Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls (Boz Irkı ve Holstein Melezi Erkek Sığırlarda IGF1 C472T ve TG C422T Polimorfizmlerinin Analizi)

Öz

Bu çalışmanın amacı, Boz ırkı ve Holstein melezi erkek sığırlarda insulin benzeri büyüme faktörü 1 ve tiroglobulin genlerine ait genotipik dağılımın belirlenmesidir. Çalışmada, akraba olmayan 30 baş Boz ırk ve 26 baş Holstein melez sığır kullanılmıştır. Genomik DNA izolasyonu tam kandan standart fenol-kloroform yöntemi ile yapılmıştır. İnsulin benzeri büyüme faktörü 1 ve tiroglobulin genleri, C472T ve C422T polimorfizmlerine ait genotiplendirme işlemi için ise polimeraz zincir reaksiyonu- restriksiyon parçacık uzunluk polimorfizmi tekniği kullanılmıştır. Hardy-Weinberg eşitliğine uyum PopGene paket programı ile hesaplanmıştır. Bununla birlikte, heterozigotluk, efektif allel sayısı ve polimorfizm bilgi içeriğinden oluşan populasyon genetiği parametreleri allelik dağılım temel alınarak belirlenmiştir. İnsulin benzeri büyüme faktörü 1 geni için, A alleli frekansı iki ırk grubunda da yüksek bulunmuş ve BB genotipi gözlenmemiştir. Tiroglobulin geni için ise, TT genotipi gözlenmemiş, buna ek olarak, C allelinin ise hem Boz ırkta hem de Holstein melezlerinde neredeyse sabit olduğu belirlenmiştir. Bunun yanı sıra, oldukça düşük minör allel frekansları populasyon indekslerindeki düşük varyasyonlara sebep olmuştur. Bu çalışmadan elde edilen sonuçların insulin benzeri büyüme faktörü 1 ve tiroglobulin genleri kapsamında sığır genomunda yapılacak olan sonraki çalışmalara yararlı olabileceği düşünülmektedir.  

Anahtar Kelimeler

• sığır,Boz ırk,Holstein melezi,IGF1,TG,PCR-RFLP

Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls

Öz

The aim of the study was to determine genotypic distribution of bovine insulin-like growth factor 1 and thyroglobulin genes in Turkish Grey Steppe (Boz) and Holstein crossbred bulls. In the study, unrelated 30 Turkish Grey and 26 Holstein crossbred cattle were used. Genomic DNA was isolated from whole blood using standart phenol-chloroform extraction method. The polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping of C472T and C422T polymorphisms in insulin-like growth factor 1 and thyroglobulin genes, respectively. Hardy-Weinberg equilibrium was calculated by using PopGene software program. In addition the population indexes including heterozygosity, number of effective alleles and polymorphism information content were determined on the basis of allelic distribution. For insulin-like growth factor 1 gene, A allele frequency was higher and BB genotype was not present in both breed groups. For thyroglobulin gene, TT genotype was not present and moreover C allele was almost fixed in both Turkish Grey and Holstein crossbred bulls. In addition, extremely low minor allele frequencies in resulted in low genetic variabilities of population indexes. The present results may be useful for future studies conducted on bovine genome with respect to insulin-like growth factor 1 and thyroglobulin genes.    

Anahtar Kelimeler

• cattle,Turkish Grey,Holstein crossbreds,IGF1,TG,PCR-RFLP

Kaynakça

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