Farklı Remineralize Edici Maddeler İçeren Ağartma Ajanlarının Sığır Diş Mine Yüzey Özellikleri Üzerine Etkilerinin Değerlendirilmesi
Year 2023,
Volume: 4 Issue: 1, 9 - 21, 07.08.2023
Hasan Hashash
Hülya Erten Can
,
Meryem Polat Gönüllü
,
Ahmet Güral
,
Hanife Altınışık
Abstract
Bu çalışmanın amacı farklı remineralize
edici maddeler içeren ağartma ajanlarının sığır
dişi minesinin mineral içeriği üzerine
etkilerinin SEM-EDX yöntemi ile
değerlendirilmesidir. Bu çalışma 32 adet sığır
dişinden elde edilen mine örnekleri üzerinde
gerçekleştirildi. Örnekler rastgele 4 gruba
ayrıldı. Grup1: %35’lik hidrojen peroksit (HP),
Grup2: %35’lik hidrojen peroksit+kalsiyum
klorür (CaCl2), Grup3: %35’lik hidrojen
peroksit+amorf kalsiyum fosfat (ACP), Grup4:
%35’lik hidrojen peroksit+kalsiyum (Ca) içeren
ağartma ajanı uygulandı. Her gruptaki mine
örnekleri iki eş parçaya ayrıldı. Bir parçasına
beyazlatma öncesi, diğer parçasına beyazlatma
sonrası SEM-EDX analizi yapıldı. Kalsiyum,
fosfat ve kalsiyum/fosfat değerleri kaydedildi.
Değişkenlerin normal dağılıp dağılmadığı
Kolmogorov-Smirnow ve Shapiro-Wilk uyum
testiyle araştırılmış ve verilerin normal
dağıldıkları görülmüştür. Gruplar arası
karşılaştırmalarda Paired Samples t-testi
kullanılmıştır. SEM-EDX analizinin sonuçlarına
göre hidrojen peroksit içeren ağartma ajanı ile
içerisine remineralize edici maddeler ilave
edilen hidrojen peroksit ağartma ajanı arasında
kalsiyum, fosfat ve kalsiyum/fosfat oranı
açısından anlamlı bir farklılık bulundu
(p<0,05). Ağartma ajanları içerisine
remineralize edici maddeler eklenmesi ağartma
sırasında oluşabilecek diş mine yüzey
demineralizasyonunu engellediği sonucuna
varıldı.
References
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L., Pucci, C. and Torres C. Effect of
hydrogen peroxide concentration on enamel
color and microhardness. Oper Dent
2014;40(1):96-101.
- 2. Cervantes, A., Bolanho, A., Valera, M.C.
and Araújo, M.A. Microhardness study of
bovine enamel to bleaching treatment
activated for different sources of light. Braz
Dent Sci. 2006;9:78-86.
- 3. Giannini, M., Silva, A.P., Cavalli, V. and
Paes Leme, A.F. Effect of carbamide
peroxide-based bleaching agents containing
fluoride or calcium on tensile strength of
human enamel. J Appl Oral Sci 2006;14:82-
7.
- 4. Tam, L. Vital tooth bleaching: Review and
current status. JCDA. 1992;58:654-659.
- 5. Attin, T., Schmidlin, P.R., Wegehaupt, F.
and Wiegand, A. Influence of study design
on the impact of bleaching agents on dental enamel microhardness: A review. Dent
Mater. 2009;25(2):143-157.
- 6. Soldani, P., Amaral, C.M., Rodrigues, J.A.
Microhardness evaluation of in situ vital
bleaching and thickening agents on human
dental enamel. Int J Periodontics
Restorative Dent. 2010;30:203-11.
- 7. Akal, N., Over, H., Olmez, A., and Bodur,
H. Effects of carbamide peroxide
containing bleaching agents on the
morphology and subsurface hardness of
enamel. J Clin Pediatr
Dent. 2001;25(4):293-296.
- 8. Lewinstein, .I, Fuhrer, N., Churaru, N. and
Cardash, H. Effect of different peroxide
bleaching regimens and subsequent
fluoridation on the hardness of human
enamel and dentin. J Prosthet Dent.
2004;92:337-42.
- 9. Pinto, C.F., Paes Leme, A.F., Cavalli, V.
and Giannini, M. Effect of 10% carbamide
peroxide bleaching on sound and artifi cial
enamel carious lesions. Braz Dent J.
2009;20:48-53.
- 10. Lia Mondelli, R. F., Garrido Gabriel, T. R.
C., Piola Rizzante, F. A., Magalhães, A. C.,
Soares Bombonatti, J. F., and Ishikiriama,
S. K. Do different bleaching protocols
affect the enamel microhardness? Eur J
Dent. 2015;1:25–30.
- 11. Thylstrup, A., Bruun, C. and Holmen, L. In
vivo caries models--mechanisms for caries
initiation and arrestment. Adv Dent Res.
1994;8(2):144-57.
- 12. White, D.J. The application of in vitro
models to research on demineralization and
remineralization of the teeth Adv Dent Res.
1995;9(3):175-193.
- 13. Rodrigues, E., Delbem, A. C., Pedrini, D.
and Cavassan, L. Enamel remineralization
by fluo-ride-releasing materials: proposal of
a pH-cycling model. Braz Dent J.
2010;21(5):446-51.
- 14. Tang, G., Yip, H.K., Cutressc, T.V. and
Samaranayakea, L.P. Artificial mouth
model systems and their contribution to
caries research. J Dent. 2003;31:161–171.
- 15. Vidhya, S., Srinivasulu, S., Sujatha, M. and
Mahalaxmi, S. Effect of grape seed extract
on the bond strength of bleached enamel.
Oper Dent. 2011;4:433-438.
- 16. Khoroushi, M. and Aghelinejad, S. Effect of
postbleaching application of anantioxidant
on enamel bond strength of three different
adhesives. Med Oral Patol Oral Cir Bucal.
2011;7:990-996.
- 17. Scougall-Vilchis, R.J., Gonzalez-Lopez,
B.S., Contreras-Bulnes, R., Rodriguez-
Vilchis, L.E., Garcia-Nino de Rivera,
M.W.and Kubodera-Ito, T. Influence of
four systems for dental bleaching on the
bond strength of orthodontic brackets.
Angle Orthod. 2011;4:700-706.
- 18. Titley, K. et al. The effect of concentrated
hydrogen peroxide on the surface
morphology of human tooth enamel. J
Endod. 1988;14(2):69-74.
- 19. Cakir, F.Y., Korkmaz, Y., Firat, E., Oztas,
S.S., and Gurgan, S. Chemical analysis of
enamel and dentin following the application
of three different athome bleaching
systems. Oper Dent. 2011;5:529-536.
- 20. Borges, A.B., Torres, C.R., de Souza, P.A.,
Caneppele, T.M., Santos, L.F. and
Magalhães, A.C. Bleaching gels containing
calcium and fluoride: effect on enamel
erosion susceptibility. Int J Dent.
2012;347848.
- 21. Hegde, M. N., Moany, A. Remineralization
of enamel subsurface lesions with casein
phosphopeptide-amorphous calcium
phosphate: A quantitative energy dispersive
X-ray analysis using scanning electron
microscopy: An in vitro study. J Conserv
Dent. 2012;1:61–67.
- 22. Sda, S. Et Al. “the effect of fluoride
therapies on the morphology of bleached
human dental enamel ferreira. Microsc Res
Tech. 2011;74(6):512-516.
- 23. Hegde, M.N., Shetty, S. and Pardal, D.
Remineralization of enamel sub-surface
lesion using casein phosphopeptideamorphous
calcium phosphate. J Conserv
Dent. 2007;10:19–25.
- 24. Bakry, A.S., Marghalani, H.Y., Amin, O.A.
and Tagami, J. The effect of a bioglass paste
on enamel exposed to erosive challenge. J
Dent. 2014;42(11):1458-1463.
- 25. Alexandria, A.K., Nassur, C., Nóbrega,
C.B.C., Valença, A.M.G., Rosalen, P.L. and
Maia, L.C. In situ effect of titanium
tetrafluoride varnish on enamel
demineralization. Braz Oral Res.
2017;6(31):e86.
- 26. Danesh-Sani, S.A., Esmaili, M. Effect of
10% sodiuascorbate hydrogel and delayed
bonding on shear bond strenght of
composite resin anda resinmodified glass
ionomer to bleached enamel. J Conserv
Dent. 2011;3:241-6
- 27. Esmaili, M Danesh-Sani, S.A.,.M ascorbate
hydrogel and delayed bonding on shear
bond strength of composite resin and
resinmodified glass ionomer to bleached
enamel. J Conserv Dent. 2011;3:241-246.
- 28. Rass, M.A. Long-term prognosis
ofinternational endodontics and biternal
bleaching of tetracycline-stained teeth.
Compendum, 1998;10:1034-1050.
- 29. Mangal, T. Vital ve devital yapılmış diş
yüzeylerine uygulanan farklı yüzey
tedavilerinin tekrar renklenmeye ve çürük
yapıcı mikrooraganizmaların tutunmasına
etkileri. Doktora Tezi, Gazi Üniversitesi
Sağlık Bilimleri Enstitüsü, Ankara,
2014:10-22.
- 30. Walstra, P., Geurts, T. J., Noomen, A.,
Jellema, A., Boekel, van, MAJS. Dairy
technology: principles of milk properties
and processes. New York: Marcel
Dekker,Inc. 1999.
- 31. Kumar, V.L., Itthagarun, A. and King, N.M.
The effect of casein phosphopeptideamorphous
calcium phosphate on
remineralization of artificial caries-like
lesions: an in vitro study. Aust Dent J.
2008;1:34-40.
- 32. Sasaki, R.T., Catelan, A., Bertoldo Edos, S.,
Venâncio, P.C., Groppo, F.C., Ambrosano,
G.M., Marchi, G.M., Lima, D.A. and
Aguiar, F.H. Effect of 7.5% hydrogen
peroxide containing remineralizing agents
on hardness, color change, roughness and
micromorphology of human enamel. J Am
Dent Assoc. 2015;5:261-7.
- 33. Borges, A. B., Samezima, L. Y., Fonseca,
L. P., Yui, K. C. K., Borges, A. L. S., &
Torres, C. R. G. Influence of potentially
remineralizing agents on bleached enamel
microhardness. Oper Dent.
2009;34(5):593-597.
- 34. Al-Salehi, S. K., Wood, D. J. And Hatton,
P. V. The effect of 24 hour non-stop
hydrogen peroxide concentration on bovine
enamel and dentine mineral content and
microhardness. J Dent. 2007;35, (11):845–
850.
- 35. Tezel, H., Ertas O.S., Ozata F., Dalgar H.,
and Korkut, Z.O. Effect of bleaching agents
on calcium loss from the enamel surface.
Quintessence Int. 2007;38 (4):339–347.
- 36. Zhang, Y. P., Din C. S., Miller S., Nathoo
S.A., and A. Gaffar. Intra-oral
remineralization of enamel with a
MFP/DCPD and MFP/silica dentifrice
using surface microhardness. J Clin
Dent.1995;6(2):148–153.
- 37. Attin, T., Kocabiyik M., Buchalla W.,
Hannig C., and Becker K. Susceptibility of
enamel surfaces to demineralization after
application of fluoridated carbamide
peroxide gels. Caries Res. 2003;37(2):93–
99.
- 38. Hughes, J. A., West, N. X. Parker, D. M.
van den Braak, M. H. and Addy, M. Effects
of pH and concentration of citric, malic and
lactic acids on enamel, in vitro. J Dent.
2000;28(2):147–152.
- 39. De Oliveira, R., Paes Leme, A. F., and
Giannini, M. Effect of a carbamide peroxide
bleaching gel containing calcium or
fluoride on human enamel surface
microhardness. Braz. Dent. J.
2004;16(2):103–106.
Assessment Of The Effects Of Bleachıng Agents Contaınıng Dıfferent Remıneralısıng Materıals On Bovıne Teeth Enamel Surface Propertıes
Year 2023,
Volume: 4 Issue: 1, 9 - 21, 07.08.2023
Hasan Hashash
Hülya Erten Can
,
Meryem Polat Gönüllü
,
Ahmet Güral
,
Hanife Altınışık
Abstract
The aim of this study was to
evaluate the effects of bleaching agents
containing different remineralizing agents on
mineral content of bovine enamel by SEM-EDX
method. This study was conducted on enamel
samples obtained from 32 bovine teeth. The
samples were randomly divided into 4 groups.
Group1: 35% hydrogen peroxide containing
bleaching agent, Group2: 35% hydrogen
peroxide + calcium chloride, Group3: 35%
hydrogen peroxide + amorphous calcium
phosphate, Group4: 35% hydrogen peroxide +
calcium containing bleaching agent was
applied. Enamel samples in each group were
divided into two identical parts. SEM-EDX
analysis was performed before bleaching on
one part and after bleaching on the other part.
Calcium, phosphate and calcium/phosphate
values were recorded. Whether variables are
normally distributed or not has been
investigated with the Kolmogorov-Smirnov
and Shapiro-Wilk compliance tests and the data
were found to be normally distributed. Paired
Samples t-test was used in comparisons
between groups. According to the results of
SEM-EDX analysis, a significant difference was
found between the hydrogen peroxide
bleaching agent and the hydrogen peroxide
bleaching agent, in which remineralizing
agents were added, in terms of calcium,
phosphate and calcium/phosphate ratio (p
<0.05). It was concluded that the addition of
remineralizing agents to bleaching agents
prevents the surface enamel demineralization
during bleaching.
References
- 1. Borges, A.B., Zanatta, R., Barros, A., Silva,
L., Pucci, C. and Torres C. Effect of
hydrogen peroxide concentration on enamel
color and microhardness. Oper Dent
2014;40(1):96-101.
- 2. Cervantes, A., Bolanho, A., Valera, M.C.
and Araújo, M.A. Microhardness study of
bovine enamel to bleaching treatment
activated for different sources of light. Braz
Dent Sci. 2006;9:78-86.
- 3. Giannini, M., Silva, A.P., Cavalli, V. and
Paes Leme, A.F. Effect of carbamide
peroxide-based bleaching agents containing
fluoride or calcium on tensile strength of
human enamel. J Appl Oral Sci 2006;14:82-
7.
- 4. Tam, L. Vital tooth bleaching: Review and
current status. JCDA. 1992;58:654-659.
- 5. Attin, T., Schmidlin, P.R., Wegehaupt, F.
and Wiegand, A. Influence of study design
on the impact of bleaching agents on dental enamel microhardness: A review. Dent
Mater. 2009;25(2):143-157.
- 6. Soldani, P., Amaral, C.M., Rodrigues, J.A.
Microhardness evaluation of in situ vital
bleaching and thickening agents on human
dental enamel. Int J Periodontics
Restorative Dent. 2010;30:203-11.
- 7. Akal, N., Over, H., Olmez, A., and Bodur,
H. Effects of carbamide peroxide
containing bleaching agents on the
morphology and subsurface hardness of
enamel. J Clin Pediatr
Dent. 2001;25(4):293-296.
- 8. Lewinstein, .I, Fuhrer, N., Churaru, N. and
Cardash, H. Effect of different peroxide
bleaching regimens and subsequent
fluoridation on the hardness of human
enamel and dentin. J Prosthet Dent.
2004;92:337-42.
- 9. Pinto, C.F., Paes Leme, A.F., Cavalli, V.
and Giannini, M. Effect of 10% carbamide
peroxide bleaching on sound and artifi cial
enamel carious lesions. Braz Dent J.
2009;20:48-53.
- 10. Lia Mondelli, R. F., Garrido Gabriel, T. R.
C., Piola Rizzante, F. A., Magalhães, A. C.,
Soares Bombonatti, J. F., and Ishikiriama,
S. K. Do different bleaching protocols
affect the enamel microhardness? Eur J
Dent. 2015;1:25–30.
- 11. Thylstrup, A., Bruun, C. and Holmen, L. In
vivo caries models--mechanisms for caries
initiation and arrestment. Adv Dent Res.
1994;8(2):144-57.
- 12. White, D.J. The application of in vitro
models to research on demineralization and
remineralization of the teeth Adv Dent Res.
1995;9(3):175-193.
- 13. Rodrigues, E., Delbem, A. C., Pedrini, D.
and Cavassan, L. Enamel remineralization
by fluo-ride-releasing materials: proposal of
a pH-cycling model. Braz Dent J.
2010;21(5):446-51.
- 14. Tang, G., Yip, H.K., Cutressc, T.V. and
Samaranayakea, L.P. Artificial mouth
model systems and their contribution to
caries research. J Dent. 2003;31:161–171.
- 15. Vidhya, S., Srinivasulu, S., Sujatha, M. and
Mahalaxmi, S. Effect of grape seed extract
on the bond strength of bleached enamel.
Oper Dent. 2011;4:433-438.
- 16. Khoroushi, M. and Aghelinejad, S. Effect of
postbleaching application of anantioxidant
on enamel bond strength of three different
adhesives. Med Oral Patol Oral Cir Bucal.
2011;7:990-996.
- 17. Scougall-Vilchis, R.J., Gonzalez-Lopez,
B.S., Contreras-Bulnes, R., Rodriguez-
Vilchis, L.E., Garcia-Nino de Rivera,
M.W.and Kubodera-Ito, T. Influence of
four systems for dental bleaching on the
bond strength of orthodontic brackets.
Angle Orthod. 2011;4:700-706.
- 18. Titley, K. et al. The effect of concentrated
hydrogen peroxide on the surface
morphology of human tooth enamel. J
Endod. 1988;14(2):69-74.
- 19. Cakir, F.Y., Korkmaz, Y., Firat, E., Oztas,
S.S., and Gurgan, S. Chemical analysis of
enamel and dentin following the application
of three different athome bleaching
systems. Oper Dent. 2011;5:529-536.
- 20. Borges, A.B., Torres, C.R., de Souza, P.A.,
Caneppele, T.M., Santos, L.F. and
Magalhães, A.C. Bleaching gels containing
calcium and fluoride: effect on enamel
erosion susceptibility. Int J Dent.
2012;347848.
- 21. Hegde, M. N., Moany, A. Remineralization
of enamel subsurface lesions with casein
phosphopeptide-amorphous calcium
phosphate: A quantitative energy dispersive
X-ray analysis using scanning electron
microscopy: An in vitro study. J Conserv
Dent. 2012;1:61–67.
- 22. Sda, S. Et Al. “the effect of fluoride
therapies on the morphology of bleached
human dental enamel ferreira. Microsc Res
Tech. 2011;74(6):512-516.
- 23. Hegde, M.N., Shetty, S. and Pardal, D.
Remineralization of enamel sub-surface
lesion using casein phosphopeptideamorphous
calcium phosphate. J Conserv
Dent. 2007;10:19–25.
- 24. Bakry, A.S., Marghalani, H.Y., Amin, O.A.
and Tagami, J. The effect of a bioglass paste
on enamel exposed to erosive challenge. J
Dent. 2014;42(11):1458-1463.
- 25. Alexandria, A.K., Nassur, C., Nóbrega,
C.B.C., Valença, A.M.G., Rosalen, P.L. and
Maia, L.C. In situ effect of titanium
tetrafluoride varnish on enamel
demineralization. Braz Oral Res.
2017;6(31):e86.
- 26. Danesh-Sani, S.A., Esmaili, M. Effect of
10% sodiuascorbate hydrogel and delayed
bonding on shear bond strenght of
composite resin anda resinmodified glass
ionomer to bleached enamel. J Conserv
Dent. 2011;3:241-6
- 27. Esmaili, M Danesh-Sani, S.A.,.M ascorbate
hydrogel and delayed bonding on shear
bond strength of composite resin and
resinmodified glass ionomer to bleached
enamel. J Conserv Dent. 2011;3:241-246.
- 28. Rass, M.A. Long-term prognosis
ofinternational endodontics and biternal
bleaching of tetracycline-stained teeth.
Compendum, 1998;10:1034-1050.
- 29. Mangal, T. Vital ve devital yapılmış diş
yüzeylerine uygulanan farklı yüzey
tedavilerinin tekrar renklenmeye ve çürük
yapıcı mikrooraganizmaların tutunmasına
etkileri. Doktora Tezi, Gazi Üniversitesi
Sağlık Bilimleri Enstitüsü, Ankara,
2014:10-22.
- 30. Walstra, P., Geurts, T. J., Noomen, A.,
Jellema, A., Boekel, van, MAJS. Dairy
technology: principles of milk properties
and processes. New York: Marcel
Dekker,Inc. 1999.
- 31. Kumar, V.L., Itthagarun, A. and King, N.M.
The effect of casein phosphopeptideamorphous
calcium phosphate on
remineralization of artificial caries-like
lesions: an in vitro study. Aust Dent J.
2008;1:34-40.
- 32. Sasaki, R.T., Catelan, A., Bertoldo Edos, S.,
Venâncio, P.C., Groppo, F.C., Ambrosano,
G.M., Marchi, G.M., Lima, D.A. and
Aguiar, F.H. Effect of 7.5% hydrogen
peroxide containing remineralizing agents
on hardness, color change, roughness and
micromorphology of human enamel. J Am
Dent Assoc. 2015;5:261-7.
- 33. Borges, A. B., Samezima, L. Y., Fonseca,
L. P., Yui, K. C. K., Borges, A. L. S., &
Torres, C. R. G. Influence of potentially
remineralizing agents on bleached enamel
microhardness. Oper Dent.
2009;34(5):593-597.
- 34. Al-Salehi, S. K., Wood, D. J. And Hatton,
P. V. The effect of 24 hour non-stop
hydrogen peroxide concentration on bovine
enamel and dentine mineral content and
microhardness. J Dent. 2007;35, (11):845–
850.
- 35. Tezel, H., Ertas O.S., Ozata F., Dalgar H.,
and Korkut, Z.O. Effect of bleaching agents
on calcium loss from the enamel surface.
Quintessence Int. 2007;38 (4):339–347.
- 36. Zhang, Y. P., Din C. S., Miller S., Nathoo
S.A., and A. Gaffar. Intra-oral
remineralization of enamel with a
MFP/DCPD and MFP/silica dentifrice
using surface microhardness. J Clin
Dent.1995;6(2):148–153.
- 37. Attin, T., Kocabiyik M., Buchalla W.,
Hannig C., and Becker K. Susceptibility of
enamel surfaces to demineralization after
application of fluoridated carbamide
peroxide gels. Caries Res. 2003;37(2):93–
99.
- 38. Hughes, J. A., West, N. X. Parker, D. M.
van den Braak, M. H. and Addy, M. Effects
of pH and concentration of citric, malic and
lactic acids on enamel, in vitro. J Dent.
2000;28(2):147–152.
- 39. De Oliveira, R., Paes Leme, A. F., and
Giannini, M. Effect of a carbamide peroxide
bleaching gel containing calcium or
fluoride on human enamel surface
microhardness. Braz. Dent. J.
2004;16(2):103–106.