11

Abstract

Bee pollen is a honey bee product containing over 250 biologically active substances such as phenolic bases, amino acids, carbohydrates, lipids, enzymes and coenzymes, vitamins and bio-elements. The composition of bee pollen may vary due to plant sources and its botanical and geographical origin. Bee pollen has been used since ancient times in traditional medicine for its therapeutic effects such as wound healing and hepatoprotective. Bee pollen has been reported to possess antioxidant and radical scavenging activities usually attributed to the presence of phenolic acids and flavonoids which are plant-derived polyphenolic substances. The antioxidant capacity of bee pollen depends on the content of total polyphenolic substances. This review presents an overview of chemical composition and antioxidant activity of bee pollen.

Keywords

Antioxidant activity, Bee pollen, Oxidative stress

Arı Poleni: Antioksidan Etkisi

Abstract

Arı poleni fenolik bazlar, amino asitler, karbonhidratlar, lipitler, enzimler ve koenzimler, vitaminler ve biyo-elementler gibi 250'den fazla biyolojik olarak aktif madde içeren bir arı ürünüdür. Polen bileşimi polenin bitkisel kaynağına, botanik ve coğrafi kökenine göre değişebilir. Arı poleni, yara iyileştirici ve karaciğer koruyucu gibi etkileri nedeniyle geleneksel tıpta eski çağlardan beri kullanılmaktadır. Arı poleninin, genellikle bitkilerden elde edilen polifenolik maddeler olan fenolik asitlerin ve flavonoidlerin varlığına atfedilen antioksidan ve radikal temizleme aktivitelerine sahip olduğu bildirilmiştir. Antioksidan kapasitesi, toplam polifenolik maddelerin içeriğine bağlıdır. Bu derleme arı poleninin kimyasal bileşimi ve antioksidan aktivitesine genel bir bakış sunmaktadır.
Anahtar Kelimeler: Antioksidan aktivite, Arı poleni, Oksidatif stres,

Keywords

Antioksidan aktivite, Arı poleni, Oksidatif stres

References

• Adaškevičiūtė, V., Kaškonienė, V., Kaškonas, P., Barčauskaitė, K., Maruška. A. 2019. Comparison of physicochemical properties of bee pollen with other bee products. Biomolecules, 9(12): 819., doi.org/10.3390/biom9120819
• Adaškevičiūtė, V., Kaškonienė, V., Kaškonas, P., Barčauskaitė, K., Maruška. A. 2019. Comparison of physicochemical properties of bee pollen with other bee products. Biomolecules, 9(12): 819., doi.org/10.3390/biom9120819
• Aguilar, TAF., Navarro, BCH., Perez, JAM. 2016. Endogenous antioxidants: a review of their role in oxidative stress. In: Morales-Gonzalez JA, Morales-González A, Madrigal-Santillan EO (editors). A master regulator of oxidative stress-the transcription factor nrf2. London, IntechOpen. pp. 4-19. ISBN: 978-953-51-2838-0 doi.org/10.5772/65715
• Aguilar, TAF., Navarro, BCH., Perez, JAM. 2016. Endogenous antioxidants: a review of their role in oxidative stress. In: Morales-Gonzalez JA, Morales-González A, Madrigal-Santillan EO (editors). A master regulator of oxidative stress-the transcription factor nrf2. London, IntechOpen. pp. 4-19. ISBN: 978-953-51-2838-0 doi.org/10.5772/65715
• Aličić, D., Šubarić, D., Jašić, M., Pašalić, H., Ačkar Đ. 2014. Antioxidant properties of pollen HRČAK, 3(1): 6-12.
• Aličić, D., Šubarić, D., Jašić, M., Pašalić, H., Ačkar Đ. 2014. Antioxidant properties of pollen HRČAK, 3(1): 6-12.
• Atsalakis, E., Chinou, I., Makropoulou, M., Karabournioti, S., Graikou, K. 2017. Evaluation of phenolic compounds in Cistus creticus bee pollen from Greece. Antioxidant and antimicrobial properties Nat. Prod. Commun, 12(11):1813-1816., doi.org/10.1177/1934578X1701201141
• Atsalakis, E., Chinou, I., Makropoulou, M., Karabournioti, S., Graikou, K. 2017. Evaluation of phenolic compounds in Cistus creticus bee pollen from Greece. Antioxidant and antimicrobial properties Nat. Prod. Commun, 12(11):1813-1816., doi.org/10.1177/1934578X1701201141
• Birben, E., Sahiner, UM., Sackesen, C., Erzurum, S., Kalayci, O. 2012. Oxidative stress and antioxidant defense World Allergy Organ J, 5(1): 9-19., doi.org/10.1097/WOX.0b013e3182439613
• Birben, E., Sahiner, UM., Sackesen, C., Erzurum, S., Kalayci, O. 2012. Oxidative stress and antioxidant defense World Allergy Organ J, 5(1): 9-19., doi.org/10.1097/WOX.0b013e3182439613
• Campos, MG., Bogdanov, S., de Almeida-Muradian, LB., Szczesna, T., Mancebo, Y., Frigerio, C., Ferreira, F. 2008. Pollen composition and standardisation of analytical methods J. Apic. Res., 47(2): 154-161., doi.org/10.1080/00218839.2008.11101443
• Campos, MG., Bogdanov, S., de Almeida-Muradian, LB., Szczesna, T., Mancebo, Y., Frigerio, C., Ferreira, F. 2008. Pollen composition and standardisation of analytical methods J. Apic. Res., 47(2): 154-161., doi.org/10.1080/00218839.2008.11101443
• Campos, MG., Webby, RF., Markham, KR., Mitchell, KA., Da Cunha, AP. 2003. Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids J. Agric. Food Chem, 51(3): 742-745., doi.org/10.1021/jf0206466.
• Campos, MG., Webby, RF., Markham, KR., Mitchell, KA., Da Cunha, AP. 2003. Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids J. Agric. Food Chem, 51(3): 742-745., doi.org/10.1021/jf0206466.
• Carpes, ST., Mourão, GB., De Alencar, SM., Masson, ML. 2009. Chemical composition and free radical scavenging activity of Apis mellifera bee pollen from Southern Brazil Brazilian J. Food Technol, 12(03) 220-229., doi.org/10.4260/BJFT2009800900016
• Carpes, ST., Mourão, GB., De Alencar, SM., Masson, ML. 2009. Chemical composition and free radical scavenging activity of Apis mellifera bee pollen from Southern Brazil Brazilian J. Food Technol, 12(03) 220-229., doi.org/10.4260/BJFT2009800900016
• Cho, KJ., Seo, JM., Kim, JH. 2011. Bioactive lipoxygenase metabolites stimulation of NADPH oxidases and reactive oxygen species Mol Cells. 32(1): 1-5., doi.org/10.1007/s10059-011-1021-7
• Cho, KJ., Seo, JM., Kim, JH. 2011. Bioactive lipoxygenase metabolites stimulation of NADPH oxidases and reactive oxygen species Mol Cells. 32(1): 1-5., doi.org/10.1007/s10059-011-1021-7
• Cornara, L., Biagi, M., Xiao, J., Burlando, B. 2017. Therapeutic properties of bioactive compounds from different honeybee products Front Pharmacol. 8: 412., doi.org/10.3389/fphar.2017.00412
• Cornara, L., Biagi, M., Xiao, J., Burlando, B. 2017. Therapeutic properties of bioactive compounds from different honeybee products Front Pharmacol. 8: 412., doi.org/10.3389/fphar.2017.00412
• de Florio Almeida, J., dos Reis, AS., Heldt, LFS., Pereira, D., Bianchin, M., de Moura, C., Plata-Oviedo, MV., Haminiuk, CWI., Riberio, IS., Pinto da Luz, CF., Carpes, ST. 2017. Lyophilized bee pollen extract: A natural antioxidant source to prevent lipid oxidation in refrigerated sausages LBWTAP. 76(Part B): 299-305., doi.org/10.1016/j.lwt.2016.06.017
• de Florio Almeida, J., dos Reis, AS., Heldt, LFS., Pereira, D., Bianchin, M., de Moura, C., Plata-Oviedo, MV., Haminiuk, CWI., Riberio, IS., Pinto da Luz, CF., Carpes, ST. 2017. Lyophilized bee pollen extract: A natural antioxidant source to prevent lipid oxidation in refrigerated sausages LBWTAP. 76(Part B): 299-305., doi.org/10.1016/j.lwt.2016.06.017
• De‐Melo, AAM., Estevinho, LM., Moreira, MM., Delerue‐Matos, C., Freitas, ADSD., Barth, OM., Almeida‐Muradian, LBD. 2018. Phenolic profile by HPLC‐MS, biological potential, and nutritional value of a promising food: Monofloral bee pollen J. Food Biochem 42(5), e12536., https://doi.org/10.1111/jfbc.12536
• De‐Melo, AAM., Estevinho, LM., Moreira, MM., Delerue‐Matos, C., Freitas, ADSD., Barth, OM., Almeida‐Muradian, LBD. 2018. Phenolic profile by HPLC‐MS, biological potential, and nutritional value of a promising food: Monofloral bee pollen J. Food Biochem 42(5), e12536., https://doi.org/10.1111/jfbc.12536
• Denisow, B., Denisow‐Pietrzyk, M. 2016. Biological and therapeutic properties of bee pollen: a review J Sci Food Agric. 96 (13):4303-4309., doi.org/10.1002/jsfa.7729
• Denisow, B., Denisow‐Pietrzyk, M. 2016. Biological and therapeutic properties of bee pollen: a review J Sci Food Agric. 96 (13):4303-4309., doi.org/10.1002/jsfa.7729
• Dulger Altiner, D., Sandikci Altunatmaz, S., Sabuncu, M., Aksu, F., Sahan, Y. 2020. In-vitro bioaccessibility of antioxidant properties of bee pollen in Turkey Food Sci (AHEAD). doi.org/10.1590/fst.10220
• Dulger Altiner, D., Sandikci Altunatmaz, S., Sabuncu, M., Aksu, F., Sahan, Y. 2020. In-vitro bioaccessibility of antioxidant properties of bee pollen in Turkey Food Sci (AHEAD). doi.org/10.1590/fst.10220
• Fatiha, M., Abdelkader, T. 2019. Study of antioxidant activity of pyrimidinium betaines by DPPH radical scavenging method J Anal Pharm Res. 8: 33-36., doi.org/10.15406/japlr.2019.08.00308
• Fatiha, M., Abdelkader, T. 2019. Study of antioxidant activity of pyrimidinium betaines by DPPH radical scavenging method J Anal Pharm Res. 8: 33-36., doi.org/10.15406/japlr.2019.08.00308
• Fatrcová-Šramková, K., Nôžková, J., Máriássyová, M., Kačániová, M. 2016. Biologically active antimicrobial and antioxidant substances in the Helianthus annuus L. bee pollen J Environ Sci Health B. Part B, 51(3):176-181., doi.org/10.1080/03601234.2015.1108811
• Fatrcová-Šramková, K., Nôžková, J., Máriássyová, M., Kačániová, M. 2016. Biologically active antimicrobial and antioxidant substances in the Helianthus annuus L. bee pollen J Environ Sci Health B. Part B, 51(3):176-181., doi.org/10.1080/03601234.2015.1108811
• Feás, X., Vázquez-Tato, MP., Estevinho, L., Seijas, JA., Iglesias, A. 2012. Organic bee pollen: botanical origin, nutritional value, bioactive compounds, antioxidant activity and microbiological quality Molecules. 17(7): 8359-8377., doi.org/10.3390/molecules17078359
• Feás, X., Vázquez-Tato, MP., Estevinho, L., Seijas, JA., Iglesias, A. 2012. Organic bee pollen: botanical origin, nutritional value, bioactive compounds, antioxidant activity and microbiological quality Molecules. 17(7): 8359-8377., doi.org/10.3390/molecules17078359
• Gabriele, M., Parri, E., Felicioli, A., Sagon, S., Pozzo, L., Biondi, C., Domenici, V., Pucci, L. 2015. Phytochemical composition and antioxidant activity of Tuscan bee pollen of different botanic origins Ital. J. Food Saf. 27(2):248-259., doi.org/10.14674/1120-1770/ijfs.v191
• Gabriele, M., Parri, E., Felicioli, A., Sagon, S., Pozzo, L., Biondi, C., Domenici, V., Pucci, L. 2015. Phytochemical composition and antioxidant activity of Tuscan bee pollen of different botanic origins Ital. J. Food Saf. 27(2):248-259., doi.org/10.14674/1120-1770/ijfs.v191
• Guiné, RPF. 2015. Bee Pollen: Chemical Composition and Potential Beneficial Effects on Health Curr Nutr Food Sci, 11(4):301-308., doi.org/10.2174/1573401311666150630181615
• Guiné, RPF. 2015. Bee Pollen: Chemical Composition and Potential Beneficial Effects on Health Curr Nutr Food Sci, 11(4):301-308., doi.org/10.2174/1573401311666150630181615
• Guo, C., Sun, L., Chen, X., Zhang, D. 2013. Oxidative stress, mitochondrial damage and neurodegenerative diseases Neural Regen Res. 8(21):2003–2014., doi.org/10.3969/j.issn.1673-5374.2013.21.009
• Guo, C., Sun, L., Chen, X., Zhang, D. 2013. Oxidative stress, mitochondrial damage and neurodegenerative diseases Neural Regen Res. 8(21):2003–2014., doi.org/10.3969/j.issn.1673-5374.2013.21.009
• Harif Fadzilah, N., Jaapar, MF., Jajuli, R., Wan Omar, WA. 2017. Total phenolic content, total flavonoid and antioxidant activity of ethanolic bee pollen extracts from three species of Malaysian stingless bee J. Apic. Res. 56(2): 130-135., doi.org/10.1080/00218839.2017.1287996
• Harif Fadzilah, N., Jaapar, MF., Jajuli, R., Wan Omar, WA. 2017. Total phenolic content, total flavonoid and antioxidant activity of ethanolic bee pollen extracts from three species of Malaysian stingless bee J. Apic. Res. 56(2): 130-135., doi.org/10.1080/00218839.2017.1287996
• He, L., He, T., Farrar, S., Ji, L., Liu, T., Ma, X. 2017. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species Cell Physiol Biochem, 44(2):532-553., doi.org/10.1159/000485089
• He, L., He, T., Farrar, S., Ji, L., Liu, T., Ma, X. 2017. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species Cell Physiol Biochem, 44(2):532-553., doi.org/10.1159/000485089
• Kanar, Y., Mazı, BG. 2019. Effect of different drying methods on antioxidant characteristics of bee-pollen Meas. 13:3376-3386., doi.org/10.1007/s11694-019-00283-5
• Kanar, Y., Mazı, BG. 2019. Effect of different drying methods on antioxidant characteristics of bee-pollen Meas. 13:3376-3386., doi.org/10.1007/s11694-019-00283-5
• Karkar, B., Şahin, S., Güneş, ME. 2020. Evaluation of antioxidant properties and determination of phenolic and carotenoid profiles of chestnut bee pollen collected from Turkey J. Apic. Res. 1-10., doi.org/10.1080/00218839.2020.1844462
• Karkar, B., Şahin, S., Güneş, ME. 2020. Evaluation of antioxidant properties and determination of phenolic and carotenoid profiles of chestnut bee pollen collected from Turkey J. Apic. Res. 1-10., doi.org/10.1080/00218839.2020.1844462
• Kaškonienė, V., Ruočkuvienė, G., Kaškonas, P., Akuneca, I., Maruška, A. 2015. Chemometric analysis of bee pollen based on volatile and phenolic compound compositions and antioxidant properties Food Anal. Methods. 8(5):1150-1163., doi.org/10.1007/s12161-014-9996-2
• Kaškonienė, V., Ruočkuvienė, G., Kaškonas, P., Akuneca, I., Maruška, A. 2015. Chemometric analysis of bee pollen based on volatile and phenolic compound compositions and antioxidant properties Food Anal. Methods. 8(5):1150-1163., doi.org/10.1007/s12161-014-9996-2
• Kim, SB., Jo, YH., Liu, Q., Ahn, JH., Hong, IP., Han, SM., Hwang, BY., Lee, MK. 2015. Optimization of extraction condition of bee pollen using response surface methodology: correlation between anti-melanogenesis, antioxidant activity, and phenolic content Molecules. 20(11):19764-19774., doi.org/10.3390/molecules201119656
• Kim, SB., Jo, YH., Liu, Q., Ahn, JH., Hong, IP., Han, SM., Hwang, BY., Lee, MK. 2015. Optimization of extraction condition of bee pollen using response surface methodology: correlation between anti-melanogenesis, antioxidant activity, and phenolic content Molecules. 20(11):19764-19774., doi.org/10.3390/molecules201119656
• Kocot, J., Kiełczykowska, M., Luchowska-Kocot, D., Kurzepa, J., Musik, I. 2018. Antioxidant potential of propolis, bee pollen, and royal jelly: Possible medical application Oxid. 7074209., doi.org/10.1155/2018/7074209
• Kocot, J., Kiełczykowska, M., Luchowska-Kocot, D., Kurzepa, J., Musik, I. 2018. Antioxidant potential of propolis, bee pollen, and royal jelly: Possible medical application Oxid. 7074209., doi.org/10.1155/2018/7074209
• Komosinska-Vassev, K., Olczyk, P., Kaźmierczak, J., Mencner, L., Olczyk, K. 2015. Bee pollen: chemical composition and therapeutic application J Evid Based Complementary Altern Med. 2015., doi.org/10.1155/2015/297425
• Komosinska-Vassev, K., Olczyk, P., Kaźmierczak, J., Mencner, L., Olczyk, K. 2015. Bee pollen: chemical composition and therapeutic application J Evid Based Complementary Altern Med. 2015., doi.org/10.1155/2015/297425
• Kuciel-Lewandowska, J., Kasperczak, M., Bogut, B., Heider, R., Laber, WT., Laber, W., Paprocka-Borowicz, M. 2020. The Impact of Health Resort Treatment on the Nonenzymatic Endogenous Antioxidant System Oxid. 2020(147):1-9., doi.org/10.1155/2020/8423105
• Kuciel-Lewandowska, J., Kasperczak, M., Bogut, B., Heider, R., Laber, WT., Laber, W., Paprocka-Borowicz, M. 2020. The Impact of Health Resort Treatment on the Nonenzymatic Endogenous Antioxidant System Oxid. 2020(147):1-9., doi.org/10.1155/2020/8423105
• LeBlanc, BW., Davis, OK., Boue, S., DeLucca, A., Deeby, T. 2009. Antioxidant activity of Sonoran Desert bee pollen Food Chem. 115(4):1299-1305., doi.org/10.1016/j.foodchem.2009.01.055
• LeBlanc, BW., Davis, OK., Boue, S., DeLucca, A., Deeby, T. 2009. Antioxidant activity of Sonoran Desert bee pollen Food Chem. 115(4):1299-1305., doi.org/10.1016/j.foodchem.2009.01.055
• Leja, M., Mareczek, A., Wyżgolik, G., Klepacz-Baniak, J., Czekońska, K. 2007. Antioxidative properties of bee pollen in selected plant species Food Chem. 100(1):237-240., doi.org/10.1016/j.foodchem.2005.09.047
• Leja, M., Mareczek, A., Wyżgolik, G., Klepacz-Baniak, J., Czekońska, K. 2007. Antioxidative properties of bee pollen in selected plant species Food Chem. 100(1):237-240., doi.org/10.1016/j.foodchem.2005.09.047
• Lobo, V., Patil, A., Phatak, A., Chandra, N. 2010. Free radicals, antioxidants and functional foods: Impact on human health Pharmacogn Rev. 4(8): 118-126., doi.org/10.4103/0973-7847.70902
• Lobo, V., Patil, A., Phatak, A., Chandra, N. 2010. Free radicals, antioxidants and functional foods: Impact on human health Pharmacogn Rev. 4(8): 118-126., doi.org/10.4103/0973-7847.70902
• Masella, R., Di Benedetto, R., Varì, R., Filesi, C., Giovannini, C. 2005. Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes J Nutr Biochem. 16(10): 577-586., doi.org/10.1016/j.jnutbio.2005.05.013
• Masella, R., Di Benedetto, R., Varì, R., Filesi, C., Giovannini, C. 2005. Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione-related enzymes J Nutr Biochem. 16(10): 577-586., doi.org/10.1016/j.jnutbio.2005.05.013
• Mărghitaschedilla, LA., Stanciu, OG., Dezmirean, DS., Bobiscedilla, O., Popescu, O., Bogdanov, S., Campos, MG. 2009. In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chem. 115(3):878-883., doi.org/10.1016/j.foodchem.2009.01.014
• Mărghitaschedilla, LA., Stanciu, OG., Dezmirean, DS., Bobiscedilla, O., Popescu, O., Bogdanov, S., Campos, MG. 2009. In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chem. 115(3):878-883., doi.org/10.1016/j.foodchem.2009.01.014
• Mayda, N., Özkök, A., Bayram, NE., Gerçek, YC., Sorkun, K. 2020. Bee bread and bee pollen of different plant sources: determination of phenolic content, antioxidant activity, fatty acid and element profiles Meas. 14(4): 795–1809., doi.org/10.1007/s11694-020-00427-y.
• Mayda, N., Özkök, A., Bayram, NE., Gerçek, YC., Sorkun, K. 2020. Bee bread and bee pollen of different plant sources: determination of phenolic content, antioxidant activity, fatty acid and element profiles Meas. 14(4): 795–1809., doi.org/10.1007/s11694-020-00427-y.
• Mohdaly, AA., Mahmoud, AA., Roby, MH., Smetanska, I., Ramadan, MF. 2015. Phenolic extract from propolis and bee pollen: composition, antioxidant and antibacterial activities J. Food Biochem. 39(4): 538-547., doi.org/10.1111/jfbc.12160.
• Mohdaly, AA., Mahmoud, AA., Roby, MH., Smetanska, I., Ramadan, MF. 2015. Phenolic extract from propolis and bee pollen: composition, antioxidant and antibacterial activities J. Food Biochem. 39(4): 538-547., doi.org/10.1111/jfbc.12160.
• Moniruzzaman, M., Khalil, MI., Sulaiman, SA., Gan, SH. 2011. Advances in the analytical methods for determining the antioxidant properties of honey: a review Afr J Tradit Complement Altern Med. 9(1): 36–42., doi.org/10.4314/ajtcam.v9i1.5.
• Moniruzzaman, M., Khalil, MI., Sulaiman, SA., Gan, SH. 2011. Advances in the analytical methods for determining the antioxidant properties of honey: a review Afr J Tradit Complement Altern Med. 9(1): 36–42., doi.org/10.4314/ajtcam.v9i1.5.
• Okan, OT., Varlibaş, H., Öz, M., Deniz, İ. 2013. Antioksidan analiz yöntemleri ve doğu karadeniz bölgesinde antioksidan kaynağı olarak kullanılabilecek odun dışı bazı bitkisel ürünler Kastamonu Üniv. Orman Fak. Derg. 13(1): 48-59.
• Okan, OT., Varlibaş, H., Öz, M., Deniz, İ. 2013. Antioksidan analiz yöntemleri ve doğu karadeniz bölgesinde antioksidan kaynağı olarak kullanılabilecek odun dışı bazı bitkisel ürünler Kastamonu Üniv. Orman Fak. Derg. 13(1): 48-59.
• Olczyk, P., Koprowski, R., Kaźmierczak, J., Mencner, L., Wojtyczka, R., Stojko, J, Olczyk, K., Komosinska-Vassev, K. 2016. Bee pollen as a promising agent in the burn wounds treatment Evid Based Complement Alternat Med. 2016: 8473937., doi.org/10.1155/2016/8473937
• Olczyk, P., Koprowski, R., Kaźmierczak, J., Mencner, L., Wojtyczka, R., Stojko, J, Olczyk, K., Komosinska-Vassev, K. 2016. Bee pollen as a promising agent in the burn wounds treatment Evid Based Complement Alternat Med. 2016: 8473937., doi.org/10.1155/2016/8473937
• Oliveira, KC., Moriya, M., Azedo, RA., Almeida-Muradian, LBD., Teixeira, EW., Alves, ML., Moreti, AC. 2009. Relationship between botanical origin and antioxidants vitamins of bee-collected pollen Química Nova, 32(5):1099-1102., doi.org/10.1590/S0100-40422009000500003
• Oliveira, KC., Moriya, M., Azedo, RA., Almeida-Muradian, LBD., Teixeira, EW., Alves, ML., Moreti, AC. 2009. Relationship between botanical origin and antioxidants vitamins of bee-collected pollen Química Nova, 32(5):1099-1102., doi.org/10.1590/S0100-40422009000500003
• Oliveira, RGD., Jain, S., Freitas, LDS., Araújo, EDD. 2019. Phenolic compound, nutritional and antioxidant profile of pollen collected by the genus melipona in North Eastern Brazil Brazilian J. Food Technol. 22(4)., doi.org/10.1590/1981-6723.07918
• Oliveira, RGD., Jain, S., Freitas, LDS., Araújo, EDD. 2019. Phenolic compound, nutritional and antioxidant profile of pollen collected by the genus melipona in North Eastern Brazil Brazilian J. Food Technol. 22(4)., doi.org/10.1590/1981-6723.07918
• Özcan, MM., Aljuhaimi, F., Babiker, EE., Uslu, N., Ceylan, DA., Ghafoor, K., Özcan, MM., Dursun, N., Ahmed, IM., Fadimu, GJ., Alsawmahi, ON. 2019. Determination of antioxidant activity, phenolic compound, mineral contents and fatty acid compositions of bee pollen grains collected from different locations J. Apic. Res. 63(1): 69-79., doi.org/10.2478/jas-2019-0004
• Özcan, MM., Aljuhaimi, F., Babiker, EE., Uslu, N., Ceylan, DA., Ghafoor, K., Özcan, MM., Dursun, N., Ahmed, IM., Fadimu, GJ., Alsawmahi, ON. 2019. Determination of antioxidant activity, phenolic compound, mineral contents and fatty acid compositions of bee pollen grains collected from different locations J. Apic. Res. 63(1): 69-79., doi.org/10.2478/jas-2019-0004
• Özkök, D., Silici, S. 2017. Antioxidant activities of honeybee products and their mixtures. Food Sci Biotechnol. 26(1): 201-206., doi.org/10.1007/s10068-017-0027-0
• Özkök, D., Silici, S. 2017. Antioxidant activities of honeybee products and their mixtures. Food Sci Biotechnol. 26(1): 201-206., doi.org/10.1007/s10068-017-0027-0
• Panche, AN., Diwan, AD., Chandra, SR. 2016. Flavonoids: an overview J Nutr Sci. 5: 1-5., doi.org/10.1017/jns.2016.41.
• Panche, AN., Diwan, AD., Chandra, SR. 2016. Flavonoids: an overview J Nutr Sci. 5: 1-5., doi.org/10.1017/jns.2016.41.
• Pascoal, A., Rodrigues, S., Teixeira, A., Feás, Estevinh, LM. 2014. Biological activities of commercial bee pollens: Antimicrobial, antimutagenic, antioxidant and anti-inflammatory Food Chem Toxicol. 63: 233-239., doi.org/10.1016/j.fct.2013.11.010.
• Pascoal, A., Rodrigues, S., Teixeira, A., Feás, Estevinh, LM. 2014. Biological activities of commercial bee pollens: Antimicrobial, antimutagenic, antioxidant and anti-inflammatory Food Chem Toxicol. 63: 233-239., doi.org/10.1016/j.fct.2013.11.010.
• Pérez-Pérez, EM., Vit, P., Rivas, E., Sciortino, R., Sosa, A., Tejada, D., Rodríguez-Malaver, AJ. 2012. Antioxidant activity of four color fractions of bee pollen from Mérida, Venezuela Arch Latinoam Nutr. 62: 375-80., PMID: 24020258.
• Pérez-Pérez, EM., Vit, P., Rivas, E., Sciortino, R., Sosa, A., Tejada, D., Rodríguez-Malaver, AJ. 2012. Antioxidant activity of four color fractions of bee pollen from Mérida, Venezuela Arch Latinoam Nutr. 62: 375-80., PMID: 24020258.
• Pham-Huy, LA., He, H., Pham-Huy, C. 2008. Free radicals, antioxidants in disease and health Int J Biomed Sci. 4: 89–96., PMID: 23675073
• Pham-Huy, LA., He, H., Pham-Huy, C. 2008. Free radicals, antioxidants in disease and health Int J Biomed Sci. 4: 89–96., PMID: 23675073
• Phaniendra, A., Jestadi, DB., Periyasamy L. 2015. Free radicals: properties, sources, targets, and their implication in various diseases Indian J Clin Biochem. 30(1):11-26., doi.org/10.1007/s12291-014-0446-0
• Phaniendra, A., Jestadi, DB., Periyasamy L. 2015. Free radicals: properties, sources, targets, and their implication in various diseases Indian J Clin Biochem. 30(1):11-26., doi.org/10.1007/s12291-014-0446-0
• Pisoschi, AM., Negulescu, GP. 2011. Methods for total antioxidant activity determination: a review Biochem. Analytical Biochemistry. 1(1):106., doi.org/10.4172/2161-1009.1000106
• Pisoschi, AM., Negulescu, GP. 2011. Methods for total antioxidant activity determination: a review Biochem. Analytical Biochemistry. 1(1):106., doi.org/10.4172/2161-1009.1000106
• Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., Bitto, A. 2017. Oxidative Stress: Harms and Benefits for Human Health Oxid Med Cell Longev. 2017, 8416763., doi.org/10.1155/2017/8416763
• Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., Bitto, A. 2017. Oxidative Stress: Harms and Benefits for Human Health Oxid Med Cell Longev. 2017, 8416763., doi.org/10.1155/2017/8416763
• Rao, PS., Kalva, S., Yerramilli, A., Mamid, S. 2011. Free radicals and tissue damage: Role of antioxidants Free Radic Biol Med. 1(4): 2-7., doi.org/10.4103/0973-7847.70902
• Rao, PS., Kalva, S., Yerramilli, A., Mamid, S. 2011. Free radicals and tissue damage: Role of antioxidants Free Radic Biol Med. 1(4): 2-7., doi.org/10.4103/0973-7847.70902
• Rocchetti, G., Castiglioni, S., Maldarizzi, G., Carloni, P., Lucini, L. 2019. UHPLC‐ESI‐QTOF‐MS phenolic profiling and antioxidant capacity of bee pollen from different botanical origin J. Food Sci. Technol. 54: 335-346., doi.org/10.1111/ijfs.13941
• Rocchetti, G., Castiglioni, S., Maldarizzi, G., Carloni, P., Lucini, L. 2019. UHPLC‐ESI‐QTOF‐MS phenolic profiling and antioxidant capacity of bee pollen from different botanical origin J. Food Sci. Technol. 54: 335-346., doi.org/10.1111/ijfs.13941
• Romero, AC., Hernández, EGO., Cerón, TF., Chávez, AÁ. 2013. The exogenous antioxidants. Ed.: Jose Antonio Morales-Gonzalez. Oxidative Stress and Chronic Degenerative Diseases-A Role for Antioxidants, IntechOpen, London, UK. 33. doi.org/10.5772/52490
• Romero, AC., Hernández, EGO., Cerón, TF., Chávez, AÁ. 2013. The exogenous antioxidants. Ed.: Jose Antonio Morales-Gonzalez. Oxidative Stress and Chronic Degenerative Diseases-A Role for Antioxidants, IntechOpen, London, UK. 33. doi.org/10.5772/52490
• Saral, Ö., Kilicarslan, M., Şahin, H., Yildiz, O., Dincer, B. 2019. Evaluation of antioxidant activity of bee products of different bee races in Turkey. Turk J Vet Anim Sci. 43(4): 441-447., doi.org/10.3906/vet-1901-3
• Saral, Ö., Kilicarslan, M., Şahin, H., Yildiz, O., Dincer, B. 2019. Evaluation of antioxidant activity of bee products of different bee races in Turkey. Turk J Vet Anim Sci. 43(4): 441-447., doi.org/10.3906/vet-1901-3
• Šarić, A., Balog, T., Sobočanec, S., Kušić, B., Šverko, V., Rusak, G., Likić, S., Bubalo, D., Pinto, B., Reali, D., Marotti, T. 2009. Antioxidant effects of flavonoid from Croatian Cystus incanus L. rich bee pollen. Food Chem Toxicol. 47(3): 547-554., doi.org/10.1016/j.fct.2008.12.007
• Šarić, A., Balog, T., Sobočanec, S., Kušić, B., Šverko, V., Rusak, G., Likić, S., Bubalo, D., Pinto, B., Reali, D., Marotti, T. 2009. Antioxidant effects of flavonoid from Croatian Cystus incanus L. rich bee pollen. Food Chem Toxicol. 47(3): 547-554., doi.org/10.1016/j.fct.2008.12.007
• Sharma, P., Jha, AB., Dubey, RS., Pessarakli, M. 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions Turk J Bot. 2012., doi.org/10.1155/2012/217037
• Sharma, P., Jha, AB., Dubey, RS., Pessarakli, M. 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions Turk J Bot. 2012., doi.org/10.1155/2012/217037
• Soares de Arruda, VA., Vieria dos Santos, A., Figueiredo Sampaio, D., da Silva Araújo, E., de Castro Peixoto, AL., Estevinho, LM., de Almeida-Muradian, LB. 2020. Brazilian bee pollen: phenolic content, antioxidant properties and antimicrobial activity J. Apic. Res. 1-9., doi.org//10.1080/00218839.2020.1840854
• Soares de Arruda, VA., Vieria dos Santos, A., Figueiredo Sampaio, D., da Silva Araújo, E., de Castro Peixoto, AL., Estevinho, LM., de Almeida-Muradian, LB. 2020. Brazilian bee pollen: phenolic content, antioxidant properties and antimicrobial activity J. Apic. Res. 1-9., doi.org//10.1080/00218839.2020.1840854
• Sun, L., Guo, Y., Zhang, Y., Zhuang, Y. 2017. Antioxidant and anti-tyrosinase activities of phenolic extracts from rape bee pollen and inhibitory melanogenesis by cAMP/MITF/TYR pathway in B16 mouse melanoma cells Front Pharmacol. 8:104., doi.org/10.3389/fphar.2017.00104
• Sun, L., Guo, Y., Zhang, Y., Zhuang, Y. 2017. Antioxidant and anti-tyrosinase activities of phenolic extracts from rape bee pollen and inhibitory melanogenesis by cAMP/MITF/TYR pathway in B16 mouse melanoma cells Front Pharmacol. 8:104., doi.org/10.3389/fphar.2017.00104
• Thakur, M., Nanda, V. 2020. Composition and functionality of bee pollen: A review Trends Food Sci Technol. 98: 82-106., doi.org/10.1016/j.tifs.2020.02.001
• Thakur, M., Nanda, V. 2020. Composition and functionality of bee pollen: A review Trends Food Sci Technol. 98: 82-106., doi.org/10.1016/j.tifs.2020.02.001
• Ulusoy, E., Kolayli, S. 2014. Phenolic composition and antioxidant properties of Anzer bee pollen J. Food Biochem. 38(1): 73-82., doi.org/10.1111/jfbc.12027
• Ulusoy, E., Kolayli, S. 2014. Phenolic composition and antioxidant properties of Anzer bee pollen J. Food Biochem. 38(1): 73-82., doi.org/10.1111/jfbc.12027
• Velasquez, P., Rodriguez, K., Retamal, M., Giordano, A., Valenzuela, LM., Montenegro, G. 2017. Relation between composition, antioxidant and antibacterial activities and botanical origin of multi-floral bee pollen J. Appl. Bot. Food Qual. 90(1): 306-314., doi.org/10.5073/JABFQ.2017.090.038
• Velasquez, P., Rodriguez, K., Retamal, M., Giordano, A., Valenzuela, LM., Montenegro, G. 2017. Relation between composition, antioxidant and antibacterial activities and botanical origin of multi-floral bee pollen J. Appl. Bot. Food Qual. 90(1): 306-314., doi.org/10.5073/JABFQ.2017.090.038
• Villanueva, MO., Marquina, AD., Serrano, RB., Abellán, GB. 2002. The importance of bee-collected pollen in the diet: a study of its composition Int J Food Sci Nutr. 53(3): 217-224., doi.org/10.1080/09637480220132832.
• Villanueva, MO., Marquina, AD., Serrano, RB., Abellán, GB. 2002. The importance of bee-collected pollen in the diet: a study of its composition Int J Food Sci Nutr. 53(3): 217-224., doi.org/10.1080/09637480220132832.
• Warraich, YA., Hussain, F., Kayani, HUR. 2020. Aging-Oxidative stress, antioxidants and computational modeling Heliyon. 6(5): E04107., doi.org/10.1016/j.heliyon.2020.e04107
• Warraich, YA., Hussain, F., Kayani, HUR. 2020. Aging-Oxidative stress, antioxidants and computational modeling Heliyon. 6(5): E04107., doi.org/10.1016/j.heliyon.2020.e04107
• Yang, Y., Zhang, JL., Zhou, Q., Wang, L., Huang, W., Wang, RD. 2019. Effect of ultrasonic and ball‐milling treatment on cell wall, nutrients, and antioxidant capacity of rose (Rosa rugosa) bee pollen, and identification of bioactive components J Sci Food Agric. 99(12): 5350-5357., doi.org/10.1002/jsfa.9774.
• Yang, Y., Zhang, JL., Zhou, Q., Wang, L., Huang, W., Wang, RD. 2019. Effect of ultrasonic and ball‐milling treatment on cell wall, nutrients, and antioxidant capacity of rose (Rosa rugosa) bee pollen, and identification of bioactive components J Sci Food Agric. 99(12): 5350-5357., doi.org/10.1002/jsfa.9774.