Determination of Antioxidant and Some Biochemical Properties of Different Extracts of Tribulus Terrestris

Yıl 2024, Cilt: 4 Sayı: 1, 694 - 708, 25.04.2024

Nazrın Navrozlu Müslüm Kuzu

Öz

Objective: This study determined the antioxidant and total phenolic substance amounts of different extracts (petroleum ether, chloroform, methanol, ethanol) obtained from the iron Tribulus terrestris plant. Methods: Antioxidant activities of the extracts were examined using CUPRAC, FRAP, DPPH, and ABTS methods. In addition, its effects on acetylcholine esterase (AChE) and butyrylcholine esterase (BChE) activities were evaluated in vitro. Results: According to the findings, methanol extract had the highest total phenolic substance amount (63.59±4.33 μg gallic acid/mg extract). In terms of CUPRAC reducing capacity, it was determined that methanol extract had the most substantial effect among the extracts, although it was lower than standard antioxidants. According to the FRAP method, methanol extract had the highest reduction capacity with a 1.47 μmol Fe2+/mg extract value. Similar results were seen with radical scavenging effects. It was the methanol extract with the highest radical scavenging capacity, according to DPPH and ABTS methods. It showed this effect with an IC50 value of 24.39 μg/mL according to the DPPH method and an extract value of 0.47 μg/μg according to the ABTS method. When the enzyme inhibition results were examined, it was seen that the methanol extract had the most potent inhibition effect with an IC50 value of 17.29 μg/mL for the AChE enzyme and 18.23 μg/mL for BChE. Conclusions: According to the results obtained, evidence was obtained that the extracts obtained from the iron thistle plant have an antioxidant effect and may be an option in the treatment of Alzheimer's disease with their inhibition effect on AChE and BChE enzyme activities.

Anahtar Kelimeler

Antioxidant, Acetylcholine esterase, Butyrylcholine esterase, Tribulus terrestris

Proje Numarası

KBÜBAP-22-YL-016

Tribulus Terrestris Bitkisinin Farklı Ekstraktlarının Antioksidan ve Bazı Biyokimyasal Özelliklerinin Belirlenmesi

Öz

Amaç: Bu çalışmada demir Tribulus terrestris (demir diken) bitkisinden elde edilen farklı ekstraktların (petrol eteri, kloroform, metanol, etanol) antioksidan ve toplam fenolik madde miktarını belirlendi. Gereç ve Yöntem: Ekstraktların antioksidan aktiviteleri CUPRAC, FRAP, DPPH ve ABTS yöntemleriyle incelendi. Bunun yanında asetilkolin esteraz (AChE) ve bütirilkolin esteraz (BChE) aktiviteleri üzerine etkileri in vitro olarak değerlendirildi. Bulgular: Elde edilen bulgulara göre toplam fenolik madde miktarı en fazla olan ekstraktın metanol ekstraktı olduğu görüldü (63,59±4,33 μg gallik asit/mg ekstrakt). CUPRAC indirgeme kapasitesi bakımından standart antioksidanlara kıyasla düşük olmakla birlikte ekstraktlar arasında en güçlü etkiye metanol ekstraktının olduğu belirlendi. FRAP yöntemine göre yine metanol ekstraktı 1,47 μmol Fe2+/mg ekstrakt değeri ile en yüksek indirgeme kapasitesine sahipti. Benzer sonuçlar radikal giderme etkilerinde de görüldü. Hem DPPH hem de ABTS yöntemlerine göre radikal giderme kapasitesi en yüksek olan metanol ekstraktı idi. DPPH yöntemine göre 24,39 μg/mL IC50 değeri ile, ABTS yöntemine göre ise 0,47 μg/ μg ekstrakt değerleri ile bu etkiyi gösterdi. Enzim inhibisyon sonuçlarına bakıldığında AChE enzimi için 17,29 μg/mL, BChE için 18,23 μg/mL IC50 değeri ile en güçlü inhibisyon etkisinin yine metanol ekstraktına sahip olduğu görüldü. Sonuç: Elde edilen sonuçlara göre demir diken bitkisinden elde edilen ekstraktların antioksidan etki gösterdiği, AChE ve BChE enzim aktiviteleri üzerine gösterdiği inhibisyon etkisi ile Alzheimer hastalığı tedavisinde bir seçenek olabileceği noktasında ön bilgiler elde edilmiş oldu.

Anahtar Kelimeler

Antioksidan, Asetilkolin esteraz, Bütirilkolin esteraz, Tribulus terrestris

Destekleyen Kurum

Karabük Üniversitesi

Proje Numarası

KBÜBAP-22-YL-016

Teşekkür

Yapılan çalışma Karabük Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından KBÜBAP-22-YL-016 numaralı proje kapsamında desteklenmiştir.

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