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Detection of Antibiotic Resistance in Staphylococcus aureus Strains Isolated from Various Foods

Year 2012, Volume: 31 Issue: 2, 11 - 16, 01.06.2012

Abstract

This study was performed to determine the antibiotic resistance patterns of 27 Staphylococcus aureus strains, isolated from different foodstuffs, to a range of antibiotics including methicillin, penicillin, ampicillin, cefotaxime, teicoplanin, gentamicin, kanamycin, erythromycin, tetracycline, ciprofloxacin, clindamycin, chloramphenicol, quinupristin/dalfopristin and linezolid. Antibiotic susceptibility testing revealed that 88.9% (24/27) of the strains was resistant to at least one of the antibiotics. Most of S. aureus strains showed the resistance to
penicillin (62.9%) and ampicillin (59.3%). For other antibiotics, the resistance rates were 22.2% for erythromycin, 11.1% for cefotaxime, kanamycin and tetracycline, and 7.4% for methicillin and clindamycin. Moreover, 18 of the strains had resistance to multiple antibiotics. So that, 12 strains exhibited resistance to two antibiotics, four strains to three antibiotics, one strain to four antibiotics, and one strain to six antibiotics. However, all the strains were found to be susceptible to teicoplanin, gentamicin, chloramphenicol and linezolid.

References

  • Byarugaba, D.K., 2004. A view on antimicrobial resistance in developing countries and responsi- ble risk factors. Int. J. Antimicrob. Ag., 24, 105- 110.
  • Clinical and Laboratory Standards Institute (CLSI), 2011. Performance standards for antimi- crobial susceptibility testing; twenty-firstinfor- mational supplement M100-S21 (ISBN 1-56238- 742-1), Wayne, PA, USA.
  • Crago, B., Ferrato, C., Drews, S.J., Svenson, L.W., Tyrrell, G., Louie, M., 2012. Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in food samples associated with foodborne illness in Alberta, Canada from 2007 to 2010. Food Microbiol., 32, 202-205.
  • Ezzeldeen, N.A., Mansour, H.A., Ahmed, A.A., 2011. Phenotypic and molecular identification of Staphylococcus aureus isolated from some Egyp- tian salted fish. World Appl. Sci. J., 15, 1703- 1712.
  • French, G.L., 2005. Clinical impact and rele- vance of antibiotic resistance. Adv. Drug Deliv- ery Rev., 57, 1514-1527.
  • Gharsa, H., Slama, K.B., Lozano, C., Gomez- Sanz, E., Klibi, N., Sallem, R.B., Gomez, P., Za- razaga, M., Boudabous, A., 2012. Prevalence, an- tibiotic resistance, virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia. Vet. Microbiol., 156, 367-373.
  • Graham, J.P., Evans, S., Price, L.B., Silbergeld, E.K., 2009. Fate of antimicrobial-resistant enter- ococci and staphylococci and resistance determi- nants in stored poultry litter. Environ. Res., 109, 682-689.
  • Gündoğan, N., Citak, S., Turan, E., 2006. Slime
  • production, DNase activity and antibiotic re- sistance of Staphylococcus aureus isolated from raw milk, pasteurised milk and ice cream sam- ples. Food Control, 17, 389-392.
  • Hawser, S.P., Bouchillon, S.K., Hoban, D.J., Dowzicky, M., Babinchak, T., 2011. Rising inci- dence of Staphylococcus aureus with reduced susceptibility to vancomycin and susceptibility to antibiotics: a global analysis 2004-2009. Int. J. Antimicrob. Ag., 37, 219- 224.
  • Jones, T.F., Kellum, M.E., Porter, S.S., Bell, M., Schaffner, W., 2002. An outbreak of community- acquired foodborne illness caused by methicillin- resistant Staphylococcus aureus. Emerg. Infect. Dis., 8, 82-84.
  • Kluytmans, J., van Leeuwen, W., Goessens, W., Hollis, R., Messer, S., Herwaldt, L., Bruining, H., Heck, M., Rost, J., van Leeuwen, N., et. al., 1995. Food-initiated outbreak of methicillin- resistant Staphylococcus aureus analyzed by pheno- and genotyping. J. Clin. Microbiol., 33 (5), 1121-1128.
  • Mirzaei, H., Farhoudi, H., Tavassoli, H., Farajli, M., Monadi, A., 2012. Presence and antimicrobi- al susceptibility of methicillin-resistant Staphylo- coccus aureus in raw and pasteurized milk and ice cream in Tabriz by culture and PCR tech- niques. Afr. J. Microbiol. Res., 6, 6224-6229.
  • Normanno, G., Corrente, M., La Salandra, G., Dambrosio, A., Quaglia, N.C., Parisi, A., Greco, G., Bellacicco, A.L., Virgilio, S., Celano, G.V., 2007. Methicillin-resistant Staphylococcus aure- us (MRSA) in foods of animal origin product in Italy. Int. J. Food Microbiol., 117, 219-222.
  • Normanno, G., La Salandra, G., Dambrosio, A., Quaglia, N.C., Corrente, M., Parisi, A., Santa- gada, G., Firinu, A., Crisetti, E., Celano, G.V., 2007. Occurence, characterization and antimicro- bial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. Int. J. Food Microbiol., 115, 290-296.
  • Peles, F., Wagner, M., Varga, L., Hein, I., Rieck, P., Gutser, K., Kereszturi, P., Kardos, G., Turcsanyi, I., Beri, B., Szabo, A., 2007. Charac- terization of Staphylococcus aureus strains iso- lated from bovine milk in Hungary. Int. J. Food Microbiol., 118, 186-193.
  • Pereira, V., Lopes, C., Castro, A., Silva, J., Gibbs, P., Teixeira, P., 2009. Characterization for enterotoxin production, virulence factors, and an- tibiotic susceptibility of Staphylococcus aureus isolates from various foods in Portugal. Food Mi- crobiol., 26, 278- 282.
  • Resch, M., Nagel, V., Hertel, C., 2008. Antibiotic resistance of coagulase-negative staphylococci associated with food and used in starter cultures. Int. J. Food Microbiol., 127, 99-104.
  • Rodriguez-Calleja, J.M., Garcia-Lopez, I., San- tos, J.A., Otero, A., Garcia-Lopez, M.L., 2006. Molecular and phenotypic typing of Staphylococ- cus aureus isolates from rabbit meat. Res. Micro- biol., 157, 496-502.
  • Sasidharan, S., Prema, B., Yoga Latha, L., 2011. Antimicrobial drug resistance of Staphylococcus aureus in dairy products. Asian Pac. J. Tro. Bio- med., 130-132.
  • Shahraz, F., Dadkhah, H., Khaksar, R., Mahmoudzadeh, M., Hosseini, H., Kamran, M., Bourke, P., 2012. Analysis of antibiotic re- sistance patterns and detection of mecA gene in Staphylococcus aureus isolated from packaged hamburger. Meat Sci., 90, 759-763.
  • Siemoni, D., Rizzotti, L., Cocconcelli, P., Gazzo- la, S., Dellaglio, F., Torriani, S., 2008. Antibiotic resistance genes and identification of staphylo- cocci collected from the production chain of swine meat commodities. Food Microbiol., 25, 196-201.
  • Spanu, V., Spanu, C., Virdis, S., Cossu, F., Scarano, C., De Santis, E.P.L., 2012. Virulence factors and genetic variability of Staphylococcus aureus strains isolated from raw sheep’s milk cheese. Int. J. Food Microbiol., 153, 53-57.
  • Umoh, V.J., Odobab, M.B., 1999. Safety and quality evaluation of street foods sold in Zaria, Nigeria. Food Control, 10, 9-14.
  • Vazquez-Sanchez, D., Lopez-Cabo, M., Saa- Ibusquiza, P., Rodriguez-Herrera, J.J., 2012. In- cidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (northwest Spain). Int. J. Food Microbiol., 157, 286-296.

Çeşitli Gıdalardan İzole Edilen Staphylococcus aureus Suşlarında Antibiyotik Dirençliliğinin Belirlenmesi

Year 2012, Volume: 31 Issue: 2, 11 - 16, 01.06.2012

Abstract

Bu çalışma, çeşitli gıda maddelerinden izole edilen 27 adet S. aureus suşunun, metisilin, penisilin, ampisilin, sefotaksim, teikoplanin, gentamisin, kanamisin, eritromisin, tetrasiklin, siprofloksasin, klindamisin, kloramfenikol, kinopristin/dalfopristin ve linezolid gibi antibiyotiklere dirençlilik özelliklerini belirlemek amacıyla gerçekleştirildi. Antibiyotik duyarlılık testi, suşlardan %88.9’unun (24/27) en az bir antibiyotiğe dirençli olduğunu ortaya koydu. S. aureus suşlarının çoğunluğu penisilin (%62.9) ve ampisiline (%59.3) dirençlilik gösterdi. Diğer antibiyotikler bakımından dirençlilik oranları, eritromisin için %22.2, sefotaksim, kanamisin ve tetrasiklin için %11.1, metisilin ve klindamisin için %7.4 idi. Üstelik, 18 suş birden fazla antibiyotiğe karşı dirençliliğe sahipti. Öyleki, suşlardan 12’si iki antibiyotiğe, 4’ü üç antibiyotiğe, biri dört antibiyotiğe ve bir suş da altı antibiyotiğe direnç gösterdi. Bununla birlikte, tüm suşların teikoplanin, gentamisin, kloramfenikol ve linezolid’e duyarlı olduğu bulundu

References

  • Byarugaba, D.K., 2004. A view on antimicrobial resistance in developing countries and responsi- ble risk factors. Int. J. Antimicrob. Ag., 24, 105- 110.
  • Clinical and Laboratory Standards Institute (CLSI), 2011. Performance standards for antimi- crobial susceptibility testing; twenty-firstinfor- mational supplement M100-S21 (ISBN 1-56238- 742-1), Wayne, PA, USA.
  • Crago, B., Ferrato, C., Drews, S.J., Svenson, L.W., Tyrrell, G., Louie, M., 2012. Prevalence of Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) in food samples associated with foodborne illness in Alberta, Canada from 2007 to 2010. Food Microbiol., 32, 202-205.
  • Ezzeldeen, N.A., Mansour, H.A., Ahmed, A.A., 2011. Phenotypic and molecular identification of Staphylococcus aureus isolated from some Egyp- tian salted fish. World Appl. Sci. J., 15, 1703- 1712.
  • French, G.L., 2005. Clinical impact and rele- vance of antibiotic resistance. Adv. Drug Deliv- ery Rev., 57, 1514-1527.
  • Gharsa, H., Slama, K.B., Lozano, C., Gomez- Sanz, E., Klibi, N., Sallem, R.B., Gomez, P., Za- razaga, M., Boudabous, A., 2012. Prevalence, an- tibiotic resistance, virulence traits and genetic lineages of Staphylococcus aureus in healthy sheep in Tunisia. Vet. Microbiol., 156, 367-373.
  • Graham, J.P., Evans, S., Price, L.B., Silbergeld, E.K., 2009. Fate of antimicrobial-resistant enter- ococci and staphylococci and resistance determi- nants in stored poultry litter. Environ. Res., 109, 682-689.
  • Gündoğan, N., Citak, S., Turan, E., 2006. Slime
  • production, DNase activity and antibiotic re- sistance of Staphylococcus aureus isolated from raw milk, pasteurised milk and ice cream sam- ples. Food Control, 17, 389-392.
  • Hawser, S.P., Bouchillon, S.K., Hoban, D.J., Dowzicky, M., Babinchak, T., 2011. Rising inci- dence of Staphylococcus aureus with reduced susceptibility to vancomycin and susceptibility to antibiotics: a global analysis 2004-2009. Int. J. Antimicrob. Ag., 37, 219- 224.
  • Jones, T.F., Kellum, M.E., Porter, S.S., Bell, M., Schaffner, W., 2002. An outbreak of community- acquired foodborne illness caused by methicillin- resistant Staphylococcus aureus. Emerg. Infect. Dis., 8, 82-84.
  • Kluytmans, J., van Leeuwen, W., Goessens, W., Hollis, R., Messer, S., Herwaldt, L., Bruining, H., Heck, M., Rost, J., van Leeuwen, N., et. al., 1995. Food-initiated outbreak of methicillin- resistant Staphylococcus aureus analyzed by pheno- and genotyping. J. Clin. Microbiol., 33 (5), 1121-1128.
  • Mirzaei, H., Farhoudi, H., Tavassoli, H., Farajli, M., Monadi, A., 2012. Presence and antimicrobi- al susceptibility of methicillin-resistant Staphylo- coccus aureus in raw and pasteurized milk and ice cream in Tabriz by culture and PCR tech- niques. Afr. J. Microbiol. Res., 6, 6224-6229.
  • Normanno, G., Corrente, M., La Salandra, G., Dambrosio, A., Quaglia, N.C., Parisi, A., Greco, G., Bellacicco, A.L., Virgilio, S., Celano, G.V., 2007. Methicillin-resistant Staphylococcus aure- us (MRSA) in foods of animal origin product in Italy. Int. J. Food Microbiol., 117, 219-222.
  • Normanno, G., La Salandra, G., Dambrosio, A., Quaglia, N.C., Corrente, M., Parisi, A., Santa- gada, G., Firinu, A., Crisetti, E., Celano, G.V., 2007. Occurence, characterization and antimicro- bial resistance of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. Int. J. Food Microbiol., 115, 290-296.
  • Peles, F., Wagner, M., Varga, L., Hein, I., Rieck, P., Gutser, K., Kereszturi, P., Kardos, G., Turcsanyi, I., Beri, B., Szabo, A., 2007. Charac- terization of Staphylococcus aureus strains iso- lated from bovine milk in Hungary. Int. J. Food Microbiol., 118, 186-193.
  • Pereira, V., Lopes, C., Castro, A., Silva, J., Gibbs, P., Teixeira, P., 2009. Characterization for enterotoxin production, virulence factors, and an- tibiotic susceptibility of Staphylococcus aureus isolates from various foods in Portugal. Food Mi- crobiol., 26, 278- 282.
  • Resch, M., Nagel, V., Hertel, C., 2008. Antibiotic resistance of coagulase-negative staphylococci associated with food and used in starter cultures. Int. J. Food Microbiol., 127, 99-104.
  • Rodriguez-Calleja, J.M., Garcia-Lopez, I., San- tos, J.A., Otero, A., Garcia-Lopez, M.L., 2006. Molecular and phenotypic typing of Staphylococ- cus aureus isolates from rabbit meat. Res. Micro- biol., 157, 496-502.
  • Sasidharan, S., Prema, B., Yoga Latha, L., 2011. Antimicrobial drug resistance of Staphylococcus aureus in dairy products. Asian Pac. J. Tro. Bio- med., 130-132.
  • Shahraz, F., Dadkhah, H., Khaksar, R., Mahmoudzadeh, M., Hosseini, H., Kamran, M., Bourke, P., 2012. Analysis of antibiotic re- sistance patterns and detection of mecA gene in Staphylococcus aureus isolated from packaged hamburger. Meat Sci., 90, 759-763.
  • Siemoni, D., Rizzotti, L., Cocconcelli, P., Gazzo- la, S., Dellaglio, F., Torriani, S., 2008. Antibiotic resistance genes and identification of staphylo- cocci collected from the production chain of swine meat commodities. Food Microbiol., 25, 196-201.
  • Spanu, V., Spanu, C., Virdis, S., Cossu, F., Scarano, C., De Santis, E.P.L., 2012. Virulence factors and genetic variability of Staphylococcus aureus strains isolated from raw sheep’s milk cheese. Int. J. Food Microbiol., 153, 53-57.
  • Umoh, V.J., Odobab, M.B., 1999. Safety and quality evaluation of street foods sold in Zaria, Nigeria. Food Control, 10, 9-14.
  • Vazquez-Sanchez, D., Lopez-Cabo, M., Saa- Ibusquiza, P., Rodriguez-Herrera, J.J., 2012. In- cidence and characterization of Staphylococcus aureus in fishery products marketed in Galicia (northwest Spain). Int. J. Food Microbiol., 157, 286-296.
There are 25 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Figen Çetinkaya This is me

Tülay ELAL Mus This is me

Publication Date June 1, 2012
Published in Issue Year 2012 Volume: 31 Issue: 2

Cite

APA Çetinkaya, F., & Mus, T. E. (2012). Detection of Antibiotic Resistance in Staphylococcus aureus Strains Isolated from Various Foods. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 31(2), 11-16.
AMA Çetinkaya F, Mus TE. Detection of Antibiotic Resistance in Staphylococcus aureus Strains Isolated from Various Foods. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. June 2012;31(2):11-16.
Chicago Çetinkaya, Figen, and Tülay ELAL Mus. “Detection of Antibiotic Resistance in Staphylococcus Aureus Strains Isolated from Various Foods”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31, no. 2 (June 2012): 11-16.
EndNote Çetinkaya F, Mus TE (June 1, 2012) Detection of Antibiotic Resistance in Staphylococcus aureus Strains Isolated from Various Foods. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31 2 11–16.
IEEE F. Çetinkaya and T. E. Mus, “Detection of Antibiotic Resistance in Staphylococcus aureus Strains Isolated from Various Foods”, Uludağ Üniversitesi Veteriner Fakültesi Dergisi, vol. 31, no. 2, pp. 11–16, 2012.
ISNAD Çetinkaya, Figen - Mus, Tülay ELAL. “Detection of Antibiotic Resistance in Staphylococcus Aureus Strains Isolated from Various Foods”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31/2 (June 2012), 11-16.
JAMA Çetinkaya F, Mus TE. Detection of Antibiotic Resistance in Staphylococcus aureus Strains Isolated from Various Foods. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2012;31:11–16.
MLA Çetinkaya, Figen and Tülay ELAL Mus. “Detection of Antibiotic Resistance in Staphylococcus Aureus Strains Isolated from Various Foods”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, vol. 31, no. 2, 2012, pp. 11-16.
Vancouver Çetinkaya F, Mus TE. Detection of Antibiotic Resistance in Staphylococcus aureus Strains Isolated from Various Foods. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2012;31(2):11-6.