Clinical, pathological and real time rt-pcr diagnosis of canine distemper virus infection in dogs

Year 2021, , 57 - 64, 31.08.2021

Nevin Tuzcu , Mehmet Tuzcu , Gökhan Akçakavak , Onur Başbuğ , Mehmet Kurul

https://doi.org/10.31797/vetbio.897795

Cited By: 2

Abstract

It was aimed to compare the Real Time RT-PCR based on nucleic acid detection and the histopathological method for viral nucleic acid detection by using the Real Time RT-PCR method for rapid diagnosis of the disease in both swab samples and postmortem tissue samples in dogs with canine distemper virus (CDV) infection. The material of study was composed of brain, cerebellum, liver, lung, spleen, kidney, sole pad, blood and swab taken from tonsil, nose and eye samples of dogs with clinical signs of fever, tear discharge and respiratory, digestive and nervous system infections, who started treatment with a clinical diagnosis of CDV infection, but died without responding to treatment, but were diagnosed with CDV according to histopathological findings. When the presence of viral nucleic acid was examined in swab samples taken during necropsy from dogs with intracytoplasmic and intranuclear inclusion bodies that are considered characteristic for CDV infection in histopathological examinations, it was observed that CDV nucleic acid copies were detected in all swabs taken from tonsils, in two of samples taken from the nose and conjunctiva, and viral nucleic acid copies were not detected in blood samples. Although clinical findings in dogs suggest the disease, virological and histopathological examinations used for definitive diagnosis take a long time. This situation brings PCR methods to the fore based on the reproduction of viral nucleic acids, and increases the importance of Real Time RT-PCR applications that minimize operator error, give fast results and can predict the number of viruses among PCR methods.

Keywords

Canine distemper virus (CDV), histopathology, real time rt-pcr

Köpeklerde canine distemper virüs enfeksiyonunun klinik, patolojik ve real-time RT-PCR ile teşhisi

Abstract

Klinik olarak canine distemper virüs (CDV) teşhisi konulan köpeklerde enfeksiyonun hem sürüntü örneklerinde ve hem de postmortem alınan doku örneklerinde hızlı teşhisine yönelik real-time RT-PCR metodu çalışılarak, viral nükleik asit tespitine dayalı real-time RT-PCR metodu ile histopatolojik yöntemin karşılaştırması amaçlanmıştır. Çalışmanın materyalini, ateş, gözyaşı akıntısı ile solunum, sindirim ve sinir sistemi enfeksiyonlarına ait klinik bulgulara sahip, CDV enfeksiyonu klinik tanısı ile tedaviye başlanmış, ancak tedaviye yanıt vermeden ölen köpeklerden histopatolojik bulgulara göre CDV teşhisi konulmuş köpeklere ait beyin, beyincik, karaciğer, akciğer, dalak, böbrek, taban yastığı ve kan ile tonsil, burun ve gözden alınan sürüntü örnekleri oluşturdu. Histopatolojik incelemelerde CDV enfeksiyonu için karakteristik sayılan intrasitoplazmik ve intranükleer inklüzyon cisimciği belirlenen köpeklerden nekropsi sırasında alınmış sürüntü örneklerinde viral nükleik asit varlığı incelendiğinde tonsillerden alınan sürüntülerin tamamında, burun ve konjunktivadan alınan örneklerin iki tanesinde CDV nükleik asit kopyalarının belirlendiği, kan örneklerinde ise viral nükleik asit belirlenemediği görüldü. Köpeklerde görülen klinik bulgular hastalığı düşündürse de kesin teşhis için kullanılan virolojik ve histopatolojik incelemeler uzun zaman almaktadır. Bu durum viral nükleik asitlerin çoğaltılması esasına dayalı PCR yöntemlerini ön plana çıkarmakta, PCR yöntemleri içerisinde de uygulayıcı hatasını minimize eden, hızlı sonuç veren ve virüs sayısının tahmin edilebildiği real-time RT-PCR uygulamalarının önemini artırmaktadır.

Keywords

: Canine distemper virüs (CDV), histopatoloji, real-time RT-PCR

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