Ketamine-Ksilazin Anestezisi Uygulanmış Bozova Tazılarında Oksidatif Stres Parametreleri

Öz

Bu çalışma ksilazin-ketamin anestezisinin Bozova tazılarında bazı iz element (Cu, Zn, Mn) ve oksidatif stres parametreleri (SOD, CAT, GPx, MDA) üzerine etkilerini değerlendirmek amacıyla yapıldı. Farklı yaş, vücut ağırlığı ve cinsiyetteki toplam sekiz tazı kullanıldı. Farklı şikâyetlerle gelen bu hayvanlara Ketamin (10mg/kg) ve ksilazin (1 mg / k g) kas içi enjekte edildi. Anestezi öncesi ve anestezi sırasında antikoagulanlı ve antikoagulansız tüplere kan örnekleri alındı. Kan örnekleri 3000 rpm’de 10 dk santrifüje edilerek aynı gün değerlendirildi. Eritrosit süperoksit dismutaz (SOD), Glutatyon Peroksidaz (GPx), malondialdehit (MDA) ve plazma Katalaz (CAT) değerleri standart metotlarla, serum bakır, çinko, manganez düzeyleri ICP-OES ile belirlendi. Bu çalışmada; SOD aktivitesinin anestezi sonrasında düşük (p<0,05) olduğu belirlenirken GPx, MDA,  CAT, Cu, Zn ve Mn seviyelerinde istatistiksel olarak önemli bir fark belirlenmedi (p>0.05). Sonuç olarak, ketamin ve ksilazin anestezisinin Bozova tazılarında serum Cu, Zn, Mn seviyeleri ve oksidatif stres yönünden güvenli ve kullanılabilir olduğu söylenebilir.

Anahtar Kelimeler

• Bozova tazısı,oxidatif stres,SOD,CAT,GPx,MDA,Cu,Zn

Oxidative Stress Parameters in Xylasine-Ketamine Adminestered Bozova Greyhounds

Abstract

The present study was conducted to evaluate the effects of xylaine-ketamine anesthezia on some micro elements and oxidative stress parameters in Bozova greyhounds. In the study eight male greyhounds, which were in different age and body weight were used as the material. The animals, which were, came in clinic with different complaints injected with the ketamine (10 mg/kg) and xylazine (1 mg/kg) intramuscularly. The findings were recorded before and during anesthesia. Blood samples were collected from the vena cephalica. Serum copper, zinc, manganese levels were determined by an ICP-OES. Superoxide dismutase (SOD), glutathione peroxidase (GPx), malondialdehyde (MDA) and catalase (CAT) were measured by validated standard methods. In this study, while SOD activity was lower (p<0,05) in the post-anesthezia; a statistically significant difference wasn't determined between the levels of Cu, Zn, Mn and, CAT, GPx, MDA (p>0.05). In conclusion, we regard to it was seen that sera copper, zinc, manganese levels and oxidative stress parameters, ketamine-xylazine anesthesiz can be used safely in Bozova greyhounds

Keywords

• Bozova Greyhounds,oxidative stress,SOD,CAT,GPx,MDA,Cu,Zn

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