Biochemical Differences and Rapid Identification of Yersinia ruckeri and Lactococcus garvieae Isolated from Aquaculture

Year 2018, Volume: 37 Issue: 1, 37 - 47, 01.06.2018

Muhammed Duman İzzet Burcin Satıcıoglu Soner Altun

https://doi.org/10.30782/uluvfd.399275

Abstract

            Aquaculture
has growing much more than 100% at the last decade. Especially rainbow trout is
the most economically important fish in aquatic species, yet the bacterial
infections limit freshwater fish production especially Yersinia ruckeri and Lactococcus
garvieae. There are many identification reports on the bacterial agent but
each study should be supported with detailed biochemical features. Therefore,
we aimed of this study, determination of biochemical differences and identify
two bacteria in a short time by using rapid test kits. 30 isolates of each Y. ruckeri and L. garvieae used that are represent of different regions of Turkey
were used and biochemical characteristics were determined by basic
microbiologic test and API RAPID test kits. Y.
ruckeri is the most heterogenetic biochemical structure comparing to L. garvieae. All isolates were
successfully identified within 5-7 hours by using rapid test kits while there
are some differences between each other. The extensive biochemical differences
of Y. ruckeri and L. garvieae were shown in the present
study.

Keywords

Yersinia ruckeri, Lactococcus garvieae, API RAPID Test Kits, biochemical differences

Biochemical Differences and Rapid Identification of Yersinia ruckeri and Lactococcus garvieae Isolated from Aquaculture (Akuakültürden İzole Edilen Y. ruckeri ve L. garvieae İzolatlarının Hızlı Teşhisi ve Biyokimyasal Farklılıklarının Belirlenmesi)

Abstract

Akuakültür son on
yılda %100’den fazla büyüme göstermiştir. Akuatik balık türleri arasında
özellikle gökkuşağı alabalığı en büyük ekonomik öneme sahip türlerdür ancak Yersinia ruckeri ve Lactococcus
garvieae
gibi bakteriyel
enfeksiyonlar akuakültür üretimini sınırlandırmaktadır. Bakteriyel etkenlerin
teşhisine yönelik çok sayıda çalışma yapılmış, ancak bakterilerin kesin teşhisinin
detaylı biyokimyasal testlerle desteklenmesi gerektiği belirtilmiştir. Bu
amaçla, çalışmamızda Y. ruckeri ve L. garvieae etkenlerinin ayrıntılı
biyokimyasal farklılıklarının belirlenmesi ve hızlı teşhis kitleri kullanılarak
identifikasyonun kısa bir sürede yapılması amaçlanmıştır. Türkiye’nin farklı
bölgelerinden izole edilmiş olan Y.
ruckeri ve L. garvieae izolatlarından
30’ar izolat incelenmiş, konvansiyonel mikrobiyolojik testler ve API RAPID test
kitleri ile biyokimyasal özellikleri belirlenmiştir. Biyokimyasal özellikler
açısından Y. ruckeri izolatlarının L. garvieae izolatlarına göre daha
heterojen olduğu belirlenmiştir. İzolatlar arasında biyokimyasal farklılık
olmasına rağmen, tüm izolatlar hızlı teşhis kitleri kullanılarak 5-7 saat
içerisinde başarılı bir şekilde identifiye edilmiştir. Y. ruckeri ve L. garvieae
izolatlarının ayrıntılı biyokimyasal özellikleri çalışmamızda sunulmuştur.

Keywords

Yersinia ruckeri, Lactococcus garvieae, API hızlı teşhis kiti, biyokimyasal farklılık

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