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Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis

Year 2012, Volume: 31 Issue: 1, 7 - 10, 01.06.2012

Abstract

Ketosis is a disease of high yielding dairy cows caused by negative energy balance. Subclinical ketosis is a type of disease in which keton bodies in blood are elevated but not enough to cause clinical findings. Economic impact of subclinic ketosis makes disease one of the most important problems of dairy industry. Quick and accurate diagnosis of sublinical ketosis is crucial for economic development of a dairy herd thus in presented study two cowside BHBA test were compared according to two different blood BHBA cutoff values. Sensitivities were found to be 58%, 50% and specifities were 84%, 93% respectively for cut off points of 1.2 and >1.4mmol/L. overall specifity and sensitivity levels calculated indicates that Precision Xtra™ could be used for detection of blood BHBA levels in suspected cows at the same time Precision Xtra™ is quicker than Ketosite analysis takes 20 seconds per sample however with Ketosite one analysis takes about 2 minutes.

References

  • Baird, G.D., 1982. Primary ketosis in the high- producing dairy cow: clinical and subclinical dis- orders, treatment, prevention, and outlook. J Dairy Sci., 65, 1–10.
  • Blood, D.C., Morrıs, R.S., Wıllıamsonn, B., Cannon, C.M., Cannon, R.M.A., 1978. Health program for commercial dairyherds. 1. Objec- tives and methods. Aust Vet J., 54, 207-215.
  • Blood, D.C., Radostits, O.M.,1989. Veterinary Medicine, Bailliere Tindall, Philadelphia, 1128- 1138.
  • Duffield,T.,2000. Subclinical ketosis in lactating dairy cattle. Vet. Clin. North Am. Food Anim. Pract.,16, 231–253.
  • Duffield, T., 2002. Impact, Prevention, and Monitoring of Subclinical Ketosis in Transition Dairy Cows. Proc. Minn. Dairy Health Conf.
  • Duffield, T. F., Lissemore, K.D., McBride, B.W., Leslie, K.E.,2009. Impact of hyperketonemia in early lactation dairy cows on health and produc- tion. J. Dairy Sci., 92, 571–580.
  • Dohoo, I.R., Martin, S.W.,1984. Subclincial ketosis: prevalence and associations with produc- tion and disease. Can. J. Comp. Med., 48, 1-5.
  • Fleming, S.A.,2002. Ketosis of ruminants (Ace- tonemia), Large Animal Internal Medicine, Mos- by Company.,1241–1247.
  • Geishauser , T., Leslie, K., Kelton, D., Duffield, T.,1998. Evaluation of five cowside tests for use with milk todetect subclinical ketosis in dairy cows. J. Dairy Sci., 81:,438–443.
  • Geishauser, T., Leslie, K., Tenhag, J., Bashiri, A.,2000. Evaluation of eight cow-side ketone tests in milk fordetection of subclinical ketosis in dairy cows. J. Dairy Sci., 83, 296–299.
  • Geishauser, T., Leslie, K., Kelton, D., Duffield ,T., 2001. Monitoring for subclinical ketosis in dairy herds.Compend. Cont. Educ. Food Anim., 23, 65–71. 10
  • Gerloff, B. J.,2000. Dry cow management for the prevention of ketosis and fatty liver in dairy cows. Vet. Clin. North Am. Food Anim. Pract., 16, 283–292.
  • Iwersen, M., Falkenberg, U., Voigtsberger, R., Forderung, D.,2009. Heuwiese Evaluation of an electronic cowside test to detect subclinical keto- sis in dairy cows. Journal of Dairy Science., 92(6), 2618–2624.
  • Oetzel, GR.,2004. Monitoring and testing dairy herds for metabolic disease. Vet. Clin. North Am. Food Anim. Pract., 20, 651–674.
  • Panousis, N., Kritsepi, M., Karagiannis, I., Kalaitzakis, E., Laf,i S., Brozos, C.H.,2011. Evaluation of Precision Xceed® for on-site moni- toring of blood β-hydroxybutyric acid and glu- cose in dairy cows. Journal of the Hellenic Veter- inary Medical Society., 62(2), 109-117.
  • Stokol, T., Nydam, D.V.,2006. Effect of hemoly- sis on nonesterified fatty acid and beta- hydroxybutyrate concentrations in bovine blood. J. Vet. Diagn. Invest., 18, 466–469.
  • Tyopponen, J., Kauppinen, K., 1980. The stabil- ity and automatic determination of ketone bodies in blood samples taken in field conditions. Acta Vet. Scand., 21, 55-61.

Subklinik Ketozis’in Tanısı İçin İki BHBA Testinin Karşılaştırılması

Year 2012, Volume: 31 Issue: 1, 7 - 10, 01.06.2012

Abstract

Ketozis yüksek süt verimli ineklerde negative enerji dengesizliğine bağlı meydana gelen bir hastalıktır. Subklinik ketozis, kanda keton cisimciklerinin yükselmesiyle oluşan fakat klinik düzeyde semptom görülmemesiyle karakterize bir hastalıktır. Süt sektörünün en önemli hastalığından biri olan subklinik ketozisin ekonomik etkisi fazladır. Subklinik ketozisin hızlı ve doğru tanısı süt sığırcılığın ekonomik gelişimi için önemlidir, sunulan bu çalışmada iki BHBA testi kandaki iki farklı BHBA sınır değerine gore karşılaştırılmıştır. Sensitivite 1.2 mmol/L ye göre %58, 1.4mmol/L ye gore %50 ve spesifite 1.2 mmol/L ye göre %84, 1.4mmol/L ye gore %93 bulunmuştur. Hesaplanan spesifite ve sensivite değerleri gösteriyorki Precision Xtra™ şüpheli sığırların kanlarındaki BHBA düzeylerinin ölçümü için kullanılabilir aynı zamanda Precision Xtra™, Ketosite dan daha hızlıdır çünkü Precision Xtra™ analizi 10 saniyede Ketosite 2 dakikada analizi yapmaktadır

References

  • Baird, G.D., 1982. Primary ketosis in the high- producing dairy cow: clinical and subclinical dis- orders, treatment, prevention, and outlook. J Dairy Sci., 65, 1–10.
  • Blood, D.C., Morrıs, R.S., Wıllıamsonn, B., Cannon, C.M., Cannon, R.M.A., 1978. Health program for commercial dairyherds. 1. Objec- tives and methods. Aust Vet J., 54, 207-215.
  • Blood, D.C., Radostits, O.M.,1989. Veterinary Medicine, Bailliere Tindall, Philadelphia, 1128- 1138.
  • Duffield,T.,2000. Subclinical ketosis in lactating dairy cattle. Vet. Clin. North Am. Food Anim. Pract.,16, 231–253.
  • Duffield, T., 2002. Impact, Prevention, and Monitoring of Subclinical Ketosis in Transition Dairy Cows. Proc. Minn. Dairy Health Conf.
  • Duffield, T. F., Lissemore, K.D., McBride, B.W., Leslie, K.E.,2009. Impact of hyperketonemia in early lactation dairy cows on health and produc- tion. J. Dairy Sci., 92, 571–580.
  • Dohoo, I.R., Martin, S.W.,1984. Subclincial ketosis: prevalence and associations with produc- tion and disease. Can. J. Comp. Med., 48, 1-5.
  • Fleming, S.A.,2002. Ketosis of ruminants (Ace- tonemia), Large Animal Internal Medicine, Mos- by Company.,1241–1247.
  • Geishauser , T., Leslie, K., Kelton, D., Duffield, T.,1998. Evaluation of five cowside tests for use with milk todetect subclinical ketosis in dairy cows. J. Dairy Sci., 81:,438–443.
  • Geishauser, T., Leslie, K., Tenhag, J., Bashiri, A.,2000. Evaluation of eight cow-side ketone tests in milk fordetection of subclinical ketosis in dairy cows. J. Dairy Sci., 83, 296–299.
  • Geishauser, T., Leslie, K., Kelton, D., Duffield ,T., 2001. Monitoring for subclinical ketosis in dairy herds.Compend. Cont. Educ. Food Anim., 23, 65–71. 10
  • Gerloff, B. J.,2000. Dry cow management for the prevention of ketosis and fatty liver in dairy cows. Vet. Clin. North Am. Food Anim. Pract., 16, 283–292.
  • Iwersen, M., Falkenberg, U., Voigtsberger, R., Forderung, D.,2009. Heuwiese Evaluation of an electronic cowside test to detect subclinical keto- sis in dairy cows. Journal of Dairy Science., 92(6), 2618–2624.
  • Oetzel, GR.,2004. Monitoring and testing dairy herds for metabolic disease. Vet. Clin. North Am. Food Anim. Pract., 20, 651–674.
  • Panousis, N., Kritsepi, M., Karagiannis, I., Kalaitzakis, E., Laf,i S., Brozos, C.H.,2011. Evaluation of Precision Xceed® for on-site moni- toring of blood β-hydroxybutyric acid and glu- cose in dairy cows. Journal of the Hellenic Veter- inary Medical Society., 62(2), 109-117.
  • Stokol, T., Nydam, D.V.,2006. Effect of hemoly- sis on nonesterified fatty acid and beta- hydroxybutyrate concentrations in bovine blood. J. Vet. Diagn. Invest., 18, 466–469.
  • Tyopponen, J., Kauppinen, K., 1980. The stabil- ity and automatic determination of ketone bodies in blood samples taken in field conditions. Acta Vet. Scand., 21, 55-61.
There are 17 citations in total.

Details

Primary Language English
Journal Section Articles
Authors

Gülşah Demir This is me

Zafer Mecitoğlu This is me

Serkan Çatik This is me

Sezgin Şentürk This is me

Publication Date June 1, 2012
Published in Issue Year 2012 Volume: 31 Issue: 1

Cite

APA Demir, G., Mecitoğlu, Z., Çatik, S., Şentürk, S. (2012). Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 31(1), 7-10.
AMA Demir G, Mecitoğlu Z, Çatik S, Şentürk S. Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. June 2012;31(1):7-10.
Chicago Demir, Gülşah, Zafer Mecitoğlu, Serkan Çatik, and Sezgin Şentürk. “Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31, no. 1 (June 2012): 7-10.
EndNote Demir G, Mecitoğlu Z, Çatik S, Şentürk S (June 1, 2012) Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31 1 7–10.
IEEE G. Demir, Z. Mecitoğlu, S. Çatik, and S. Şentürk, “Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis”, Uludağ Üniversitesi Veteriner Fakültesi Dergisi, vol. 31, no. 1, pp. 7–10, 2012.
ISNAD Demir, Gülşah et al. “Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 31/1 (June 2012), 7-10.
JAMA Demir G, Mecitoğlu Z, Çatik S, Şentürk S. Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2012;31:7–10.
MLA Demir, Gülşah et al. “Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, vol. 31, no. 1, 2012, pp. 7-10.
Vancouver Demir G, Mecitoğlu Z, Çatik S, Şentürk S. Comparison of Two Cow Side BHBA Tests For Diagnosis of Subclinical Ketosis. Uludağ Üniversitesi Veteriner Fakültesi Dergisi. 2012;31(1):7-10.