IMMUNOMODULATORY EFFECT OF INDONESIAN PROPOLIS IN PREGNANT MICE: A PRELIMINARY RESULT

Year 2022, Volume: 22 Issue: 1, 68 - 75, 19.05.2022

Al Mukhlas Fikri Ahmad Sulaeman Sri Anna Marliyati Mokhamad Fahrudin Ekowati Handharyani

https://doi.org/10.31467/uluaricilik.1098059

Abstract

Propolis has been widely accepted to exhibit immunomodulatory activity. However, the activity during pregnancy has not been investigated yet. This study was a preliminary study that aimed to analyze the effect of several types of Indonesian propolis on the histological changes of maternal mice spleen. A total of 25 pregnant mice were divided into 5 groups, control (1% Tween 80) group, low (380 mg/kg) and high dose (1400 mg/kg) ethanol extract of South Sulawesi propolis groups, and low (380 mg/kg) and high dose (1400 mg/kg) water extract of Banten propolis groups. Propolis samples were administered daily during pregnancy. At day 18 of gestation, the mice were sacrificed to obtain spleen which was used for histological evaluation using hematoxylin and eosin staining. The number and diameter of white pulp were observed under the 10x magnifying of microscope. The results showed that all propolis extracts at low dose significantly increased the number of white pulp (p < 0.05). However, an increase in the diameter was found not significant in all propolis administered groups. This study suggests that Indonesian propolis may modulate maternal immune system.

Keywords

immunomodulator, histology, pregnancy, propolis

Supporting Institution

The Ministry of Research and Technology and Higher Education of the Republic of Indonesia

Endonezya Propolis'in Hamile Farelerde İmmünomodülatör Etkisi: Bir Ön Sonuç

Abstract

Propolisin immünomodülatör aktiviteye sahip olduğu yaygın olarak kabul edilmiştir. Bununla birlikte, hamilelik sırasındaki aktivite henüz araştırılmamıştır. Bu çalışma, hamilelik sırasında propolis uygulamasının anne fare dalağının histolojik değişiklikleri üzerindeki etkisini analiz etmeyi amaçladı. Toplam 25 hamile fare, Güney Sulawesi propolisinin kontrol (%1 Tween 80), düşük (380 mg/kg) ve yüksek doz (1400 mg/kg) etanol özütü ve düşük (380 mg/kg) ve yüksek dozlu (1400 mg/kg) Banten propolisinin su özütü olmak üzere 5 gruba ayrıldı. Propolis uygulaması gebelik boyunca günlük olarak yapıldı. Gebeliğin 18. gününde fareler, hematoksilen ve eozin boyaması kullanılarak histolojik değerlendirme için kullanılan dalak elde etmek için sakrifiye edildi. Beyaz pulp sayısı ve çapı 10x büyütmeli mikroskop altında gözlendi. Sonuçlar, düşük dozdaki tüm propolis ekstraktlarının beyaz pulp sayısını önemli ölçüde arttırdığını gösterdi (p < 0.05). Bununla birlikte, propolis uygulanan tüm gruplarda çaptaki bir artış anlamlı bulunmadı. Bu çalışma, Endonezya propolisinin annenin bağışıklık sistemini modüle edebileceğini düşündürmektedir.

Keywords

Immunomodulatori, histoloji, hamilelik, propolis

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