Sıçanlara merkezi ve periferik olarak enjekte edilen nesfatin-1'in elektrokardiyografi üzerine etkisi

- , 02.06.2019

Murat Yalçın , Kübra Çiftci , Gökçen Güvenç Ayşenur Baş

https://doi.org/10.30782/uluvfd.445316

Öz

Nesfatin-1 anoreksik nükleobindin-2-türevli bir
peptiddir ve doğrudan ve merkezi olarak kalbe etki eder. Bu çalışma, merkezi ve
periferik yolla uygulanan nesfatin-1'in, 12 saat boyunca aç bırakılmış ve ad
libitum olarak beslenmiş tok sıçanların elektrokardiyografisi (EKG) üzerindeki
etkisini belirlemek için tasarlanmıştır. EKG'yi kaydetmek için elektrotlar
ketamin (50 mg / kg; im) ve ksilazin (20 mg / kg; im) anestezi karışımı altında
sıçanların ekstremitelerine derivasyon II olacak şekilde yerleştirildi.

Merkezi olarak uygulanan farklı dozlarda nesfatin-1
(100 ve 200 pmol; syv) aç ve tok sıçanların EKG dalgalarının amplititütünde bir
değişiklik oluşturmaksızın doza ve zamana bağlı olarak, istatistiksel olarak
anlamlı (p <0.05) T dalgası, Q-T aralığı ve R-R aralığı süresinde artışa
sebep oldu. Benzer şekilde, tok sıçanlara nesfatin-1'in (80 μg / kg; iv)
periferik olarak uygulaması, EKG dalgalarının amplititütünde bir değişiklik
meydana getirmeden istatistiksel olarak anlamlı (p <0.05) olarak T dalgasını,
Q-T ve R-R aralığını uzamıştır. Ayrıca, tok ve aç sıçanlara nesfatin-1’in
serebral yan ventriküle uygulanması ve tok sıçanlara nesfatin-1’in iv enjekte
edilmesi kalp atım hızında istatistiksel olarak anlamlı (p <0.05) azalma oluşturmuştur.

Sonuç olarak,
bulgularımız merkezi ve periferik olarak uygulanan nesfatin-1'in EKG'de T dalgası,
Q-T aralığı ve iki R-dalgası aralığı süresinin gecikmesine neden olduğunu ve böylece
kalp hızında bradikardik etkiye yol açtığını göstermektedir.

Anahtar Kelimeler

Nesfatin-1 , Elektrokardiyografi , İntraserebroventriküler , İntravenöz

Effect of central and peripheral injected nesfatin-1 on electrocardiography in rats

Öz

Nesfatin-1 is an anorexic
nucleobindin-2 -derived peptide and it has directly and centrally effect on the
heart. The current study was designed to determine the effect of centrally and
peripherally administered nesfatin-1 on electrocardiography (ECG) of healthy
both fasted rats for 12 h and satiated rats fed ad libitum. In order to record
ECG, the electrodes were placed limbs of at lead II under ketamine (50 mg/kg;
im) and xylazine (20 mg/kg; im) anesthesia mix.

Centrally administered different
doses of nesfatin-1 (100 and 200 pmol; icv) resulted in dose- and time-dependently
a statistically significant increase (p <0.05) in T wave, Q-T interval, and
R-R interval duration without changing in ECG waves’ amplitude in both satiated
and fasted rats. In similar way, peripheral administration of nesfatin-1 (80 μg/kg;
iv) in satiated rats prolonged statistically significant (p <0.05) T wave, Q-T
interval, and R-R interval without producing a change in ECG waves’ amplitude. Moreover,
icv administered nesfatin-1 in fasted and satiated rats, and iv injected
nesfatin-1 in satiated rats induced statistically significant decrease in heart
rate (p <0.05).

In conclusion, our findings
suggest that centrally and peripherally administrated nesfatin-1 caused a delay
in T wave, Q-T interval and two R-waves interval duration in ECG so that
leading to a bradycardic effect in heart rate.    

Anahtar Kelimeler

Nesfatin-1 , Electrocardiography , Intracerebroventricular , Intravenous

Kaynakça

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