Cell Separation with Aqueous Multiphase Systems

Abstract

This study investigates the use of multiphase systems for density-based separation of cervical cancer HeLa cells that contain nanoparticles. Aqueous multiphase systems (MuPSs) are prepared by mixing the various combinations of water-soluble polymers and/or surfactants followed by phase separation. The phases of the system are sorted according to their density and in between neighboring phases there exists an interface that can act as a step-in-density. This interface is also thermodynamically stable since it is formed through phase separation. Subsequent to preparation and optimization of these systems, cells are added to the systems and separation of cells is achieved through centrifugation. In this work, the separation of HeLa cells that interacted with gold nanoparticles, hence experienced a change in their density, was demonstrated.

Keywords

• Multiphase systems,cell separation

ÇOK FAZLI SİSTEMLERDE HÜCRE AYRIŞTIRILMASI

Abstract

Bu çalışma, çok fazlı polimer sistemlerinde, nanoparçacık ihtiva eden insan servikal kanser HeLa hücrelerinin, hücreler arasındaki yoğunluk farkına dayanılarak ayrıştırılması üzerinedir. Sulu çok fazlı sistemler, suda çözünen polimer ve/veya sürfaktanların çeşitli kombinasyonlarının karıştırılmasını takiben faz ayrışmasıyla oluşur. Sistemin fazları yoğunluklarına göre üst üste dizilirler ve birbirine komşu her iki fazın arasında yoğunluk-adımı olarak hareket edebilen bir interfaz bulunur. Bu interfaz, nesneleri yakalayabilecek bir bariyer görevi görür ve faz ayrışmasıyla oluştuğu için kararlıdır. Bu sistemler hazırlandıktan ve ayrıştırma için eniyilendikten sonra, hücrelerin sisteme eklenmesi ve sentrifügasyon yardımı ile ayrıştırma sağlanır. Bu çalışmada, altın nanoparçacıklarıyla kontrollü şekilde etkileşen HeLa hücrelerinin, yoğunluklarındaki değişime istinaden, sulu çok fazlı sistemlerde ayrıştırılmaları gösterilmiştir.

Keywords

• Çok fazlı sistemler,hücre ayrıştırması

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