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Amasya Bal Arılarında Mikrobiyal Patojenlerin Moleküler Tanımlanması

Year 2023, Volume: 23 Issue: 1, 93 - 104, 19.05.2023
https://doi.org/10.31467/uluaricilik.1254857

Abstract

Bal arıları, Apis mellifera, hem üreticilerin geçimini hem de tüketicilerin besin kaynağını oluşturan oldukça faydalı böceklerdir. Ancak arıların verimini etkileyen ve neredeyse tüm koloninin çökmesine neden olan bazı hastalıklar vardır. Bu hastalıkların çoğuna virüsler, bakteriler ve mantarlardan kaynaklanan mikrobiyal patojenler neden olur. Arıcılık gerek Amasya merkezde gerekse hemen hemen tüm ilçelerinde önemli bir geçim kaynağıdır. Bu çalışmada Amasya ilinde toplu arı ölümlerine ve salgın hastalıklara neden olan mikrobiyal patojenler moleküler yöntemler kullanılarak belirlenmiştir. Sonuçlar, Amasya'da en yaygın bal arısı patojenlerinin Deforme kanat virüsü, Kronik arı felci virüsü ve Aspergillus flavus mantarı olduğunu göstermiştir. Böylece Amasya ilindeki arı hastalıklarının profili ilk kez bu çalışma ile belirlenmiştir. Ayrıca bu çalışma, arı hastalıklarının önlenmesi ve sağlıklı arıcılık için planlanan diğer çalışmalara yol göstermektedir.

References

  • Antunez K, D’Alessandro, B, Corbella E, Ramallo G., Zunino P. Honey bee viruses in Uruguay. Journal of Invertebrate Pathology, 2006;93:67–70.
  • Bailey L, Ball BV, Woods RD. An iridovirus from bees. Journal of General Virolology, 1976;31:459-461.
  • Baker AC, Schroeder DC. The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations. Virology Journal, 2008;5-10.
  • Berenyi, O, Bakonyi T, Derakhshifar I, oglberger H, and Nowotny N. Occurrence of six honeybee viruses in diseased Austrian apiaries. Appl. Environ. Microbiol. 2006;72:2414–2420.
  • Bog EŞ, Ertürk Ö, Yaman M. Pathogenicity of aerobic bacteria isolated from honey bees (Apis mellifera) in Ordu Province. Turkish Journal of Veterinary & Animal Sciences, 2020.;44(3):714-719.
  • Borum AE, Ülgen M. Güney Marmara Bölgesindeki Bal Arılarının Chalkbrood (Ascosphaera apis) İnfeksiyonunda Predispozisyon Faktörleri. Uludağ Arıcılık Dergisi, 2010;10(2):56-69.
  • Chen YP, Pettis JS, Feldlaufer MF. Detection of multiple viruses in queens of the honey bee Apis mellifera L. Journal of Invertebrate Pathology, 2005;90:118-121.
  • Dias CA, Taís Ferreira J, Weinstein Teixeira E, Pedro Lourenco A. Honey bee viruses in solitary bees in South America: simultaneous detection and prevalence. Journal of Apicultural Research, 2023;1-6.
  • Dittes J, Schafer MO, Aupperle-Lellbach H, Mulling CK, Emmerich IU. Overt infection with Chronic Bee Paralysis Virus (CBPV) in two honey bee colonies. Veterinary sciences, 2020;7(3):142.
  • Doğaroğlu M. Modern arıcılık. Anadolu Matbaa ve Ambalaj San. Tic. Ltd. Şti. İstanbul, 1999. Dolezal AG, Toth AL. Feedbacks between nutrition and disease in honey bee health. Current opinion in insect science, 2018;26:114-119.
  • Eroglu GB. Phylogeographic Relationship of Honey Bee Dicistroviruses. Bee World, 2022a; 99(3):99-102.
  • Eroglu GB. RNA Viruses in Honey Bees. In: Distinguished Research from Different Disciplines. Gelenbevi Scientific Research Journal: Announcements. 2022b.;1:133-147.
  • Eroglu GB. Detection of honey bee viruses in Vespula germanica: Black queen cell virus and Kashmir bee virus. Biologia, 2023. doi:10.1007/s11756-023-01416-4.
  • Foley K, Fazio G, Jensen AB, Hughes WO. The distribution of Aspergillus spp. opportunistic parasites in hives and their pathogenicity to honey bees. Veterinary Microbiology, 2014;169:203-210.
  • Galbraith DA, Zachary LF, Allyson MR, Axel B, Maryann F, Mary W.G, J. Francisco IM. Investigating the Viral Ecology of Global Bee Communities with High-Throughput Metagenomics. Scientific Reports, 2018;8:1-14.
  • Ghorani M, Madadgar O, Langeroudi AG, Rezapanah, M, Nabian S, Akbarein H, et al. The irst comprehensive molecular detection of six honey bee viruses in Iran in 2015-2016. Archives of virology, 2017;162: 2287-2291.
  • Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied Environmental Microbiology, 1995;61:1323-30.
  • Gülmez Y, Bursalı A, Tekin S. First molecular detection and characterization of deformed wing virus (DWV) in honey bees (Apis mellifera L.) and mite (Varroa destructor) in Turkey. African Journal of Biotechnology, 2009;8(16).
  • Ilyasov RA, Lee Ml, Takahashi JI, Kwon HW, Nikolenko, A.G. A revision of subspecies structure of western honey bee Apis mellifera. Saudi Journal of Biological Sciences, 2020;27(12):3615-3621.
  • James RR, Skinner JS. PCR diagnostic methods for Ascosphaera infections in bees. Journal of Invertebrate Pathology, 2005;90(2):98-103.
  • Kadirhan C, Kırpık MA, Öziç C, Gülen M. Kars ve Ardahan Yöresi Bal Arısında (Apis mellifera caucasica L.) Bakteriyel Hastalıkların Tespit Edilmesi. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2019;12(1):28-34.
  • Kalaycı G, Çağırgan AA, Pekmez K, Özkan B, Kaplan M. Molecular detection and phylogenetic analysis of the honey bee (Apis mellifera) sacbrood virus in Turkey. Turkish Journal of Veterinary & Animal Sciences, 2019;43(4):551-554.
  • Kalayci G, Cagirgan AA, Kaplan M, Pekmez K, Beyazit A, Ozkan B & Arslan F. The role of viral and parasitic pathogens affected by colony losses in Turkish apiaries. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2020;26(5).
  • Koziy RV, Wood SC, Kozii I., van Rensburg CJ, Moshynskyy I, Dvylyuk I, Simko E. Deformed wing virus infection in honey bees (Apis mellifera L.). Veterinary Pathology, 2019;56(4): 636-641.
  • Le Conte Y, Navajas M. Climate change: impact on honey bee populations and diseases. Revue Scientifique et Technique-Office International des Epizooties, 2008;27:499-510.
  • McMenamin AJ, Flenniken ML. Recently identified bee viruses and their impact on three bee pollinators. Current Opinion Insect Science, 2018; 26:120–129.
  • Muz D, Muz MN. Survey of the occurrence of Deformed Wing Virus and multiple parasites of queens (Apis mellifera L.) in apiaries with collapsed colonies in Hatay, Turkey. Journal of apicultural research, 2009;48(3): 204-208.
  • Muz D, Muz MN. A molecular epidemiological study of black queen cell virus in honey bees (Apis mellifera) of Turkey: the first genetic characterization and phylogenetic analysis of field viruses. Apidologie, 2018;49(1):89-100.
  • Ribiere M, Olivier V, Blanchard P. Chronic bee paralysis: A disease and a virus like no other? Journal of Invertebrate Pathology, 2010;103:120–131.
  • Rüstemoğlu M, Sipahioğlu, HM. Occurrence and molecular characterization of acute bee paralysis virus (ABPV) in honey bee (Apis mellifera) colonies in Hakkari province. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2016.
  • Rüstemoğlu M, Sipahioğlu HM. Occurrence and prevalence of six honey bee viruses in Hakkari (Turkey) and their genomic divergence. Munis Entomology & Zoology, 2019;14(2):574-583.
  • Schoonvaere K, De Smet L, Smagghe G, Vierstraete A., Braeckman BP, de Graaf DC. Study of the Metatranscriptome of Eight Social and Solitary Wild Bee Species Reveals Novel Viruses and Bee Parasites. Published in Front. Microbiol. Biology, Medicine Frontiers in Microbiology, 2018;9:1-12.
  • Şimşek H. Elazığ yöresi bal arılarında bazı parazit ve mantar hastalıklarının araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 2005;52(2):123-126.
  • Tentcheva D, Gauthier L, Zappulla N, Dainat B, Cousserans F. Colin ME, et al. Prevalence and seasonal variations of six bee viruses in Apis mellifera L. And Varroa destructor mite populations in France. Applied Environmental Microbiology, 2004;70:7185–7191.
  • Vojvodic S, Jensen AB, James RR, Boomsma JJ, Eilenberg J. Temperature dependent virulence of obligate and facultative fungal pathogens of honeybee brood. Veterinary microbiology, 2011;149:200-205.
  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 1991;173:697–703.
  • Welch A, Drummond, F, Tewari S, Averill A, Burand, JP. Presence and prevalence of viruses in local and migratory honeybees (Apis mellifera) in Massachusetts. Applied Environmental Microbiology, 2009;75:7862–7865.

MOLECULAR IDENTIFICATION OF MICROBIAL PATHOGENS IN HONEY BEES FROM AMASYA

Year 2023, Volume: 23 Issue: 1, 93 - 104, 19.05.2023
https://doi.org/10.31467/uluaricilik.1254857

Abstract

Honey bees, Apis mellifera are highly beneficial insects that constitute both the livelihood of the producers and the food source of the consumers. However, there are some diseases that affect the yield of bees and cause the collapse of almost the entire colony. Most of these diseases are caused by microbial pathogens originating from viruses, bacteria, and fungi. Beekeeping is an important source of livelihood both in the center of Amasya and in almost all its districts. In this study, microbial pathogens that cause mass bee deaths and epidemics in Amasya province were determined using molecular methods. The results showed that the most common honey bee pathogens in Amasya are the Deformed wing virus, Chronic bee paralysis virus, and Aspergillus flavus fungus. Thus, the profile of bee diseases in Amasya province was determined for the first time with this study. In addition, this study guides other studies planned for the prevention of bee diseases and healthy beekeeping.

References

  • Antunez K, D’Alessandro, B, Corbella E, Ramallo G., Zunino P. Honey bee viruses in Uruguay. Journal of Invertebrate Pathology, 2006;93:67–70.
  • Bailey L, Ball BV, Woods RD. An iridovirus from bees. Journal of General Virolology, 1976;31:459-461.
  • Baker AC, Schroeder DC. The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations. Virology Journal, 2008;5-10.
  • Berenyi, O, Bakonyi T, Derakhshifar I, oglberger H, and Nowotny N. Occurrence of six honeybee viruses in diseased Austrian apiaries. Appl. Environ. Microbiol. 2006;72:2414–2420.
  • Bog EŞ, Ertürk Ö, Yaman M. Pathogenicity of aerobic bacteria isolated from honey bees (Apis mellifera) in Ordu Province. Turkish Journal of Veterinary & Animal Sciences, 2020.;44(3):714-719.
  • Borum AE, Ülgen M. Güney Marmara Bölgesindeki Bal Arılarının Chalkbrood (Ascosphaera apis) İnfeksiyonunda Predispozisyon Faktörleri. Uludağ Arıcılık Dergisi, 2010;10(2):56-69.
  • Chen YP, Pettis JS, Feldlaufer MF. Detection of multiple viruses in queens of the honey bee Apis mellifera L. Journal of Invertebrate Pathology, 2005;90:118-121.
  • Dias CA, Taís Ferreira J, Weinstein Teixeira E, Pedro Lourenco A. Honey bee viruses in solitary bees in South America: simultaneous detection and prevalence. Journal of Apicultural Research, 2023;1-6.
  • Dittes J, Schafer MO, Aupperle-Lellbach H, Mulling CK, Emmerich IU. Overt infection with Chronic Bee Paralysis Virus (CBPV) in two honey bee colonies. Veterinary sciences, 2020;7(3):142.
  • Doğaroğlu M. Modern arıcılık. Anadolu Matbaa ve Ambalaj San. Tic. Ltd. Şti. İstanbul, 1999. Dolezal AG, Toth AL. Feedbacks between nutrition and disease in honey bee health. Current opinion in insect science, 2018;26:114-119.
  • Eroglu GB. Phylogeographic Relationship of Honey Bee Dicistroviruses. Bee World, 2022a; 99(3):99-102.
  • Eroglu GB. RNA Viruses in Honey Bees. In: Distinguished Research from Different Disciplines. Gelenbevi Scientific Research Journal: Announcements. 2022b.;1:133-147.
  • Eroglu GB. Detection of honey bee viruses in Vespula germanica: Black queen cell virus and Kashmir bee virus. Biologia, 2023. doi:10.1007/s11756-023-01416-4.
  • Foley K, Fazio G, Jensen AB, Hughes WO. The distribution of Aspergillus spp. opportunistic parasites in hives and their pathogenicity to honey bees. Veterinary Microbiology, 2014;169:203-210.
  • Galbraith DA, Zachary LF, Allyson MR, Axel B, Maryann F, Mary W.G, J. Francisco IM. Investigating the Viral Ecology of Global Bee Communities with High-Throughput Metagenomics. Scientific Reports, 2018;8:1-14.
  • Ghorani M, Madadgar O, Langeroudi AG, Rezapanah, M, Nabian S, Akbarein H, et al. The irst comprehensive molecular detection of six honey bee viruses in Iran in 2015-2016. Archives of virology, 2017;162: 2287-2291.
  • Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied Environmental Microbiology, 1995;61:1323-30.
  • Gülmez Y, Bursalı A, Tekin S. First molecular detection and characterization of deformed wing virus (DWV) in honey bees (Apis mellifera L.) and mite (Varroa destructor) in Turkey. African Journal of Biotechnology, 2009;8(16).
  • Ilyasov RA, Lee Ml, Takahashi JI, Kwon HW, Nikolenko, A.G. A revision of subspecies structure of western honey bee Apis mellifera. Saudi Journal of Biological Sciences, 2020;27(12):3615-3621.
  • James RR, Skinner JS. PCR diagnostic methods for Ascosphaera infections in bees. Journal of Invertebrate Pathology, 2005;90(2):98-103.
  • Kadirhan C, Kırpık MA, Öziç C, Gülen M. Kars ve Ardahan Yöresi Bal Arısında (Apis mellifera caucasica L.) Bakteriyel Hastalıkların Tespit Edilmesi. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 2019;12(1):28-34.
  • Kalaycı G, Çağırgan AA, Pekmez K, Özkan B, Kaplan M. Molecular detection and phylogenetic analysis of the honey bee (Apis mellifera) sacbrood virus in Turkey. Turkish Journal of Veterinary & Animal Sciences, 2019;43(4):551-554.
  • Kalayci G, Cagirgan AA, Kaplan M, Pekmez K, Beyazit A, Ozkan B & Arslan F. The role of viral and parasitic pathogens affected by colony losses in Turkish apiaries. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 2020;26(5).
  • Koziy RV, Wood SC, Kozii I., van Rensburg CJ, Moshynskyy I, Dvylyuk I, Simko E. Deformed wing virus infection in honey bees (Apis mellifera L.). Veterinary Pathology, 2019;56(4): 636-641.
  • Le Conte Y, Navajas M. Climate change: impact on honey bee populations and diseases. Revue Scientifique et Technique-Office International des Epizooties, 2008;27:499-510.
  • McMenamin AJ, Flenniken ML. Recently identified bee viruses and their impact on three bee pollinators. Current Opinion Insect Science, 2018; 26:120–129.
  • Muz D, Muz MN. Survey of the occurrence of Deformed Wing Virus and multiple parasites of queens (Apis mellifera L.) in apiaries with collapsed colonies in Hatay, Turkey. Journal of apicultural research, 2009;48(3): 204-208.
  • Muz D, Muz MN. A molecular epidemiological study of black queen cell virus in honey bees (Apis mellifera) of Turkey: the first genetic characterization and phylogenetic analysis of field viruses. Apidologie, 2018;49(1):89-100.
  • Ribiere M, Olivier V, Blanchard P. Chronic bee paralysis: A disease and a virus like no other? Journal of Invertebrate Pathology, 2010;103:120–131.
  • Rüstemoğlu M, Sipahioğlu, HM. Occurrence and molecular characterization of acute bee paralysis virus (ABPV) in honey bee (Apis mellifera) colonies in Hakkari province. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, 2016.
  • Rüstemoğlu M, Sipahioğlu HM. Occurrence and prevalence of six honey bee viruses in Hakkari (Turkey) and their genomic divergence. Munis Entomology & Zoology, 2019;14(2):574-583.
  • Schoonvaere K, De Smet L, Smagghe G, Vierstraete A., Braeckman BP, de Graaf DC. Study of the Metatranscriptome of Eight Social and Solitary Wild Bee Species Reveals Novel Viruses and Bee Parasites. Published in Front. Microbiol. Biology, Medicine Frontiers in Microbiology, 2018;9:1-12.
  • Şimşek H. Elazığ yöresi bal arılarında bazı parazit ve mantar hastalıklarının araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 2005;52(2):123-126.
  • Tentcheva D, Gauthier L, Zappulla N, Dainat B, Cousserans F. Colin ME, et al. Prevalence and seasonal variations of six bee viruses in Apis mellifera L. And Varroa destructor mite populations in France. Applied Environmental Microbiology, 2004;70:7185–7191.
  • Vojvodic S, Jensen AB, James RR, Boomsma JJ, Eilenberg J. Temperature dependent virulence of obligate and facultative fungal pathogens of honeybee brood. Veterinary microbiology, 2011;149:200-205.
  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology, 1991;173:697–703.
  • Welch A, Drummond, F, Tewari S, Averill A, Burand, JP. Presence and prevalence of viruses in local and migratory honeybees (Apis mellifera) in Massachusetts. Applied Environmental Microbiology, 2009;75:7862–7865.
There are 37 citations in total.

Details

Primary Language English
Subjects Entomology, Agricultural Engineering (Other), Veterinary Surgery
Journal Section Research Articles
Authors

Nese Gul Utkan This is me 0000-0001-6405-3986

Gözde Büşra Eroğlu 0000-0001-8988-1315

Publication Date May 19, 2023
Acceptance Date May 4, 2023
Published in Issue Year 2023 Volume: 23 Issue: 1

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Vancouver Utkan NG, Eroğlu GB. MOLECULAR IDENTIFICATION OF MICROBIAL PATHOGENS IN HONEY BEES FROM AMASYA. U. Arı. D.-U. Bee J. 2023;23(1):93-104.

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