Isolation and Identification of Yeasts in White Cheese

Abstract

In this study, firstly yeast was isolated from 50 white cheese samples produced by traditional methods from raw milk. The yeast species were isolated from 50 traditional white cheese samples and identified with the VITEK2 Compact system. There were obtained from white cheese samples 90 isolates 35.6% Candida sake (32), 13.3% Candida zeylanoides (12), 8.9% Candida famata (8) and 8.9% Candida kefyr (8) and the remaining 33.3% Candida sphaerica (4), Candida colliculasa (2), Candida boidinii (2), Candida lusitaniae (4), Candida parapsilosis (2), Candida sphaerica (4) Cryptococcus laurentii (4), Candida krusei (4) and Saccharomyces cerevisiae (4). Yeasts are known as a saprophyte found in many foods, but that some of them positively contribute to the fermentation. More over some yeasts are could used of starter culture during the manufacture of certain dairy product for maturation by supporting the functions. some yeasts species were isolated in this study, could be used as support culture.

Keywords

• Candida spp.
• Vitek2 compact system
• white cheese
• Yeast

References

1. Atanassova, M., Fernández-Otero, C., Rodríguez-Alonso, P., Fernández-No, I., Garabal, J., & Centeno, J. (2016). Characterization of yeasts isolated from artisanal short-ripened cows' cheeses produced in galicia (nw spain). Food Microbiology, 53, 172-181.
2. Besancon, X., Smet, C., Chabalier, C., Rivemale, M., Reverbel, J., Ratomahenina, R., & Galzy, P. (1992). Study of surface yeast flora of roquefort cheese. International Journal of Food Microbiology, 17(1), 9-18.
3. Callon, C., Delbes, C., Duthoit, F., & Montel, M. C. (2006). Application of sscp-pcr fingerprinting to profile the yeast community in raw milk salers cheeses. Syst Appl Microbiol, 29(2), 172-180. doi:10.1016/j.syapm.2005.07.008.
4. Cardozo, M. C., Fusco, Á. J., & Carrasco, M. S. (2017). Yeast microbiota in artisanal cheeses from corrientes, argentina. Revista Argentina de microbiologia, 50(2), 165-172.
5. Cosentino, S., Fadda, M. E., Deplano, M., Mulargia, A. F., & Palmas, F. (2001). Yeasts associated with sardinian ewe's dairy products. International Journal of Food Microbiology, 69(1-2), 53-58. doi:Doi 10.1016/S0168-1605(01)00572-4.
6. Dos Santos, M. T. P. G., Benito, M. J., de Guía Córdoba, M., Alvarenga, N., & de Herrera, S. R.-M. S. (2017). Yeast community in traditional portuguese serpa cheese by culture-dependent and-independent DNA approaches. International journal of food microbiology, 262, 63-70.
7. El-Sharoud, W. M., Belloch, C., Peris, D., & Querol, A. (2009). Molecular identification of yeasts associated with traditional egyptian dairy products. Journal of Food Science, 74(7), M341-M346. doi:DOI 10.1111/j.1750-3841.2009.01258.x.
8. Facchin, S., Barbosa, A. C., Carmo, L. S., Silva, M. C. C., Oliveira, A. L., Morais, P. B., & Rosa, C. A. (2013). Yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. Brazilian Journal of Microbiology, 44(3), 701-707.

9. Fadda, M. E., Cosentino, S., Deplano, M., & Palmas, F. (2001). Yeast populations in sardinian feta cheese. International Journal of Food Microbiology, 69(1–2), 153-156. doi:http://dx.doi.org/10.1016/S0168-1605(01)00586-4.
10. Fatichenti, F., Bergere, J. L., Deiana, P., & Farris, G. A. (1983). Antagonistic activity of debaryomyces hansenii towards clostridium tyrobutyricum and cl. Butyricum. Journal of dairy research, 50(4), 449-457.
11. Ferreira, A. D., & Viljoen, B. C. (2003). Yeasts as adjunct starters in matured cheddar cheese. International Journal of Food Microbiology, 86(1-2), 131-140. doi:Doi 10.1016/S0168-1605(03)00252-6.
12. Fleet, G. H. (1990). Yeasts in dairy-products. Journal of Applied Bacteriology, 68(3), 199-211. doi:DOI 10.1111/j.1365-2672.1990.tb02566.x.
13. Fröhlich‐Wyder, M. T., Arias‐Roth, E., & Jakob, E. (2019). Cheese yeasts. Yeast, 36(3), 129-141.
14. Gadaga, T. H., Mutukumira, A. N., & Narvhus, J. A. (2001). Growth characteristics of candida kefyr and two strains of lactococcus lactis subsp lactis isolated from zimbabwean naturally fermented milk. International Journal of Food Microbiology, 70(1-2), 11-19. doi:Doi 10.1016/S0168-1605(01)00501-3.
15. Garnier, L., Valence, F., & Mounier, J. (2017). Diversity and control of spoilage fungi in dairy products: An update. Microorganisms, 5(3), 33. doi:10.3390/microorganisms5030042.
16. Haastrup, M. K., Johansen, P., Malskær, A. H., Castro-Mejía, J. L., Kot, W., Krych, L., . . . Jespersen, L. (2018). Cheese brines from danish dairies reveal a complex microbiota comprising several halotolerant bacteria and yeasts. International journal of food microbiology, 285, 173-187.
17. Helmy, E., Soliman, S., Abdel-Ghany, T. M., & Ganash, M. (2019). Evaluation of potentially probiotic attributes of certain dairy yeast isolated from buffalo sweetened karish cheese. Heliyon, 5(5), e01649.
18. Jakobsen, M., & Narvhus, J. (1996). Yeasts and their possible beneficial and negative effects on the quality of dairy products. International Dairy Journal, 6(8-9), 755-768. doi:Doi 10.1016/0958-6946(95)00071-2.
19. Kaminarides, S. E., & Laskos, N. S. (1992). Yeasts in factory brine of feta cheese. Aust. J. Dairy Technol., 47 (1) 68–71.
20. Karasu-Yalcin, S., Senses-Ergul, S., & Ozbas, Z. Y. (2017 ). Enzymatic characterization of yeast strains originated from traditional mihalic cheese. J Microbiol Biotech Food Sci 6 (5) 1152-1156.
21. Kesenkaş, H., & Akbulut, N. (2006). Destek kültür olarak kullanılan bazı mayaların beyaz peynir aroması üzerine etkileri. Ege Üniv. Ziraat Fak. Derg, 43(2), 73-84.
22. Lopandic, K., Zelger, S., Bánszky, L. K., Eliskases-Lechner, F., & Prillinger, H. (2006). Identification of yeasts associated with milk products using traditional and molecular techniques. Food Microbiology, 23(4), 341-350. doi:http://dx.doi.org/10.1016/j.fm.2005.05.001.
23. Ozmen Togay, S., Capece, A., Siesto, G., Aksu, H., Sandikci Altunatmaz, S., Yilmaz Aksu, F., . . . Karagul Yuceer, Y. (2020). Molecular characterization of yeasts isolated from traditional turkish cheeses. Food Science and Technology(AHEAD).
24. Padilla, B., Belloch, C., Lopez-Diez, J. J., Flores, M., & Manzanares, P. (2014). Potential impact of dairy yeasts on the typical flavour of traditional ewes' and goats' cheeses. International Dairy Journal, 35(2), 122-129. doi:DOI 10.1016/j.idairyj.2013.11.002
25. Pereira-Dias, S., Potes, M. E., Marinho, A., Malfeito-Ferreira, M., & Loureiro, V. (2000). Characterisation of yeast flora isolated from an artisanal portuguese ewes' cheese. International Journal of Food Microbiology, 60(1), 55-63. doi:Doi 10.1016/S0168-1605(00)00323-8.
26. Pincus, D. H. (2006). Microbial identification using the biomerieux vitek® 2 system. Encyclopedia of Rapid Microbiological Methods. Bethesda, MD: Parenteral Drug Association.
27. Romano, P., Ricciardi, A., Salzano, G., & Suzzi, G. (2001). Yeasts from water buffalo mozzarella, a traditional cheese of the mediterranean area. International Journal of Food Microbiology, 69(1-2), 45-51.
28. Seiler, H., & Busse, M. (1990). The yeasts of cheese brines. International Journal of Food Microbiology, 11(3-4), 289-303.
29. Van Den Tempel, T., & Jakobsen, M. (2000). The technological characteristics of debaryomyces hansenii and yarrowia lipolytica and their potential as starter cultures for production of danablu. International Dairy Journal, 10(4), 263-270. doi:http://dx.doi.org/10.1016/S0958-6946(00)00053-4
30. Welthagen, J. J., & Viljoen, B. C. (1997). The value of certain chemotaxonomic methods in the identification of food related yeasts. Food Microbiology, 14(3), 231-245. doi:DOI 10.1006/fmic.1996.0087
31. Welthagen, J. J., & Viljoen, B. C. (1998). Yeast profile in gouda cheese during processing and ripening. International Journal of Food Microbiology, 41(3), 185-194. doi:http://dx.doi.org/10.1016/S0168-1605(98)00042-7
32. Welthagen, J. J., & Viljoen, B. C. (1999). The isolation and identification of yeasts obtained during the manufacture and ripening of cheddar cheese. Food Microbiology, 16(1), 63-73. doi:DOI 10.1006/fmic.1998.0219
33. Westall, S., & Filtenborg, O. (1998). Yeast occurrence in danish feta cheese. Food Microbiology, 15(2), 215-222. doi:http://dx.doi.org/10.1006/fmic.1997.0161