ASSESSMENT EFFICIENCY OF CHINESE, AND LOCAL PROPOLIS AGAINST THE GREATER, AND LESSER WAX MOTHS IN HONEYBEE (APIS MELLIFERA, L.) COLONIES

Year 2025, Volume: 25 Issue: 1, 140 - 157, 30.05.2025

Samy Sakr , Mohammed El-basiony Mahmoud Omar Hatem Mahfouz

https://doi.org/10.31467/uluaricilik.1638254

Abstract

This study analyzed the biochemical composition and effectiveness of Chinese and local propolis extracts against greater (Galleria mellonella L.) and lesser (Achroia Grisella, L.) wax moth larvae, while also assessing their impact on adult honeybee workers. Propolis extracts were prepared at 10% concentration using ethanol, acetone, olive oil, water, thyme, and sage at 90%, 50%, and 25% concentrations. Biochemical analysis revealed Chinese propolis contained higher total flavonoids (2.47%), amino acids (13.69%), and lipids (31.36 mg/g) than local propolis (0.95%, 6.21%, and 26.96 mg/g, respectively). Laboratory experiments showed that increasing extract concentration (90%>50%>25%) generally increased mortality in wax moth larvae and adult workers for both propolis types. Chinese propolis extracts were more effective against both wax moth species than local extracts across all concentrations. However, Chinese propolis also caused higher mortality in adult workers, especially at higher concentrations. Ethanolic and olive oil extracts were most effective against wax moth larvae but also negatively affected adult worker survival compared to other extracts.

Keywords

Propolis, Biochemical analysis, Extracts, Wax moths, Alternative control

Bal Arısı (Apis mellifera, L.) Koloni̇leri̇nde Büyük ve Küçük Balmumu Güveleri̇ne Karşi Çin ve Yerel Propoli̇sin Etki̇nli̇ği̇ni̇n Değerlendi̇ri̇lmesi̇

Abstract

Bu çalışmada, Çin ve yerel propolis ekstraktlarının biyokimyasal bileşimi ve büyük (Galleria mellonella L.) ve küçük (Achroia Grisella, L.) balmumu güvesi larvalarına karşı etkinliği analiz edilirken, yetişkin bal arısı işçileri üzerindeki etkileri de değerlendirilmiştir. Propolis ekstraktları %90, %50 ve %25 konsantrasyonlarda etanol, aseton, zeytinyağı, su, kekik ve adaçayı kullanılarak %10 konsantrasyonda hazırlanmıştır. Biyokimyasal analizler Çin propolisinin yerel propolise göre (sırasıyla %0,95, %6,21 ve 26,96 mg/g) daha yüksek toplam flavonoid (%2,47), amino asit (%13,69) ve lipid (31,36 mg/g) içerdiğini ortaya koymuştur. Laboratuvar deneyleri, artan ekstrakt konsantrasyonunun (%90>%50>%25) her iki propolis türü için de balmumu güvesi larvalarında ve yetişkin işçilerde ölüm oranını genel olarak artırdığını göstermiştir. Çin propolisi ekstraktları her iki mum güvesi türüne karşı da tüm konsantrasyonlarda yerel ekstraktlardan daha etkili olmuştur. Bununla birlikte, Çin propolisi özellikle yüksek konsantrasyonlarda yetişkin işçilerde daha yüksek ölüm oranına neden olmuştur. Etanolik ve zeytinyağı özütleri balmumu güvesi larvalarına karşı en etkili özütler olmakla birlikte, diğer özütlere kıyasla yetişkin işçilerin hayatta kalmasını olumsuz yönde etkilemiştir.

Keywords

Propolis, Biyokimyasal analiz, Ekstraktlar, Balmumu güveleri, Alternatif kontrol

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