Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls (Boz Irkı ve Holstein Melezi Erkek Sığırlarda IGF1 C472T ve TG C422T Polimorfizmlerinin Analizi)

Year 2018, Volume: 37 Issue: 1, 21 - 28, 01.06.2018

Sena Ardicli

https://doi.org/10.30782/uluvfd.392081

Abstract

Bu çalışmanın
amacı, Boz ırkı ve Holstein melezi erkek sığırlarda insulin benzeri büyüme
faktörü 1 ve tiroglobulin genlerine ait genotipik dağılımın belirlenmesidir.
Çalışmada, akraba olmayan 30 baş Boz ırk ve 26 baş Holstein melez sığır
kullanılmıştır. Genomik DNA izolasyonu tam kandan standart fenol-kloroform
yöntemi ile yapılmıştır. İnsulin benzeri büyüme faktörü 1 ve tiroglobulin genleri,
C472T ve C422T polimorfizmlerine ait genotiplendirme işlemi için ise polimeraz
zincir reaksiyonu- restriksiyon parçacık uzunluk polimorfizmi tekniği
kullanılmıştır. Hardy-Weinberg eşitliğine uyum PopGene paket programı ile hesaplanmıştır.
Bununla birlikte, heterozigotluk, efektif allel sayısı ve polimorfizm bilgi
içeriğinden oluşan populasyon genetiği parametreleri allelik dağılım temel
alınarak belirlenmiştir. İnsulin benzeri büyüme faktörü 1 geni için, A alleli frekansı
iki ırk grubunda da yüksek bulunmuş ve BB genotipi gözlenmemiştir. Tiroglobulin
geni için ise, TT genotipi gözlenmemiş, buna ek olarak, C allelinin ise hem Boz
ırkta hem de Holstein melezlerinde neredeyse sabit olduğu belirlenmiştir. Bunun
yanı sıra, oldukça düşük minör allel frekansları populasyon indekslerindeki
düşük varyasyonlara sebep olmuştur. Bu çalışmadan elde edilen sonuçların insulin
benzeri büyüme faktörü 1 ve tiroglobulin genleri kapsamında sığır genomunda
yapılacak olan sonraki çalışmalara yararlı olabileceği düşünülmektedir.  

Keywords

sığır, Boz ırk, Holstein melezi, IGF1, TG, PCR-RFLP

Analysis of IGF1 C472T and TG C422T polymorphisms in Turkish Grey Steppe and Holstein Crossbred Bulls

Abstract

The aim of the study was to determine genotypic distribution of bovine
insulin-like growth factor 1 and thyroglobulin genes in Turkish Grey Steppe (Boz)
and Holstein crossbred bulls. In the study, unrelated 30 Turkish Grey and 26 Holstein
crossbred cattle were used. Genomic DNA was isolated from whole blood using
standart phenol-chloroform
extraction method. The polymerase chain reaction-restriction fragment length polymorphism
technique was used for genotyping of C472T and C422T polymorphisms in insulin-like
growth factor 1 and thyroglobulin genes, respectively. Hardy-Weinberg
equilibrium was calculated by using PopGene software program. In addition the
population indexes including heterozygosity, number of effective alleles and
polymorphism information content were determined on the basis of allelic
distribution. For insulin-like growth factor 1 gene, A allele frequency was
higher and BB genotype was not present in both breed groups. For thyroglobulin gene,
TT genotype was not present and moreover C allele was almost fixed in both
Turkish Grey and Holstein crossbred bulls. In addition, extremely low minor
allele frequencies in resulted in low genetic variabilities of population indexes.
The present results may be useful for future studies conducted on bovine genome
with respect to insulin-like growth factor 1 and thyroglobulin genes.    

Keywords

cattle, Turkish Grey, Holstein crossbreds, IGF1, TG, PCR-RFLP

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