Cell Separation with Aqueous Multiphase Systems

Year 2017, Volume: 22 Issue: 1, 65 - 80, 10.04.2017

Özge Akbulut

https://doi.org/10.17482/uumfd.305203

Abstract

This study investigates the use of multiphase
systems for density-based separation of cervical cancer HeLa cells that contain
nanoparticles. Aqueous multiphase systems (MuPSs) are prepared by mixing the
various combinations of water-soluble polymers and/or surfactants followed by
phase separation. The phases of the system are sorted according to their
density and in between neighboring phases there exists an interface that can
act as a step-in-density. This interface is also thermodynamically stable since
it is formed through phase separation. Subsequent to preparation and
optimization of these systems, cells are added to the systems and separation of
cells is achieved through centrifugation. In this work, the separation of HeLa
cells that interacted with gold nanoparticles, hence experienced a change in
their density, was demonstrated.

Keywords

Multiphase systems, cell separation

ÇOK FAZLI SİSTEMLERDE HÜCRE AYRIŞTIRILMASI

Abstract

Bu çalışma, çok fazlı polimer sistemlerinde,
nanoparçacık ihtiva eden insan servikal kanser HeLa hücrelerinin, hücreler arasındaki
yoğunluk farkına dayanılarak ayrıştırılması üzerinedir. Sulu çok fazlı
sistemler, suda çözünen polimer ve/veya sürfaktanların çeşitli
kombinasyonlarının karıştırılmasını takiben faz ayrışmasıyla oluşur. Sistemin
fazları yoğunluklarına göre üst üste dizilirler ve birbirine komşu her iki
fazın arasında yoğunluk-adımı olarak hareket edebilen bir interfaz bulunur. Bu
interfaz, nesneleri yakalayabilecek bir bariyer görevi görür ve faz
ayrışmasıyla oluştuğu için kararlıdır. Bu sistemler hazırlandıktan ve
ayrıştırma için eniyilendikten sonra, hücrelerin sisteme eklenmesi ve
sentrifügasyon yardımı ile ayrıştırma sağlanır. Bu çalışmada, altın
nanoparçacıklarıyla kontrollü şekilde etkileşen HeLa hücrelerinin,
yoğunluklarındaki değişime istinaden, sulu çok fazlı sistemlerde
ayrıştırılmaları gösterilmiştir.

Keywords

Çok fazlı sistemler, hücre ayrıştırması

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