INFLUENCE OF ALUMINUM OXIDE NANOPARTICLES ON BIOLOGICAL FEATURES AND HOST HEMOCYTES OF Galleria mellonella L. (Lepidoptera: Pyralidae) WITH ITS ENDOPARASITOID Pimpla turionellae L. (Hymenoptera: Ichneumonidae)

Year 2024, Volume: 24 Issue: 2, 197 - 208, 18.11.2024

Ezgi Çoğal Zülbiye Demirtürk , Fevzi Uçkan

https://doi.org/10.31467/uluaricilik.1475411

Abstract

Nanoparticles (NPs) are released directly or indirectly into nature with increased production and consumption, and their effects on insects, which occupy a large place in the ecosystem, are of interest. There is also interest in the potentially toxic effects of NPs applied to hive pests on parasitoids, honey bees, and host-parasitoid relationships. The influence of aluminum oxide (Al2O3) NPs on the biological features of the hive pest Galleria mellonella, total counts of hemocyte, and hemocyte types; as well as on the biological features of the endoparasitoid Pimpla turionellae were investigated. The data obtained revealed that Al2O3 NPs caused a decrease in the larval, pupal, and adult development time of G. mellonella. The immature developmental time of P. turionellae was reduced. It was also demonstrated that Al2O3 NPs decreased the total counts of hemocytes in G. mellonella larvae; granulocyte, spherulocyte, oenocytoid, and prohemocyte counts decreased at all NP concentrations, while plasmatocyte counts increased. The data showed that Al2O3 NPs affected the biological properties of the hive pest model organism G. mellonella and indirectly affected its endoparasitoid P. turionellae. In addition, Al2O3 NPs showed a suppressive effect on cellular immune system responses, decreasing hemocyte counts. Our study results suggest that honey bees, honeycomb pests, and parasitoids may be negatively affected by NPs, which have increased in recent years as environmental pollutants, and that NPs may have insecticidal effects.

Keywords

Aluminium oxide nanoparticle, Biological features, Galleria mellonella, Hemocyte, Pimpla turionellae

Supporting Institution

Kocaeli University

Project Number

2020-2296

Thanks

Tuğba Nur ELLİBEŞ GÖKKAYA

Alüminyum Oksit Nanopartiküllerinin Galleria mellonella L. (Lepidoptera: Pyralidae) ile Endoparazitoiti Pimpla turionellae L. (Hymenoptera: Ichneumonidae)’nın Biyolojik Özellikleri ve Konak Hemositleri Üzerine Etkisi

Abstract

Dünya çapında üretim ve tüketimin artmasıyla birlikte nanopartiküller (NP’ler) doğrudan ya da dolaylı olarak doğaya salınmaktadır ve ekosistemde büyük bir yer kaplayan böceklerde etkileri merak uyandırmaktadır. Ayrıca kovan zararlısına uygulanan NP’lerin parazitoitler üzerinde muhtemel toksik etkileri, diğer bir deyişle bal arıları ve konak-parazitoit ilişkileri ilgi çekmektedir. Bu nedenle alüminyum oksit (Al2O3) NP’lerin kovan zararlısı Galleria mellonella’nın biyolojik özellikleri, toplam hemosit sayısı ve hemosit tipleri ile endoparazitoid Pimpla turionellae’nın biyolojik özellikleri üzerindeki etkisi araştırıldı. Elde edilen veriler, Al2O3 NP'lerin G. mellonella’nın larva, pupa ve ergin gelişim sürelerinde azalmaya neden olduğunu ortaya koydu. P. turionellae’nın ise olgunlaşma öncesi gelişim süresi kısaldı. Aynı zamanda Al2O3 NP'lerin G. mellonella larvalarındaki toplam hemosit sayısını azalttığı; granülosit, sferülosit, önositoid ve prohemosit sayılarının tüm NP konsantrasyonlarında azaldığı, plazmatosit sayılarının ise arttığı tespit edildi. Bulgular, Al2O3 NP'lerin kovan zararlısı model organizma G. mellonella’nın biyolojik özelliklerini etkilediğini ve endoparazitoiti P. turionellae’nın dolaylı olarak etkilendiğini gösterdi. Ayrıca Al2O3 NP'lerin hücresel bağışıklık sistemi tepkileri arasında yer alan hemosit sayılarının azalması ile sonuçlanarak baskılayıcı etki gösterdiği görüldü. Çalışma sonuçlarımız bal arılarının, petek zararlılarının ve parazitoitlerinin çevresel kirleticiler olarak son yıllarda artan NP’lerden olumsuz etkileneceği ve NP’lerin insektisidal etki gösterebileceği düşüncesini ortaya koymaktadır.

Keywords

Alüminyum oksit nanopartikülü, Biyolojik özellikler, Galleria mellonella, Hemosit, Pimpla turionellae

Supporting Institution

Kocaeli Üniversitesi

Project Number

2020-2296

Thanks

Tuğba Nur ELLİBEŞ GÖKKAYA

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