A case of papillary and infiltrative urothelial carcinoma of the urinary bladder in a terrier dog

Year 2023, , 66 - 72, 30.04.2023

Gökhan Akçakavak , Zeynep Çelik , Elgin Orçum Uzunlu , Muhammed Öner , Mehmet Tuzcu , Mustafa Arıcan

https://doi.org/10.31797/vetbio.1237692

Abstract

Urothelial carcinomas are malignant tumours originating from the epithelial layer of the urinary bladder. In this instance, a case of papillary and infiltrative urothelial carcinoma in the urinary bladder of a 2-year-old terrier dog was defined clinically, histopathologically and immunohistochemically. The material of the presented case consisted of urinary bladder tissue samples that were surgically extirpated from a two-year-old terrier-breed female dog that applied to the Department of Surgery of the Faculty of Veterinary Medicine, Selcuk University with the complaint of hematuria. The tumour brought to the pathology laboratory was 11*10*12 cm in size and had finger-shaped extensions. Its outer surface was rough and hemorrhagic. Tissues were fixed in 10% buffered formalin and paraffin blocks were obtained by going through the necessary routine follow-up procedures. Afterward, sections were taken and subjected to Hematoxylin-Eosin, Masson's Trichrome, and immunohistochemical staining. As a result of the pathological and immunohistochemical examinations of the tumoral tissue samples taken from the urinary bladder, the diagnosis of papillary and infiltrative urothelial carcinoma was reached, and the case was discussed with the information provided by the literature. In addition, immunohistochemically, intense Proliferating cell nuclear antigen (PCNA) and Vascular endothelial growth factor (VEGF) staining has been associated with malignancy.

Keywords

Urinary bladder, urothelial carcinoma, dog, histopathology

References

• Ahmed, I., and Sozmen, M. (2020). Proliferating cell nuclear antigen expression in canine and feline spontaneous and injection-site fibrosarcomas. Pakistan Veterinary Journal, 40(4), 531-533. doi: 10.29261/pakvetj/2020.019
• Aydogan, A., Ozmen, O., Haligur, M., Sipahi, C., Ileri, D., & Haligur, A. (2018). Immunohistochemical evaluation of bcl-2, ER-alpha, caspase-3,-8,-9, PCNA and Ki- 67 expressions in canine mammary carcinomas. Biotechnic & Histochemistry, 93(4), 286-292. 10.1080/10520295.2018.1434680
• Campos, A. G., Campos, J. A. D. B., Sanches, D. S., Dagli, M. L. Z., & Matera, J. M. (2012). Immunohistochemical evaluation of vascular endothelial growth factor (VEGF) in splenic hemangiomas and hemangiosarcomas in dogs. 2(4), doi: 10.4236/ojvm.2012.24030
• Erer, H., & Kıran, M. (2009). Veteriner Onkoloji (7rd ed.). Nobel Tıp Kitabevleri, 2021.
• Fulkerson, C. M., & Knapp, D. W. (2015). Management of transitional cell carcinoma of the urinary bladder in dogs: a review. The veterinary journal, 205(2), 217-225. 10.1016/j.tvjl.2015.01.017.
• Güzel, Ö. (2006). Kedi ve köpeklerde üriner sistem tümörlerinin tani ve sağaltimi. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 32(2), 11-21.
• Haigh, J. J., Gerber, H.-P., Ferrara, N., & Wagner, E. F. (2000). Conditional inactivation of VEGF-A in areas of collagen2a1 expression results in embryonic lethality in the heterozygous state. development, 127(7), 1445-1453. doi: 10.1242/dev.127.7.1445
• Kent, M. S., Burton, J. H., Dank, G., Bannasch, D. L., & Rebhun, R. B. (2018). Association of cancer-related mortality, age and gonadectomy in golden retriever dogs at a veterinary academic center (1989-2016). PloS one, 13(2), e0192578. doi: 10.1371/journal.pone.0192578
• Knapp, D. W., Ramos-Vara, J. A., Moore, G. E., Dhawan, D., Bonney, P. L., & Young, K. E. (2014). Urinary bladder cancer in dogs, a naturally occurring model for cancer biology and drug development. ILAR journal, 55(1), 100-118. doi:10.1093/ilar/ilu018
• Kok, M. K., Chambers, J. K., Tsuboi, M., Nishimura, R., Tsujimoto, H., Uchida, K., & Nakayama, H. (2019). Retrospective study of canine cutaneous tumors in Japan, 2008–2017. Journal of veterinary medical science, 81(8), 1133-1143. doi: 10.1292/jvms.19-0248.
• Krishna, K. R., Latha, M. J., Lakshman, M., Rani, M. U., Kumar, Y. R., & Swathi, B. (2022). Expression pattern of PCNA and Ki-67 biomarkers in canine mammary tumours. The Pharma Innovation Journal, 11(5), 233-237.
• Maga, G., & Hubscher, U. (2003). Proliferating cell nuclear antigen (PCNA): a dancer with many partners. Journal of cell science, 116(15), 3051-3060. doi: 10.1242/jcs.00653.
• Mantovani, A., Schioppa, T., Porta, C., Allavena, P., & Sica, A. (2006). Role of tumor-associated macrophages in tumor progression and invasion. Cancer and Metastasis Reviews, 25(3), 315-322. doi: 10.1007/s10555-006-9001-7.
• Martano, M., Restucci, B., Ceccarelli, D. M., Lo Muzio, L., & Maiolino, P. (2016). Immunohistochemical expression of vascular endothelial growth factor in canine oral squamous cell carcinomas. Oncology letters, 11(1), 399-404. doi: 10.3892/ol.2015.3847
• Maxie, M. G. (2015). Jubb, Kennedy & Palmer's pathology of domestic animals (Vol. 2). Elsevier health sciences.
• Meuten, D. J. (2017). Tumors in domestic animals (5rd ed.), John Wiley & Sons.
• Moulton, J. (1990). Tumors in domestic animals (3rd ed.), University of California Press, Berkeley.
• Norris, A. M., Laing, E. J., Valli, V. E., Withrow, S. J., Macy, D. W., Ogilvie, G. K., Tomlinson, J., McCaw, D., Pidgeon, G., & Jacobs, R. M. (1992). Canine bladder and urethral tumors: a retrospective study of 115 cases (1980–1985). Journal of Veterinary Internal Medicine, 6(3), 145-153. doi: 10.1111/j.1939-1676.1992.tb00330.x.
• Ozdemir, O., Akcakavak, G., & Tuzcu, M. (2022). Effect of Tarantula cubensis alcoholic extract and Nerium oleander distillate on cell proliferation markers in colon carcinogenesis. Revista Científica de Veterinaria, 32, 1-8, doi: 10.52973/rcfcv-e32150
• Pradhan, S., Kalia, I., Roy, S. S., Singh, O. P., Adak, T., Singh, A. P., & Dhar, S. K. (2019). Molecular characterization and expression profile of an alternate proliferating cell nuclear antigen homolog PbPCNA2 in Plasmodium berghei. IUBMB life, 71(9), 1293-1301. doi: 10.1002/iub.2036.
• Restucci, B., Maiolino, P., Paciello, O., Martano, M., De Vico, G., & Papparella, S. (2003). Evaluation of angiogenesis in canine seminomas by quantitative immunohistochemistry. Journal of comparative pathology, 128(4), 252-259. doi: 10.1053/jcpa.2002.0630.
• Sia, D., Alsinet, C., Newell, P., & Villanueva, A. (2014). VEGF signaling in cancer treatment. Current pharmaceutical design, 20(17), 2834-2842. doi: 10.2174/13816128113199990590.
• Simons, M., Gordon, E., & Claesson-Welsh, L. (2016). Mechanisms and regulation of endothelial VEGF receptor signalling. Nature reviews Molecular cell biology, 17(10), 611-625.
• Strafuss, A., & Dean, M. (1975). Neoplasms of the canine urinary bladder. Journal of the American Veterinary Medical Association, 166(12), 1161-1163.
• Tehseen, M., Raducanu, V.-S., Rashid, F., Shirbini, A., Takahashi, M., & Hamdan, S. M. (2019). Proliferating cell nuclear antigen-agarose column: A tag-free and tag-dependent tool for protein purification affinity chromatography. Journal of Chromatography A, 1602, 341-349. doi: 10.1016/j.chroma.2019.06.008.
• Tuzcu, M., Tuzcu, N., Akcakavak, G., & Celik, Z. (2022). Diagnosis of Sarcina ventriculi-derived haemorrhagic abomasitis in lambs by histopathology and real-time PCR. Acta Veterinaria Brno, 91(3), 227-233. doi: 10.2754/avb202291030227
• Ye, X., Ling, B., Xu, H., Li, G., Zhao, X., Xu, J., Liu, J., & Liu, L. (2020). Clinical significance of high expression of proliferating cell nuclear antigen in non-small cell lung cancer. Medicine, 99(16).
• Yönez, M. K. (2016). Üriner Sistem Tümörleri. Turkiye Klinikleri Veterinary Sciences-Surgery-Special Topics, 2(2), 34-39.