Research Article

Effect of caffeic acid on freezing of epididymal sperm in domestic cat

Number: 2 May 7, 2026

Effect of caffeic acid on freezing of epididymal sperm in domestic cat

Abstract

The aim of this study was to investigate the effect of caffeic acid on the freezing of epididymal cat sperm. After castration, cauda epididymis were obtained from 10 cat testes. Epidididymal spermatozoa were analysed for motility, viability, and concentration were evaluated and mixed. The mixed sperm was diluated with containing different amounts of caffeic acid (0.003 mg/mL (A1), 0.006 mg/mL (A2) and 0.010 mg/mL (A3)) and without caffeic acid (control). The groups were cryopreservation in liquid nitrogen vapour and the samples were thawed at 37°C for 30 seconds. After thawing, motility, viability, abnormal spermatozoa ratio, and HOS test were evaluated. As a result of the evaluation, although the caffeic acid groups were numerically higher than the control, they were not statistically different (P>0.05). It is concluded that caffeic acid supports spermatological parameters but future applications are needed to establish a standard freezing protocol.

Keywords

References

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Details

Primary Language

English

Subjects

Reproduction and Artificial Insemination

Journal Section

Research Article

Publication Date

May 7, 2026

Submission Date

July 24, 2025

Acceptance Date

May 4, 2026

Published in Issue

Year 2026 Number: 2

APA
Gökdemir, T., Tanrıverdi, E., Kılıç, M. C., Şahin, O., Yılmaz, A., Yıldız, S., Ermutlu, C. Ş., Önder, N. T., Aydın, U., & Öztürkler, Y. (2026). Effect of caffeic acid on freezing of epididymal sperm in domestic cat. Journal of Advances in VetBio Science and Techniques, 2, 105-109. https://doi.org/10.31797/vetbio.1749776
AMA
1.Gökdemir T, Tanrıverdi E, Kılıç MC, et al. Effect of caffeic acid on freezing of epididymal sperm in domestic cat. J Ad VetBio Sci Tech. 2026;(2):105-109. doi:10.31797/vetbio.1749776
Chicago
Gökdemir, Taygun, Ersin Tanrıverdi, Muhammet Can Kılıç, et al. 2026. “Effect of Caffeic Acid on Freezing of Epididymal Sperm in Domestic Cat”. Journal of Advances in VetBio Science and Techniques, nos. 2: 105-9. https://doi.org/10.31797/vetbio.1749776.
EndNote
Gökdemir T, Tanrıverdi E, Kılıç MC, Şahin O, Yılmaz A, Yıldız S, Ermutlu CŞ, Önder NT, Aydın U, Öztürkler Y (May 1, 2026) Effect of caffeic acid on freezing of epididymal sperm in domestic cat. Journal of Advances in VetBio Science and Techniques 2 105–109.
IEEE
[1]T. Gökdemir et al., “Effect of caffeic acid on freezing of epididymal sperm in domestic cat”, J Ad VetBio Sci Tech, no. 2, pp. 105–109, May 2026, doi: 10.31797/vetbio.1749776.
ISNAD
Gökdemir, Taygun - Tanrıverdi, Ersin - Kılıç, Muhammet Can - Şahin, Oğuzhan - Yılmaz, Alican - Yıldız, Savaş - Ermutlu, Celal Şahin - Önder, Nail Tekin - Aydın, Uğur - Öztürkler, Yavuz. “Effect of Caffeic Acid on Freezing of Epididymal Sperm in Domestic Cat”. Journal of Advances in VetBio Science and Techniques. 2 (May 1, 2026): 105-109. https://doi.org/10.31797/vetbio.1749776.
JAMA
1.Gökdemir T, Tanrıverdi E, Kılıç MC, Şahin O, Yılmaz A, Yıldız S, Ermutlu CŞ, Önder NT, Aydın U, Öztürkler Y. Effect of caffeic acid on freezing of epididymal sperm in domestic cat. J Ad VetBio Sci Tech. 2026;:105–109.
MLA
Gökdemir, Taygun, et al. “Effect of Caffeic Acid on Freezing of Epididymal Sperm in Domestic Cat”. Journal of Advances in VetBio Science and Techniques, no. 2, May 2026, pp. 105-9, doi:10.31797/vetbio.1749776.
Vancouver
1.Taygun Gökdemir, Ersin Tanrıverdi, Muhammet Can Kılıç, Oğuzhan Şahin, Alican Yılmaz, Savaş Yıldız, Celal Şahin Ermutlu, Nail Tekin Önder, Uğur Aydın, Yavuz Öztürkler. Effect of caffeic acid on freezing of epididymal sperm in domestic cat. J Ad VetBio Sci Tech. 2026 May 1;(2):105-9. doi:10.31797/vetbio.1749776

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