Biochemical Differences and Rapid Identification of Yersinia ruckeri and Lactococcus garvieae Isolated from Aquaculture

Öz

            Aquaculture has growing much more than 100% at the last decade. Especially rainbow trout is the most economically important fish in aquatic species, yet the bacterial infections limit freshwater fish production especially Yersinia ruckeri and Lactococcus garvieae. There are many identification reports on the bacterial agent but each study should be supported with detailed biochemical features. Therefore, we aimed of this study, determination of biochemical differences and identify two bacteria in a short time by using rapid test kits. 30 isolates of each Y. ruckeri and L. garvieae used that are represent of different regions of Turkey were used and biochemical characteristics were determined by basic microbiologic test and API RAPID test kits. Y. ruckeri is the most heterogenetic biochemical structure comparing to L. garvieae. All isolates were successfully identified within 5-7 hours by using rapid test kits while there are some differences between each other. The extensive biochemical differences of Y. ruckeri and L. garvieae were shown in the present study.

Anahtar Kelimeler

• Yersinia ruckeri,Lactococcus garvieae,API RAPID Test Kits,biochemical differences

Biochemical Differences and Rapid Identification of Yersinia ruckeri and Lactococcus garvieae Isolated from Aquaculture (Akuakültürden İzole Edilen Y. ruckeri ve L. garvieae İzolatlarının Hızlı Teşhisi ve Biyokimyasal Farklılıklarının Belirlenmesi)

Öz

Akuakültür son on yılda %100’den fazla büyüme göstermiştir. Akuatik balık türleri arasında özellikle gökkuşağı alabalığı en büyük ekonomik öneme sahip türlerdür ancak Yersinia ruckeri ve Lactococcus garvieae gibi bakteriyel enfeksiyonlar akuakültür üretimini sınırlandırmaktadır. Bakteriyel etkenlerin teşhisine yönelik çok sayıda çalışma yapılmış, ancak bakterilerin kesin teşhisinin detaylı biyokimyasal testlerle desteklenmesi gerektiği belirtilmiştir. Bu amaçla, çalışmamızda Y. ruckeri ve L. garvieae etkenlerinin ayrıntılı biyokimyasal farklılıklarının belirlenmesi ve hızlı teşhis kitleri kullanılarak identifikasyonun kısa bir sürede yapılması amaçlanmıştır. Türkiye’nin farklı bölgelerinden izole edilmiş olan Y. ruckeri ve L. garvieae izolatlarından 30’ar izolat incelenmiş, konvansiyonel mikrobiyolojik testler ve API RAPID test kitleri ile biyokimyasal özellikleri belirlenmiştir. Biyokimyasal özellikler açısından Y. ruckeri izolatlarının L. garvieae izolatlarına göre daha heterojen olduğu belirlenmiştir. İzolatlar arasında biyokimyasal farklılık olmasına rağmen, tüm izolatlar hızlı teşhis kitleri kullanılarak 5-7 saat içerisinde başarılı bir şekilde identifiye edilmiştir. Y. ruckeri ve L. garvieae izolatlarının ayrıntılı biyokimyasal özellikleri çalışmamızda sunulmuştur.

Anahtar Kelimeler

• Yersinia ruckeri,Lactococcus garvieae,API hızlı teşhis kiti,biyokimyasal farklılık

Kaynakça

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