Estimation of glucose level and body weight in Alloxan Induced Diabetic Rat treated with Aqueous extract of Garcinia Kola Seed

Year 2015, Volume: 1 Issue: 2, 26 - 30, 30.01.2016

Chinwe Ewenighi

Abstract

Aim: Hypoglyceamic effect of Garcinia kola (GK) was evaluated in alloxan induced diabetic rats.
Method: Eighteen male wistar albino rats of weights between 158-172g were divided into three groups A- C. Group A was given distilled water (D/W) throughout the experiment; diabetes was induced in group B and C. Group B was given d/w but not GK while group C was treated with GK extract for 21 days. Fasting blood samples were collected at baseline, 24 hours, 7th, 14th and 21st day for blood glucose evaluation.
Results: Mean fasting blood glucose (FBG) level was significantly higher (P<0.001) after 24 hours diabetes induction with alloxan in groups B and C rats compared to the baseline. Group B showed geometric increase in FBG level and decrease in body weight from 24 hours after diabetes induction to the 21st day of study duration. Group C rats had significantly lower (P<0.001) mean FBG level than Group B rats. Significant difference in body weight was observed among the 3 groups throughout the study periods but for baseline.
Conclusion: Treatment of alloxan induced diabetic rats with GK extract lowers blood glucose level and restores weight loss.

Keywords

Garcinia kola, alloxan, glucose, diabetes mellitus, hypoglyceamia, hyperglyceamia

References

• WHO Diabetes Fact sheet N°312". October 2013. Retrieved 23 March 2015.
• Jenson T, Stender DT. Abnormalities in plasma concentration of lipoprotein and fibrinogen in type 1 diabetics with increased urinary albumin excretion. Diabetology 1998; 31:142-145. [DOI via Crossref]
• Mazumder PM, Farswan M, Parcha V. Effect of an isolated active compound (Cg-1) of Cassia glauca leaf on blood glucose, lipid profile and atherogenic index in diabetic rats. Indian J. Pharmacol 2009; 41:182-186. [DOI via Crossref] [Pubmed] [PMC Free Fulltext]
• Adesanya QA, Oluyemi KA et al. Micromorphometric and stereological effects of ethanolic extracts of Garcinia cambogia seeds on the testes and Epididymides of adult Wistar Rats. The Internet Journal of Alternative Medicines 2007; 5(1):22-34.
• Okwu DE. Phytochemical, Vitamins and Mineral contents of two Nigerian medicianal plants. Int J Mol Med and Adv Sci 2005; 1(4):375-381.
• Nzegbule E, Mbakwe R. Effect of pre-sowing and incubation treatment on Germination of Garcinia Kola Heckel seed. Fruita 2001; 56:437-442. [DOI via Crossref]
• Ofor CC, Oguwike FN, Onubueze DPM, Olisa MC. Effects of Bitter Cola (Garcinia Kola) On Haemostatic and Biochemical Indiced Male Diabetic Albino Wistar Rats. IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) 2013; 11(3):53-57. [DOI via Crossref]
• Adaramoye OA. Antidiabetic effect of kolaviron, a biflavonoid complex isolated from Garcinia kola seeds in Wistar rats. Afr Health Sci 2012; 12(4):498-506. [Pubmed] [PMC Free Fulltext]
• Udenze ECC, Braide VB, Okwesilieze CN, Akuodor GC. Pharmacological Effects of Garcinia kola Seed Powder on Blood Sugar, Lipid Profile and Atherogenic Index of Alloxan-induced Diabetes in Rats. Pharmacologia 2012;3(12):693-699. [DOI via Crossref]
• Imo Chinedu et al. Acute administration of aqueous extract of Garcinia kola on daily blood glucose level and selected biochemical indices in longevity wistar albino rats. International Journal of Microbiology and Mycology 2013;1(2):7-12.
• Thomas BO, Ibeabuchi NM et al. Effects of Garcinia kola on glucose metabolism in rats. 9th Scientific Congress of anatomical Society of West Afric 2000, Ibadan, Nigeria.
• Adaramoye OA, Adeyemi EO, Emerole GO (2003) Evaluation of hypoglycemic and anti-hyperglycemic activities of flavonoids from Garcinia kola seeds in rats. 7th Pan Arab conference on Diabetes, Cairo, Egypt.
• Azu OO, Osinubi AA, Noronha CC, Okanlawon AO. Hypoglycaemic Activities of Extract of Garcinia Kola Seeds in Normal, Hyperglycaemic and Alloxan-Induced Diabetic Rats. West Africa Journal of Anatomy 2005;8:141-149.
• Omage Kingsley, Idokpesi O Georginah, Josiah J Sunday et al. Evaluation of hypoglycemic and antioxidant properties of aqueous extract of Garcinia kola seeds in wister rats. Research Journal of Biological Sciences 2010;5(10):647-647. [DOI via Crossref]
• Akbarzadeh A, Norouzian D, Mehrabi MR, Jamshidi SH, Farhangi A, Allah Verdi A, Mofidian SMA, Lame Rad B. Induction of diabetes by streptozotocin in rats. Indian Journal of Clinical Biochemistry 2007;22(2):60-64. [DOI via Crossref] [Pubmed] [PMC Free Fulltext]
• Daye Cheng, Bin Liang, Yunhui Li. Antihyperglycemic Effect of Ginkgo biloba Extract in Streptozotocin-Induced Diabetes in Rats. BioMed Research International Volume 2013; Article ID 162724, 7 pages.
• Muhammad Zafar, Syed Naeem-ul-Hassan Naqv. Effects of STZ-Induced Diabetes on the Relative Weights of Kidney, Liver and Pancreas in Albino Rats: A Comparative Study. Int. J. Morphol 2010;28(1):135-142. [DOI via Crossref]
• Okon UA, Owo DU, Udokang NE, Udobang JA, Ekpenyong CE. Oral Administration of Aqueous Leaf Extract of Ocimum Gratissimum Ameliorates Polyphagia, Polydipsia and Weight Loss in Streptozotocin-Induced Diabetic Rats. American Journal of Medicine and Medical Sciences 2012;2(3): 45-49. [DOI via Crossref]
• WHO. Expert Committee on Diabetes Mellitus, Technical Report Series World Health Organization 1980, Geneva.
• Ewenighi CO, Dimkpa U, Adejumo B I, Onyeanusi JC, Onoh L U M, Ezeugwu U, Onoh G O, Uzor S, Orji E, Anojulu A. Estimation of lipid profile and glucose level in alloxan-induced diabetic rats treated with Cymbopogon citratus (lemongrass). J Exp Integr Med 2013;3(3):249-253. [DOI via Crossref]
• Lenzen S. The mechanisms of alloxan and streptozotocin induced diabetes. Diabetologica 2008;51:216-226. [DOI via Crossref] [Pubmed]