A comparative study of diagnosis methods for detection of Helicobacter pylori in gastro-duodenal diseases in Human

Year 2015, , 64 - 67, 01.07.2015

Fiaz Ahamed N.r. Chandrappa Emad M. Abdallah

https://doi.org/10.5455/umj.20151009012034

Abstract

Background: Helicopacter pylori play an important role in the pathogenesis of gastroduodenal disease. Appropriate diagnosis of H.pylori infection in the laboratory results in relief of the symptoms and many also be cured without surgical intervention. Method: 76 males and 24 females who underwent diagnostic endoscopy formed the study group. Microscopic examination, culturing, rapid urease test and ELISA test were carried out. Results: Among 100 cases study group suffering from gastroduodenal diseases, H. pylori isolated from 18 cases, identified in 54 grams stained smears, rapid urease test was positive in 62 cases, IgG immunoglobulins against H. pylori was detected in 62 cases. Conclusion: No single test can be considered sensitive or specific to detect or rule out H. pylori infection and it is necessary to use a combination of tests.

Keywords

Keywords: Helicopacter pylori, diagnosis, gram staining, rapid urease test, ELISA.

References

• Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. , 1(8390):1311-1315.
• Steer H.W. Ultrastructures of cell migration through the gastric epithelium and its relationship to bacteria. J Clin Path. , 28: 63-70. Subbukesavaraja V.,.Balan K. Comparative study of invasive methods for diagnosis of Helicobacter pylori in humans. International journal of current microbiology and applied sciences. 2013, 2(7): 63-68.
• International Agency for Research on Cancer: Shistosomes, Liver flukes and Helicobacter pylori.IARC Monographs on the evaluation of carcinogenic risk to humans. 1994. Vol.6 Iyon IARC.
• Goodwin C.S., Armstrong J.A., Chivers T. et al. Transfer of Campylobacter pylori and Campylobacter mustelase to Helicobacter gen nov and H. pylori comb, nov and Helicobaacter mustelase comb. Nov, respectively. Int. J. Syst. Bacteriol. 1989, 39: 397-405.
• Hazell S.L., Barody T.J. Gal A., et al. C. pylori detection of urease as a marker of bacterial colonization and gastritis. Am. J. Gastroenterol, 1987. 82: 292-296.
• Goodwin C.S., Armstrong J.A. Microbiological aspects of H. pylori. Eur. J. Clin. Microbiol. Infect. Dis., 1990, 9: 1-13.
• Skirrow M.B. Isolation of C. pylori antibiotic supplementation. British Medical Journal. 1990, 2-9.
• Axon A.T.R. The transmission of Helicobacteri pylori, which theory fits the facts?.Eur. J. Gastroenterol. Hepatol. 1996. 8: 1-2.
• Goodman K.J., Correu P. The transmission of Helicobacter pylori. A critical review of the evidence. Int. J. Epidemiol. 1995, : 875-887.
• Suvak B., Dulger A.C., Suvak O., Aytemiz E., Kemik O. The prevalence of helicobacter pylori among dyspeptic patients in an earthquake-stricken area. Clinics. 2015, 70(1):69-72.
• Uotani T, Graham DY. Diagnosis of Helicobacter pylori using the rapid urease test. Annals of Translational Medicine. , 3(1):9. Finegold S.M., Baron E.J. Bailey and scott’s Diagnostic microbiology 7thed. C.V. Mosby Company, St. Louis, Toronto, Princeton. Correa P, Houghton J. Carcinogenesis of Helicobacter pylori. Gastroenterology. 2007, 133(2):659-72.
• Mainmooma M., Habibullah C.M., Singh N. Evaluation of methods to detect H. pylori from human antral mucosa. 1994.
• Indian Journal of Medical Microbiology. 12(1): 39-41. Schnell A.A., Schubert T.T. Usefulness of culture, histology and urease testing in the detection of C. pylori. Am. J. Gastroenterol. 1989, 84: 133-137.
• Hazell, S. L., Lee, A., Brady, L., and Hennessy, W. Campylobacter pyloridis and gastritis: Association with intercellural spaces and adaptation to an environment of mucus as important factors in colonization of gastric epithelium. J Infect Dis. 1986, 153: 658-663.
• McNulty C.A., Dent J.C., Uff J.S., Gear M.W., Wilkinson S.P. Detection of Campylobacter pylori by the biopsy urease test: an assessment in 1445 patients. 1989. Gut, 30: 1058-1062.
• Brown L.M. Helicobacter pylori: epidemiology and routes of transmission. Epidemiol Rev. 2000, 22(2):283-97.