Anadolu Propolisinin Paenibacillus larvae Üzerine Antibakteriyel Etkisi

Year 2021, Volume: 21 Issue: 2, 177 - 186, 11.11.2021

Elif Sevim , Arif Bozdeveci , Müberra Pınarbaş Çetin , Meral Kekeçoğlu , Rahşan Akpınar , Merve Keskin , Sevgi Kolaylı , Şengül Alpay Karaoğlu

https://doi.org/10.31467/uluaricilik.976536

Cited By: 4

Abstract

Paenibacillus larvae (P. larvae), bal arısı larvalarında Amerikan Yavru Çürüklüğü hastalığına (AYÇ) neden olan patojenik bir bakteridir. P. Larvae tedavisinde kullanılan antibiyotikler bal arısı kolonilerinin ciddi kaybına neden olmakta ve bu nedenle antibiyotik kullanımına bağlı olarak bu hastalığın kontrolü için alternatif stratejiler geliştirmek gerekmektedir. Son çalışmalar, P. larvae’ya karşı doğal mücadele ajanlarını araştırmaya yöneliktir. Çalışmamızda Anadolu propolisi etanolik ekstresinin (EAP) Türkiye'de izole edilen P. larvae suşları PB35 ve SV35'e karşı potansiyel antimikrobiyal etkinliğinin ortaya konması amaçlanmıştır. EAP, toplam fenolik madde (TPC), flavonoid madde miktarları (TFC) ve bazı fenolik bileşikler açısından karakterize edilmiştir. Analizlere göre, EAP'nin total fenolik madde miktarı ve flavonoid madde miktarları sırasıyla 181,73±5,20 mg Gallik Asit Eşdeğeri (GAE)/g TFC olarak 42,33±1,40 mg Kuersetin Eşdeğeri (QE)/g ve farklı miktarlarda ferulik, kafeik, kumarik asitler, rutin ve kafeik asit fenetil ester (CAPE) içerdiği bulundu. EAP'nin antimikrobiyal aktivitesi, agar kuyu difüzyon, mikrodilüsyon ve Bioscreen C teknikleri kullanılarak belirlendi. EAP'nin Minimal İnhibisyon Konsantrasyon (MIC) değerleri, hem mikrodilüsyon hem de Bioscreen C tekniği kullanılarak PB35 ve SV35 suşuna karşı 74,87 μg/ml olarak belirlendi. Her iki teknikte de PB35 ve SV35 suşlarına karşı EAP'nin minimal bakterisidal konsantrasyon (MBC) değerleri sırasıyla 149 ve 598,4 μg/ml olarak belirlendi.

Keywords

Amerikan yavru çürüğü, Anadolu propolisi, MBC, MIC, Paenibacillus larvae

Supporting Institution

Recep Tayyip Erdoğan University

Project Number

RTEU-BAP ID 317 and Project number 2015.53001.102.03.04

ANTIBACTERIAL EFFECTS OF ANATOLIAN PROPOLIS ON PAENIBACILLUS LARVAE

Abstract

Paenibacillus larvae (P. larvae) is a pathogenic bacterium causing American Foulbrood Disease (AFB) in honeybee larvae. It is necessary to develop alternative strategies for the control of this disease due to the serious honeybee colonies loses and the use of antibiotics. Recent studies are aimed at the investigating natural fighting agents against P. larvae. In our study, it was aimed to demonstrate potential antibacterial efficacy of ethanol extract of Anatolian Propolis (EAP) against P. larvae strains PB35 and SV35 which were isolated in Turkey. The total phenolic content (TPC) and flavonoid content (TFC) of EEAP were determined as 181.73±5.20 mg Gallic Acid Equivalents (GAE)/g, and 42.33±1.40 mg Quercetin Equivalents (QE)/g, respectively. It was found that EAP contains different amounts of ferulic, caffeic, coumaric acids, rutin, and caffeic acid phenethyl ester (CAPE). The antibacterial activity of the EAP was determined by using agar-well diffusion, microdilution, and Bioscreen C techniques. The Minimal Inhibition Concentration (MIC) values of the EAP were determined as 74.87 μg/ml against strain PB35 and SV35 using both microdilution and Bioscreen C teqnique. In both techniques, Minimal Bactericidal Concentration (MBC) values of the EAP were evaluated as 149 and 598.4 μg/ml against strain PB35 and SV35, respectively. The fact that EAP shows low concentrations of bacteriostatic (MIC) and bactericide (MBC) activity values against P. larvae strains, spore-forming bacilli, which are agents of AFB disease, suggests that it may be a potential source in AFB disease control.

Keywords

American Foulbrood, Anatolian propolis, MBC, MIC, Paenibacillus larvae

Project Number

RTEU-BAP ID 317 and Project number 2015.53001.102.03.04

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