Klinoptilolit kuzularda giardiazis sağaltımına yönelik alternatif ve doğal bir çözüm olabilir mi?

Year 2018, , 16 - 20, 31.08.2018

Adnan Ayan , Deniz Alıç Ural , Serdar Paşa Songül Erdoğan Hasan Erdoğan

https://doi.org/10.31797/vetbio.411022

Abstract

Amaç: Bu saha çalışması giardiasisli kuzularda oral yolla uygulanan klinoptilolitin etkinliğinin
belirlenmesi amacıyla gerçekleştirildi.
Yöntemler: Aydın ili sınırlarında yer alan özel bir işletmede ishal mevcut ve G. duodenalis ile
doğal enfekte her iki cinsiyetten ve 23-56 günlük yaştaki 14 kuzu değerlendirildi. Tanı amacıyla
ELISA prensibi ile çalışan hızlı tanısal test kitleri yapıldı. Giardiazisli kuzular her grupta n=7
olarak rastgele I. gruba [1 g/kg dozda 10 gün oral yolla klinoptilolit uygulanırken] ya da II. gruba
[ilaç uygulaması yapılmaksızın kontrol grubu] ayırıldı. Klinoptilolitin sağaltım etkinliği 0. ve 10.
günlerde rektal yolla elde edilen dışkı örneklerinin mikroskobik muayenesiyle değerlendirildi.
Bulgular: Kontrol ve sağaltım grupları arasında karşılaştırmalarda 10. günde klinoptilolit ile
sağaltılan grupta kist atılımında % 97,2’lik azalma belirgindi (p 0˂0,05).
Sonuç: Giardiazisli kuzularda oral yolla 10 gün uygulanan klinoptilolitin kist atılımını etkin
şekilde azalttığı ve antiparaziter etkinlik sağladığı görüldü.

Keywords

Kuzu, giardiazis, klinoptilolit

May clinoptilolite be an alternative and natural solution for the treatment of giardiasis in lambs?

Abstract

Objective The aim of this field study was to detect the therapeutic efficacy of oral clinoptilolite
in lambs with giardiasis.
Methods: A total of fourteen lambs naturally infected with G. duodenalis at the age of 23 to 56
days, of both sexes were enrolled into the study from a lamb farm located in Aydin province.
Diagnosis was based on rapid diagnositc test kits with ELISA principle. Lambs with giardiasis
were randomly assigned into two groups (n=7), group I [1 g/kg clinoptilolite for 10 days or group
II [were left as control without receiving any drug]. Therapeutic efficacy of clinoptilolite was
determined by microscopical examination of faecal samples collected rectally on days 0 and 10.
Results: Comparison of control and treatment intragroups revealed 97,2% reduction (p 0˂0,05)
for cyst excretion in clinoptilolite treated group.
Conclusion: In conclusion, it may be safey suggested that oral clinoptilolite administration for
10 days effectively reduct cyst excretion with antiparasitic efficacy among lambs with giardiasis.

Keywords

Lamb, giardiasis, clinoptilolite

References

• Alıç Ural, D., Aysul, N., Gultekin, M. (2016). The Efficacy of Oral Administration of Clinoptilolite Against Naturally Occuring Giardiasis in Calves. Kocatepe Vet J, 9(4), 288-293. DOI: 10.5578/kvj.28122Alıç Ural, D., Erdoğan, H., Toplu, S., Ayan, A. (2017). Oğlaklarda Giardiazis Kontrolüne Yönelik Oral Klinoptilolit Uygulaması. Kocatepe Vet J, 10(3), 158-163. DOI:10.5578/kvj.57512Aloisio, F., Filippini, G., Antenucci, P., Lepri, E., Pezzotti, G., Cacciò, SM. (2006). Severe weight loss in lambs infected with Giardia duodenalis assemblage B. Vet Parasitol, 142(1-2), 154-158. DOI:10.1016/j.vetpar.2006.06.023Ayan, A., Ural, K., Aysul, N., Gültekin, M., Erdoğan, H., Balıkçı, C., Toplu, S., Toros, G. (2016). Natural Cyst Shedding in Calves Infected with Giardia duodenalis. JAVST, 1(1), 14-19.Caccio, S.M. (2015). Giardiasis: a zoonotic infection or not? In: Sing A (ed’s), Zoonoses-ınfections affecting humans and animals. New-York (NY), Springer, 821-848.Cacció, S.M., de Giacomo, M., Pozio, E. (2002). Sequence analysis of the β giardin gene and development of a PCR-RFLP assay to genotype Giardia duodenalis cysts from human faecal samples. Int J Parasitol, 32(8), 1023-1030. DOI:10.1016/S0020-7519(02)00068-1Ey, PL., Mansouri, M., Kulda, J., Nohynkova, E., Monis, P.T., Andrews, R.H., Mayrhofer, G. (1997). Genetic analysis of Giardia from hoofed farm animals reveals artiodactyls-specific and potentially zoonotic genotypes. J Eukaryot Microbiol, 44(6), 625-635. Geurden, T., Thomas, P., Casaert, S., Vercruysse, J., Claerebout, E. (2008). Prevalence and molecular characterization of Cryptosporidium and Giardia in lambs and goat kids in Belgium. Vet Parasitol, 155(1-2), 142-145. DOI:10.1016/j.vetpar.2008.05.002Geurden, T., Vercruysse, J., Claerebout, E. (2010). Is Giardia a signifcant pathogen in production animals? Exp Parasitol, 124(1), 98-106. DOI:10.1016/j.exppara.2009.03.001
• Geurden, T., Pohleb, H., Sarrea, C., Dreesena, L., Vercruyssea, J., Claerebouta, E. (2011). The effcacy of a treatment with fenbendazole against an experimental Giardia duodenalis infection in lambs. Small Rum Res, 96(2-3), 211-215. DOI:10.1016/j.smallrumres.2010.12.004Giangaspero, A., Paoletti, B., Iorio, R., Traversa, D. (2005). Prevalence and molecular characterization of Giardia duodenalis from sheep in central Italy. J Parasitol Res, 96(1), 32-37. DOI:10.1007/s00436-005-1317-7Gultekin, M., Ural, K., Aysul, N., Ayan, A., Balikci, C., Akyildiz, G. (2016). The efficacy of chloroquine treatment of Giardia duodenalis infection in calves. Vlaams Diergeneeskd Tijdschr, 85(6): 335-41.Gultekin, M., Ural, K., Aysul, N., Ayan, A., Balikci, C., Akyildiz, G. (2017). Prevalence and molecular characterization of Giardia duodenalis in dogs in Aydin, Turkey. Int J Environ Health Res, 27(3), 161-168. DOI:10.1080/09603123.2017.1310187Gultekin, M., Ural, K., Aysul, N., Ayan, A., Balikci, C., Toplu, S., Akyildiz, G. (2017). Prevalence and molecular characterization of Giardia duodenalis in calves in Turkey. Acta Sci Vet, 45(1450), 1-6. DOI:10.22456/1679-9216.80032Henderson, D.F., Tilton, E.W. (1955). Tests with acaricides against the brown wheat mite. J Econ Entomol, 48(2), 157-161. DOI:10.1093/jee/48.2.157Karahalli C, Ural K. (2017). Single dose secnidazol treatment efficacy against naturally occurring Giardia duodenalis infection in dogs. Magy Allatorvosok Lapja, 139(10), 621-30.Karanis, P., Ey, P.L. (1998). Characterization of axenic isolates of Giardia intestinalis established from humans and animals in Germany. J Parasitol Res, 84, 442-449.Lalle, M., Pozio, E., Capelli, G., Bruschi, F., Crotti, D., Cacció, S.M. (2005). Genetic heterogeneity at the ß-giardin locus among human and animal isolates of Giardia duodenalis and identification of potentially zoonotic subgenotypes. Int J Parasitol, 35(2), 207-213. DOI: 10.1016/j.ijpara.2004.10.022Lebbad, M., Mattsson, J.G., Christensson, B., Ljungström, B., Backhans, A., Andersson, J.O., Svärd, S.G. (2010). From mouse to moose: multilocus genotyping of Giardia isolates from various animal species. Vet Parasitol, 168(3-4), 231-239. DOI:10.1016/j.vetpar.2009.11.003O’Handley, R.M., Cockwill, C., Jelinski, M., McAllister, T.A., Olson, M.E. (2000). Effects of repeat fenbendazole treatment in dairy calves with giardiosis on cyst excretion, clinical signs and production. Vet Parasitol, 89(3), 209-218. DOI:10.1016/S0304-4017(00)00200-4Olson, M.E., O’Handley, R.M., Ralston, B.J., McAllister, TA., Thompson, R.C. (2004). Update on Cryptosporidium and Giardia infections in cattle. Trends Parasitol, 20(4), 185-191. DOI:10.1016/j.pt.2004.01.015Prado, M.S., Cairncross, S., Strina, A., Barreto, M.L., Oliveira-Assis, A.M., Rego, S. (2005). Asymptomatic giardiasis and growth in young children; a longitudinal study in Salvador, Brazil. Parasitology, 131(1), 51-56. DOI:10.1017/S0031182005007353Ragbetli, C., Tanritanir, P., Yilmaz, H., Yoruk, I., Ozdemir, H. (2014). Effect on biochemical parameters in naturally infected calves with giardiasis after treatment with albendazole in Van Province, Turkey. Indian J Anim Sci, 48(1), 38-44. DOI:10.5958/j.0976-0555.48.1.008Robertson, L.J. (2009). Giardia and Cryptosporidium infections in sheep and goats: A review of the potential for transmission to humans via environmental contamination. Epidemiol Infect, 137(7), 913-921. DOI: 10.1017/S0950268809002295.Thompson, R.C., Palmer, C.S., O’Handley, R. (2008). The public health and clinical significance of Giardia and Cryptosporidium in domestic animals. Vet J, 177(1), 18-25. DOI:10.1016/j.tvjl.2007.09.022Toplu, S., Ural, K., Aysul, N., Ayan, A., Gültekin, M., Balıkçı, C. (2016). Hypomagnesaemia In Naturally Infected Calves with Giardia Spp. Kocatepe Vet J, 9(4), 386-390. DOI: 10.5578/kvj.37335Ural, K., Aysul, N., Voyvoda, H., Ulutas, B., Aldemir, O.S., Eren, H. (2014). Single dose of secnidazole treatment against naturally occuring Giardia duodenalis infection in Sakiz lambs. Rev MVZ Cordoba, 19(1), 4023-4032. DOI:10.21897/rmvz.122Ural, K., Gultekin, M., Pasa, S., Aysul, N., Ayan, A. (2017). Chloroquine treatment against naturally occuring Giardia duodenalis infection in dogs. Med Weter, 73(5), 280-283. DOI: 10.21521/mw.5695