Internal Form of Caseous Lymphadenitis in a Merino-Sheep

Year 2012, Volume: 31 Issue: 2, 71 - 76, 01.06.2012

Volkan İpek Ömer Akgül M. Müfit Kahraman Sevda İnan Öztürkoğlu Esra Büyükcangaz Zafer Mecitoğlu Gülnaz YILMAZBAŞ Mecitoğlu

Abstract

Corynebacterium pseudotuberculosis, caseous lymphadenitis, is a chronic disease characterized with internal organ abscess formation and has been reported in many sheep raised countries. The agent is a pleomorphic, gram-positive bacteria and proliferates within the macrophages. Poor fleece quality, milk production decrease, reproductive disorders are among the causes of economical loses since there is no good response to the treatment efforts. A caseous lymphadenitis case in a pregnant two-year-old Merino sheep with respiratory problems evaluated with clinical, microbiological and pathological findings and presented here. Following necropsy, tissue samples processed routinely, stained with hematoxylin-eosin and examined. For the microbiology Gram staining of the samples was made and samples were cultured on GBL blood sheep, BHI, MacConkey agars and CAMP test was applied and C. pseudotuberculosis bacteriologically identified. Examination of mediastinal lymph node showed lamella formation and a pyogranulomatous reaction. A well-demarcated abscess was ob served in the examination of the liver. The right kidney has only a membranoproliferative glomerulonephritis. Along with vaccination, serology has an importance in the detection of infected animals and their culling and is necessary in the complete eradication of caseous lymphadenitis. In that sense, much more importance must be given to the small ruminant vaccination and eradication efforts of the diseases in Turkey in which it has up to 63% prevalence.

Keywords

Corynebacterium pseudotuberculosis, caseous lymphadenitis, mediastinal lymph node, kidney, liver

Merinos Irkı Bir Koyunda İç Organ Tutulumlu Kazeöz Lenfadenitis Olgusu

Abstract

Koyunlarda Corynebacterium pseudotuberculosis, kazeöz lenfadenitis (KLA) ve iç organlarda apselerle karakterize olan, kronik bir hastalıktır ve koyun yetiştirilen birçok ülkede rapor edilmiştir. Etken, pleomorfik, Gram pozitif ve makrofajlarda çoğalan bir bakteridir. Zayıf yün gelişimi, süt veriminde düşme, üremede bozukluklar nedeniyle önemli ekonomik kayıplara neden olmakta ve çoğunlukla tedaviye yanıt alınamamaktadır. Sunulan vakada iki yaşlı, solunum problemi şikâyeti ile getirilen Merinos ırkı bir koyunda gözlenen KLA olgusu klinik, mikrobiyolojik ve patolojik bulgular eşliğinde değerlendirildi. Nekropsi sonrasında alınan organ numunelerine rutin takip işlemleri uygulanarak mikroskobik incelemeler yapıldı. Mikrobiyolojik incelemede Gram boyama, GBL koyun kanlı agarı, BHI ve MacConkey agara ekim ve CAMP testi uygulandı ve C. pseudotuberculosis identifiye edildi. Mediastinal lenf yumrusunun muayenesinde lamella oluşumlu piyogranulomatöz reaksiyon gözlendi. Karaciğerin histopatolojik muayenesinde ise iyi sınırlanmış apse alanları mevcuttu. Sağ böbrekte ise sadece membranoproliferatif bir glomerulonefritis görüldü. Aşılamanın yanı sıra, serolojik tanı hastalıkla savaşmada çok önemli bir yer tutar; böylece belirlenen enfekte hayvanların ayrılması veya itlafı yoluyla hastalıkla mücadele edilebilir ve bu KLA’nın tam eradikasyonu için gerekli görülmektedir. Ülkemizde küçük ruminantlarda yaklaşık %63 oranında görülen bu hastalığa yönelik olarak, aşılama ve eradikasyon çalışmalarına daha fazla ağırlık verilmesi gerekmektedir

Keywords

Corynebacterium pseudotuberculosis, kazeöz lenfadenitis, mediastinal lenf yumrusu, böbrek, karaciğer

References

• Al-Gaabary M.H., Osman S.A., Oreiby A.F., 2009. Caseous lymphadenitis in sheep and goats: Clinical, epidemiological and preventive studies. Small Ruminant Research, 87, 116–121.
• Al-Gaabary M.H., Osman S.A., Ahmed M.S., Oreiby A.F., 2010. Abattoir survey on caseous lymphadenitis in sheep and goats in Tanta, Egypt. Small Ruminant Research, 94, 117–124.
• Baird G.J., and Fontainey M.C., 2007. Coryne- bacterium pseudotuberculosis and its role in ovi- ne caseous lymphadenitis. J. Comp. Path., 137, 179-210.
• Cetinkaya B., Karahana M., Atila E., Kalina R., Baereb T.D., Vaneechoutteb M., 2002. Identifica- tion of Corynebacterium pseudotuberculosis iso- lates from sheep and goats by PCR. Veterinary Microbiology, 88, 75–83.
• Costa M.P., McCulloch J.A., Almeida S.S., Do- rella F.A., Fonseca C.T., Oliveira D.M., Teixeira M.F.S., Laskowska E., Lipinska B., Meyer R., Portela R.W., Oliveira S.C., Miyoshi A., Azeve- do V., 2011. Molecular characterization of the C. pseudotuberculosis hsp60-hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice. BMC Research Notes, 4, 243.
• Dercksen D.P., ter Laak E.A., Schreuder B.E.C., 1996. Eradication programme for caseous lymp- hadenitis in goats in the Netherlands. Veterinary Record, 138, 237.
• Dorella F.A., Pacheco L.G., Seyffert N., Portela R.W., Meyer R., Miyoshi A., Azevedo V., 2009. Antigens of Corynebacterium pseudotuberculosis and prospects for vaccine development. Expert Rev Vaccines, 205, 13.
• Fontaine M.C., Baird G.J., 2008. Caseous lymp- hadenitis. Small Ruminant Research, 76, 42–48.
• Hassan N.A., Al-Humiany A.A., Bahobail A.S., Mansour A.M.A, 2011. Bacteriological and pat- hological studies on caseous lymphadenitis in sheep in Saudi Arabia. İnternational Journal of Microbiological Research, 2, 28-37.
• İlhan F.S., 2008. Koyunların kazeöz lenfadenitis
• enfeksiyonunda patolojik bulgular. Yyü Vet. Fak. Derg., 19, 23-28.
• İzgür M., Akan M., İlhan Z., Yazıcıoğlu N.,
• Studies on vaccine development for ovine caseous lymphadenitis. Ankara Üniv Vet Fak Derg., 57, 161-165.
• Kumar J., Singh F., Tripathi B.N., Kumar R., Dixit S.K., Sonawane G.G., 2012. Epidemiologi- cal, bacteriological and molecular studies on ca- seous lymphadenitis in Sirohi goats of Rajasthan, India. Trop Anim Health Prod., 44, 1319–1322.
• Segal B.H., Wang X.Y., Dennis C.G., Youn R., Repasky E.A., Manjili M.H., Subjeck J.R., 2006. Heat shock proteins as vaccine adjuvants in in- fections and cancer. Drug Discovery Today, 11, 11-12.
• Ural K., Alic D., Haydardedeoglu A.E., Cedden F., Guzel M., Ozyıldız Z., Cantekin Z., 2008. Corynebacterium pseudotuberculosis infection in Saanen×Kilis crossbred (White) goats in Ankara, Turkey and effective kanamycin treatment: A prospective, randomized, double-blinded, place- bo-controlled clinical trial. Small Ruminant Re- search, 77, 84–88.
• Voigt K., Baird G.J., Munro F., Murraya F., Brülisauer F., 2012. Eradication of caseous lymphadenitis under extensive management con- ditions on a Scottish hill farm. Small Ruminant Research, 106, 21– 24.
• Watson P.J. and Preece B.E., 2001. Report on 31 caseous lymphadenitis infected sheep farms in England and Wales. Veterinary Record, 148, 663-665.
• Zavoshti F.R., Khoojine A.B.S., Helan J.A., Hassanzadeh B., Heydari A.A., 2012. Frequency of caseous lymphadenitis (CLA) in sheep slaugh- tered in an abattoir in Tabriz: comparison of bac- terial culture and pathological study. Comp Clin Pathol., 21, 667-671.