Comparison of Estrus and Pregnancy Rates in Cyclic and Non cyclic Anestrus Dairy Cows Treated with Ovsynch, PRID + PGF2α +PMSG and Ear Implant Containing Norgestomet + PGF2α + PMSG

Year 2005, Volume: 24 Issue: 1-2-3-4, 33 - 39, 01.06.2005

Yavuz Nak Deniz Nak Kamil Seyrek İntaş Hasan Basri Tek Abdülkadir Keskin Bilginer Tuna

Abstract

The objective of this study was to compare the effects on estrus detection and first service pregnancy rates, distribution of induced estrus using three differents treatment methods as PRID + PGF2α + PMSG, ear implant containing norgestomet + PGF2α + PMSG, ovsynch in postparum unobserved estrus dairy cows . On 25 dairy farms, 331 unobserved estrus dairy cows being at least on days 60 following parturation randomly assigned to three treatments groups. Group I. On day 0 PRIDs were inserted, on days 7 PGF2α received via im, on days 9 PRIDs removed and 500 IU PMSG injected.and AI occurred approximality at 56th hours following PRIDs removel Group II. Subcutanaus ear implants containing norgestomet were inserted (on day 0), followed immediately administrated norgestomet + estradiol valerate injectabl solution via im. After 7 days, PGF2α was administrated via im, on days 9 implants removed and 500 IU PMSG injected. AI was administrated at approximality 56th hours following implants removel. Group III. On 0 day a GnRH analogue received via im, on 7 days PGF2α administrated, 48th hours later second GnRH injected and AI occured about 16 hours later after second GnRH injection. Cows were observed for sings estrus, twice daily for 5 days. Proportion of cows observed in estrus were 60.36 %, 50.45 %, 22.93 % for groups I, II, III respectively. Estrus detection rates in group I and group II were significantly higher than its group III (ovsynch) (P< 0.001). First service pregnancy rates were determined 39.63 %, 45.94 %, 42.20 % for groups I, II, III respectively. Observed estrus in ovsynch group accumulated in first day, in other groups accumulated in second day after latest hormonal administrations. 

Keywords

PRID, ovsynch, norgestomet, estrus, pregnancy, cyclic anöstrus, noncyclic anöstrus, cattle

Ovsynch, PRD + PGF2α + PMSG ve Norgestomet İçeren Kulak İmplantı + PGF2α + PMSG İle Sağıtılan Siklik ve Asiklik Anöstrüslü Sütçü İneklerde Kızgınlık ve Gebelik Oranlarının Karşılaştırılması

Abstract

Bu çalışmanın amacı, doğum sonrası kızgınlıkları gözlenemeyen sütçü ineklerde PRID + PGF2α + PMSG, Norgestomet içeren kulak implantı + PGF2α + PMSG, Ovsynch olmak üzere üç farklı metodun östrüs tespit ve gebelik oranları, uyarılmış kızgınlıkların dağılımı üzerine etkilerini karşılaştırmaktır. Yirmi beş sütçü işletmede bulunan doğumunun üzerinden en az 60 gün geçmesine rağmen östrüsleri gözlenememiş 331 sütçü inek rasgele olarak uygulama gruplarına dağıtıldı. I. Grup; PRID’ler yerleştirildi (0. gün), 7. günde PGF2α uygulandı, 9'uncu günde PRID’ler çıkarıldı ve 500 IU PMSG im olarak uygulandı. PRID’lerin çıkarılmasını izleyen yaklaşık 56. saatte sun’i tohumlamalar yapıldı. II. Grup; Sıfırıncı günde norgestomet içeren kulak implantları yerleştirildi ve aynı anda norgestomet ve oestradiol valerate içeren enjektabl solüsyon ve 7 gün sonra PGF2α im olarak uygulandı. Dokuzuncu günde implantlar çıkarıldı ve 500 IU PMSG im olarak enjekte edildi. Kulak implantlarının uzaklaştırılmasını izleyen yaklaşık 56. saatte sun’i tohumlamalar yapıldı. III. Grup; Sıfırıncı günde GnRH, 7. günde PGF2α, 9'uncu günde GnRH im olarak uygulandı. Sun’i tohumlamalar son GnRH uygulamasını izleyen yaklaşık 16. saatte yapıldı. Östrüsler hormonal uygulamaların bitiminden itibaren 5 gün süreyle günde 2 kez takip edildi. Östrüs tespit oranları Grup I, Grup II, Grup III’de sırasıyla % 60.36, % 50.45, % 22.93 olarak belirlendi. Östrüs tespit oranlarının Grup I ve Grup II’de, Grup III’e nazaran istatistiksel açıdan önemli olacak düzeyde yüksek olduğu görüldü. İlk servis gebelik oranları Grup I, Grup II ve Grup III’de sırasıyla % 39.63, % 45.94, %42.20 olarak belirlendi. Ovsynch grubunda gözlenebilen östruslar hormonal uygulamaların bitimini izleyen ilk 24 saat içerisinde, diğer gruplarda ise ikinci günde yoğunlaştı

Keywords

PRID, ovsynch, norgestomet, östrüs, gebelik, siklik anöstrüs, asiklik anöstrüs, inek

References

• ALAÇAM E. İnekte infertilite sorunu. Alınmıştır: ALAÇAM E, ed. Evcil Hayvanlarda Doğum ve İnfertilite. Dördüncü Baskı. Ankara: Medisan yayı- nevi. 267 – 288, 2002.
• BRIDGES, PJ, LEWIS, PE, WAGNER, WR, INSKEEP, EK. Folliküler growth, estrus and pregnancy after fıxed-tıme insemination ın beef cows treated wıth ıntravaginal progesteron inserts and estradiol benzoate. Theriogenelogy 1999; 52: 573-589.
• CHUPIN, P, PELOT, J. High fertility in dairy cows after two AI, at oestrus induced by progesteron, prostaglandin and PMSG. reports and summeries patr2, 11th int. congr. On diseases of cattle, Tel- Avivİsrael Association for Buitrics 1980; 1034- 1039.
• DRINCOURT, MARegulation ovarian folliküler dynamics in farm animals, implications for manipulation of reproduction. Theriogenelogy 2001; 55: 1211-1239.
• FERRY, JClinacal management of anestrus. In YOUNQUİST RS, ed. Current Theraphy in Large Animal Theriogenelogy. 1th ed. Philadelphia: W.B.Saunders Company.285-289, 1997.
• FIKE, KE, DAY, ML, INSKEEP, EK, KINDER, JE, LEWIS, PE, SHORT, RE, HAFS, HDEstrus and luteal function in suckled beef cows that were anestrus when treated with an intravaginaldevice containing progesteron with or without a subsequent injection of estradiol benzoate. J.Animal Sci. 1997; 75: 2009-2015.
• HIXON, DL, KESLER, DJ, TROXEL, TR, VINCENT, DL, WİESMWN, BS. Reproductive hormone secratıon and first service conception rate subsequent to ovulation control wıth synchro-mate B. Theriogenology 1981; 16: 219-229.
• KEITH, BR, LESLIE, KE, JOHNSON, WH, WALTON, JSEffect of presynchronization using prostoglandin F2α and a milk – ejection test on pregnancy rate after the timed artificial insemination protocol, ovsynh. Theriogeology 2005; 63: 722-738.
• KIM, UH, SUH, HS, NAM, HW, KANG, HG, KIM, IH. Follicular wave emergence, luteal functions and synchrony of ovulation following GnRH or estradiol benzoate in CIDR- treated lactating holstein cows. Theriogeology 2005; 63: 260-268.
• LAMOGLİA, B., SHORT, RE, BELLOWS, SE, BELLOWS, RS, MACNEİL, MD, HAFS, DA. Induced and synchronized estrus in cattle: Dose titration of estradiol benzoate in peripubertal heifers and postpartum cows after treatment with an intravaginal progesteron-releasing insert and prostaglandin F2α.. J.Animal Sci. 1998; 76: 1662- 1670.
• LUCY, MC, SAVİO, JO, BADINGA, L, SOTA, DL, THATCHER,WW. Factors that affect ovarian follicular dynamics in cattle. J.Anim.Sci. 1992; 70: 3615-3626.
• MİALOT, JP, LAUMONNİER, G, FAUXPOİNT, H, BARASSİN, E, PONTER, AA, DELETANG, F. Postpartum subestrus dairy cows: Comparıson of treatment wıth prostoglandın F2α + GnRH. Theriognelogy 1999; 52: 901-911.
• MİHM, M, BAGISI, M, BOLAND, MP, ROCHE, JFAssociation between the duration of dominance of ovulatory follicle and pregnancy rate in beef heifers. J.Reprod.Fertil. 1994; 102: 123-130.
• MOMCİLOVİC, D, ARCBALD, LF, WALTERS, A, TRAN, T, KELBERT, D, RİSCO, C, THATCHER, WW. Reproductive performance of lactating dairy cows treated with gonadotropin releasing hormone (GnRH) and / or prostoglandin F2α (PGF2α) for synchronization of estrus and ovulation. Theriogenelogy 1998; 50: 1131-1139
• MUNRO, RK. Calving rates of Brahman and Brahman cross cows to fixe-timed insemination after treatment with pregnant mare serum gonadotropin and intravaginal progesteron. Aust. Vet. J. 1988; 65: 21-23.
• NAK, Y. Postpartum anöstrusta teşhis ve tedavi. Doktora Tezi. U.Ü.Sağlık Bilimleri Enstitüsü, Bur- sa, 1984.
• NEBEL, RL, JOBST, SM. Evaluation of systematic breeding programs for lactating dairy cows: Areview. J.Dairy Sci. 1998; 81: 1169-1174.
• ODDE, KG. A revıew of synchronization of in postpartum cattle.; J. Animal Sci. 1990; 68: 817- 830.
• PARKİNSON, TInfertility in cow : structural and functional abnormalities, management deficiencies and non-specific infections. In: NOAKES, DE, PARKİNSON, TJ, ENGLAND, GCW, ARTHUR, GF, eds. 8th ed. Arthur’s Veterinary Reproduction and Obstetrics. London: WB Saunders Company. 383-472, 2001.
• PURSLEY, JR, ME, M.O., WİLTBANK, M.C. Synchronization of ovulation in dairy cattle using PGF2α and GnRH. Theriogenelogy 1995; 44: 915- 923.
• RHODES, FM, BURKE, CR, CLARK, BA, DAY, ML, MACMİLLAN, KL. Effect of treatment with progesteron and oestradiol benzoate on ovarian follicular turnover in postpartum anöstrous cows and cows wich have resumed oestrous cycle. Anim Rep. Sci. 2002; 69: 139-150.
• RHODES, FM, MCDOUGALL, S, BURKE, CR, VERKERK, GA, MACMİLLAN, KL. Invıted reviev: Treatment of with an extended postpartum anestrus interval. J.Dairy Sci. 2003; 86: 1876-1894.
• RYAN, DP, SNİJDERS, H, YAKUUP, H, O’FARREL, KJ. An evaluation of estrus synchronization management of dairy herds. J.Anim. Sci. 1995; 73: 3687-3695. in reproductive
• XU, ZZ, BURTON, LJ. Estrus synchronization of lactating dairy cows with GnRH, progesteron, and prostoglandin F2α. J.Dairy Sci. 2000; 83: 471-476.
• SAVİO, JD, THATCHER, L, BADİNGA, RL, DE LA SOTA, RL, WOLFENSAN, D. Regulation of dominanat follicle turnover during the oestrus cycle in cows. J.Reprod.Fertil. 1993; 97: 197-203.
• SİNG, U, KHURANA, NK, INDERJET, NK. Plasma progeteron profiles and fertility status of anestrus zebu cattle treated wıth norgestomet- estradıol – eCG regimen. Theriogenelogy 1998; 50: 1191-1199.
• SİROUS, J, FORTUNE, JE. Lengtheing the bovine estrus cycle witth low levels of exogeneous progesterone: A model for studying ovarian follicular dominance. Endocrinology 1990; 127: 916-925.
• STEVENSON, JS, KOBAYASHİ, Y, THOMPSON, KE. Reproductive performance of dairy cows in various progrommed breeding system including ovsynch and combination of gonadotropin prostoglandin F2α.. J.Dairy Sci. 1999; 82: 506-515. hormone and
• STEVENSON, JS, THOMPSON, KE, FORBES, WL, LAMB, GC, GREİGER, DM, CORAH, LR. Synchronizing estrus and(or) ovulation in beeff cows after combination of GnRH, norgestomet, and prostoglandin F2α. with or without timed insemination. J. Anim. Sci. 2000; 78: 1747-1758
• ŞENDAĞ, S, ÇELİK, HA, AYDIN, İ, ÇOLAK, M, ÜMÜTLÜ, S. Hakiki anöstrüslü ve suböstrüslü dü- ve ve ineklerin progesteron releasing intravaginal device (PRID) ile tedavisi. Veteriner Bilimleri Dergisi. 2001; 17(3): 129 – 134. 31. THATCHER, WW, MACMİLLAN, KL, HANSEN, PJ, PROST, M. Concepts for regulation of corpus luteum function by tehe conceptus and ovarian Theriognelogy 1989; 31: 149-164. improve fertility.
• ÜNAL, EF, GÖKÇEN, H, NAK, Y, TÜMEN, H. PRID (Progesteron Salan Vagina İçi Alet) kullanı- mı ile inek ve düvelerde anöstrusun sağıtımı. U.Ü.Vet. Fak. Derg. 1992; 11(2): 91-100.
• WİLTBANK, MC, GUMEN, A, SAROTİ, R.
• classification Physiological
• condition in cattle. Theriogenelogy 2002; 57: 21- 52. of anovulatory