Natural Cyst Shedding in Calves Infected with Giardia Duodenalis

Year 2016, Volume: 1 Issue: 1, 14 - 19, 11.12.2016

Adnan Ayan Kerem Ural , Nuran Aysul Mehmet Gültekin Hasan Erdoğan Canberk Balıkçı , Songül Toplu Göktuğ Toros

Abstract

The
present study was carried out on field conditions, involving 9 diarrhetic
calves naturally ınfected with Giardia duodenalis in different districts of Aydın
region. Precise diagnosis has confirmed by Nested PCR analysis of cases, to
these of which in immunochromatographic rapid diagnostic test kits revealed G. duodenalis antigens as regarding cyst
excretion. Following cysts count in gram stool (Macmaster method), the
geometric means on days  0., 3., 7. ve
10.  were determined 18412.60; 10504.26;
17675.21 ve 10767.17 respectively. Initial values (day 0) compared with the
3rd, 7th days revealed in significant reduction, besides on day 7, in
comparison to 3rd and 10th days, insignificant increase. All fecal samples were
deemed positive with Nested PZR analysis. According to present authours
knowledge this study carried out in calves infected with G. duodenalis via PCR, determination of natural cyst shedding may
provide future directions for 
arasitological, clinical and molecular studies. And also it was
concluded that the latter study may provide valuable contribution to the
regional infection map. It must also be taken into account G. duodenalis as a forgotten etiological agent, involved in calf
diarrhea causing economical loss in our country.

Keywords

G. duodenalis, calf, Nested PZR

Giardia Duodenalis ile İnfekte Buzağılarda Doğal Kist Saçılımı

Abstract

Bu çalışma saha koşullarında Aydın
yöresindeki farklı ilçelerde ishalli ve Giardia duodenalis  ile
doğal enfekte 9 buzağıda gerçekleştirildi. İmmunokromotografik hızlı diagnostik
test kitleri ile G. duodenalis antijenleri belirlenen olgular
Nested PZR analizine tabi tutularak tanı kesinleştirildi. Gram dışkıda kist
sayımları (Macmaster metodu) sonrası 0., 3., 7. ve 10. günlerde kist
sayımlarının geometrik ortalama sırasıyla 18412.60; 10504.26; 17675.21 ve
10767.17 adet olduğu belirlendi. Kist açılımı ele alındığında başlangıç
değerlerine (0. gün) göre 3., 7. ve 10 günlerde istatistiksel önemi bulunmayan
bir azalmanın gerçekleştiği; 7. günde 3. ve 10. günlere kıyasla istatistiksel
anlamı bulunmayan bir artışın gözlemlendiği dikkati çekti. Nested PZR
analizinde tüm dışkı örneklerinin pozitif olduğu tespit edildi. Yazarların
bilgisine göre Ege Bölgesindeki G. doudenalis ile doğal infekte buzağılarda PZR eşliğinde
gerçekleştirilen bu çalışmada, doğal kist saçılımının tespit edilmesiyle
ileride gerçekleştirilecek parazitolojik, klinik ve moleküler çalışmalara yön
verebileceği düşünüldü. İlaveten anılan çalışmanın bölge enfeksiyon haritasına
değerli katkı sağlayacağı kanaatine varıldı. Ülkemizde önemli ekonomik
kayıplara neden olan buzağı ishalleri içerisinde G. duodenalis’ in
unutulmuş etiyolojik etkenler arasında yer aldığı göz önünde bulundurulmalıdır.

Keywords

G. duodenalis, buzağı, Nested PZR

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