GPx, SOD, CAT enzyme activities and Vitamin A values in the Marmara and Black Sea Region Scad’s Liver
Abstract
The amount and number of pollutants causing marine pollution are increasing day by day. The use of antioxidant defense system components as biomarkers in aquatic species is preferred for early detection of contamination. In this study, the activities of GPx, SOD and CAT enzymes and vitamin A status in the liver of scad (Trachurus trachurus) from the Black Sea and Marmara Sea pelagic fishes were investigated. Samples were collected march (1st period) and November (2nd period). Compared to the Marmara Sea scads, the second term GPx activity was increased according to the 1st rotation (p ≤ 0.01) and the 2nd Term vitamin A level was found to be lower than the 1st rotation (p ≤ 0,05). In the comparison of Marmara and the Black Sea, GPx activity was found higher in Black Sea scad’s compared to Marmara (p ≤ 0.05). CAT activity and vitamin A level in both periods was
lower in the Black Sea samples than in Marmara (CAT:1st period p ≤ 0,01, 2nd
period p ≤ 0,05; vitamin A:1st period p ≤ 0,05, 2nd period p ≤ 0,01). Antioxidant system parameters, which are biomonitor
of pollution in marine fishes, the study of pelagic fish between carnivorous
and herbivorous species, between surface and bottom fish species, migratory
fishes and nonimmigrant sea fish both within and between them will enable us to
obtain more detailed information about the antioxidant system of our marine
fishes and to consume healthier seafood. In this study, the relationship
between marine pollution and pelagic fish's antioxidant metabolism has been
investigated and in terms of the obtained data, it can be a source of work to
be done in this respect.
References
- Ait Alla, A., Mouneyrac, C., Durou, C., Moukrim, A., Pellerin, J.(2006): Tolerance and biomarkers as useful tools for assessing environmental quality in the Oued Souss estuary (Bay of Agadir, Morocco). Comparative Biochemistry and Physiology, Part C 143: 23-29. Anonim (2000). Ulusal Çevre Eylem Planı (UÇEP) İhtisas Komisyon Raporu. T.C. Çevre Bakanlığı Yayınları 2-91.Atay, D., Pulatsü, S. (2000). Su Kirlenmesi ve Kontrolü. Ankara Üniversitesi Ziraat Fakültesi Su Ürünleri Bölümü Yayın No:1513: 1-178. Barsiene, J., Lehtonen, K.K., Koehler, A., Broeg, K., Vuorinen P.J., Lang, T., Pempkowiak, J., Syvokiene, J., Dedonyte, V., Rybakovas, A., Repecka, R., Vuontisjarvi, H., Kopecka, J. (2006): Biomarker responses in flounder (Platichythys flesus) and mussel (Mytilus edulis) in the Klaipeda-Bütinge area (Baltic Sea). Marine Pollution Bulletin in press.Benli, A.H. (2004). Balıkcılık ve Yetiştiricilik.Erişim adresi: http://stu.inonu.edu.tr/cevre/balikcilik.htm Erişim tarihi: 12.05.2004Borresen, T. (1995). Muscle Anatomy and Function Chemical Composition. FAO, 348:24-27.Cheung, C. C. C., Zheng, G. J., Lİ, a. M. Y., Richardson, B. J., Lam, P. K. S. (2001). Relationship between tissue concentrations of polycylic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquatic Toxicol. 52, 189-203.Esin, A., Ekni, M., Gamgam, H. (1997). Sağlık Bilimlerinde İstatistik. Gazi Üniversitesi Yayın No: 171: 355-365, Ankara.Fenet, H., Casellas, C., Bontoux, J. (1998). Laboratory and Field-Caging Studies on Hepatic Enzymatic Activites in European Eel and Rainbow Trout. Ecotox. And Env. Safety, 40: 137-143.Filho, W. (1996). Fish antioxidant defenses-A comparative approach. Brazilian J. Med. and Biol. Res. 29: 1735-1742.Gabryelak, T., Pıatkowska, M., Leyko, W., Peres, G. (1983). Seasonal Variations in The Activities of Peroxide Metabolism Enzymes in Erythrocytes of Freshwater Fish Species. Comp. Biochem. Physiol, 75.383-385.Garrigues, Narbonne, J.F., Lafaure, M., Ribera, D., Lemaire, P., Raoux, C., Michel, X., Salaun, J.P., Monad, J.L., Romeo, M. (1993). Banking of environmental samples for short-term biochemical and chemical monitoring of organic contamination in coastal marine environments; the GICBEM experience (1986-1990). The Science of the Total Environment 139/140, 225-236.Grys, S. (1980). Indirect spectrophometry on vitamin A products. Peak signal readout. Methods in Enzymology, 67: 195-199.İmre, S., Sağlık, S. (1998). Fatty Acid Composition and Cholesterol Content of Some Turkish Fish Species. Turk J. Chem., 22: 321-324.Kolaylı, S., Keha, E. (1999). A Comparative Study of Antioxidant Enzyme Activities in Freshwater and Seawater-Adapted Rainbow Trout. J. Biochem. Molecular Toxicology, 13: 334-337.Lehtonen, K.K., Schiede, D., Köpler, A., Lang, T., Vuorinen P.J., Förlin, L, Barsiene, J., Pempkowiak, J., Gercken, J. (2006): The BEEP project in the Baltic Sea: Overview of results and outline for a regional biological effects monitoring strategy. Marine Pollution Bulletin in press.Napierska, D., Podolska, M. (2005): Biomarkers of contaminant exposure: results of a field study with flounder (Platichythys flesus) from the southern Baltic Sea. Marine Pollution Bulletin 50: 758-767.Narbonne, J.F., Aarab, N., Clerandeau, C., Daubeze, M., Narbonne, J., Champeau, O., Garrigues, P. (2005): Scale of classification based on biochemical markers in mussels: application to pollution monitoring in Mediterranean coatsts and temporal trends. Biomarkers, 10 (1): 58-71.Oost, R., Heida, H., Opperhuizen, A., Vermeulens, N. (1991). İnterrelationships Between Bioaccumulation of Organic Trace Pollutants and MFO-İnduction in Fish. Comp. Biochem. Physiol., 100C: 43-47.Orbea, A., Fahimi, D.H., Cajaravile, M.P. (2000). Immunolocalization of four antioxidant enzymes in digestive glands of mollusks and crustaceans and fish liver. Histochem. Cell. Biol. 114: 393-404.Palace, V.P., Brown, S.B., Baron, C.L., Fitzsimons, J., Woodin, R.B., Stegeman, J.J., Klaverkamp, J.F. (1998). An evaluation of the relationships among oxidative stress, antioxidant vitamins and early mortality syndrome (EMS) of lake trout (Salvelinus namaycush) from lake ontario. Aquatic Toxicol. 43: 195-208.Sarkar, A., Ray, D., Shrivastava Amulya, N.(2006) : Molecular Biomarkers: Their significanve and application in marine pollution monitoring. Ecotoxicology. 15(4):333-40.Suzuki, J.P., Katoh, N.A. (1990). A simple and cheap method for measuring serum vitamin A in catltle using only spectrophotometer. J. Vet. Sci., 52: 1281-1283Yücel, Ş., Erkoyuncu, İ. (2000). Orta Karadeniz Bölgesi’inde Avlanan İstavrit (Trachurus trachurus)’in Populasyon Dinamiği. Turk. J. Biol., 24:543-552.Zorita, I., Strogyloudi, E., Buxens, A., Mazon, L.I., Papathanassiou, E., Soto, M., Cajaraville, M.P. (2005): Application of two SH-based methods for metallothionein determination in mussels and intercalibration of the spectrophotometric method: laboratory and field studies in the Mediterranean Sea.
Marmara ve Karadeniz Bölgesi istavrit karaciğerinde GPx, SOD, CAT enzim aktiviteleri ve vitamin A değerleri
Abstract
Deniz
kirliliğine sebep olan kirleticilerin miktarı ve sayısı her geçen gün daha da
artmaktadır. Akuatik türlerde biyobelirteç olarak antioksidan savunma sistemi
elemanlarının kullanımı kirliliğin erken teşhisi amacıyla tercih edilmektedir.
Bu çalışmada Karadeniz ve Marmara denizi yüzey balıklarından istavrit (Trachurus trachurus) karaciğer
dokusunda GPx, SOD ve CAT enzim aktiviteleri ve vitamin A düzeyi
araştırılmıştır. Örnekler mart (1.dönem) ve kasım (2.dönem) aylarında
toplanmıştır. Marmara
Denizi istavrit balıkları dönemler arasındaki karşılaştırmasında 2. Dönem GPx
aktivitesi 1. Döneme göre artmış (p ≤ 0.01), 2. Dönem vitamin A düzeyi 1.
Döneme göre düşük (p ≤ 0,05) bulunmuştur. Marmara ile Karadeniz’in
karşılaştırılmasında GPx aktivitesi Karadeniz istavritlerinde Marmara’ya göre
yüksek (p ≤ 0,05) düzeyde tespit edilmiştir. Her iki dönemde de CAT aktivitesi
ve vitamin A düzeyi Karadeniz örneklerinde Marmara’ya göre düşük (CAT:1.Dönem p
≤ 0,01, 2. Dönem p ≤ 0,05; Vitamin A:1.Dönem p ≤ 0,05, 2. Dönem p ≤ 0,01)
tespit edilmiştir. Deniz
balıklarında kirliliğin biyomönütörü olan antioksidan sistem parametrelerinin;
yüzey balıklarının karnivor ve herbivor türleri arasında, yüzey ve dip balık
türleri arasında, göçmen balıklar ile göçmen olmayan deniz balıkları hem kendi
içlerinde hem de aralarında çalışılması, Deniz balıkları antioksidan sistemi
hakkında daha detaylı bilgi edinilmesine ve daha sağlıklı su ürünü
tüketilmesine olanak sağlayacaktır. Bu çalışmada, deniz kirliliği ile yüzey
balıklarının antioksidan metabolizması ilişkisi incelenmiştir.Elde edilen
veriler, bu konuda yapılacak çalışmalara kaynak oluşturabilir.
References
- Ait Alla, A., Mouneyrac, C., Durou, C., Moukrim, A., Pellerin, J.(2006): Tolerance and biomarkers as useful tools for assessing environmental quality in the Oued Souss estuary (Bay of Agadir, Morocco). Comparative Biochemistry and Physiology, Part C 143: 23-29. Anonim (2000). Ulusal Çevre Eylem Planı (UÇEP) İhtisas Komisyon Raporu. T.C. Çevre Bakanlığı Yayınları 2-91.Atay, D., Pulatsü, S. (2000). Su Kirlenmesi ve Kontrolü. Ankara Üniversitesi Ziraat Fakültesi Su Ürünleri Bölümü Yayın No:1513: 1-178. Barsiene, J., Lehtonen, K.K., Koehler, A., Broeg, K., Vuorinen P.J., Lang, T., Pempkowiak, J., Syvokiene, J., Dedonyte, V., Rybakovas, A., Repecka, R., Vuontisjarvi, H., Kopecka, J. (2006): Biomarker responses in flounder (Platichythys flesus) and mussel (Mytilus edulis) in the Klaipeda-Bütinge area (Baltic Sea). Marine Pollution Bulletin in press.Benli, A.H. (2004). Balıkcılık ve Yetiştiricilik.Erişim adresi: http://stu.inonu.edu.tr/cevre/balikcilik.htm Erişim tarihi: 12.05.2004Borresen, T. (1995). Muscle Anatomy and Function Chemical Composition. FAO, 348:24-27.Cheung, C. C. C., Zheng, G. J., Lİ, a. M. Y., Richardson, B. J., Lam, P. K. S. (2001). Relationship between tissue concentrations of polycylic aromatic hydrocarbons and antioxidative responses of marine mussels, Perna viridis. Aquatic Toxicol. 52, 189-203.Esin, A., Ekni, M., Gamgam, H. (1997). Sağlık Bilimlerinde İstatistik. Gazi Üniversitesi Yayın No: 171: 355-365, Ankara.Fenet, H., Casellas, C., Bontoux, J. (1998). Laboratory and Field-Caging Studies on Hepatic Enzymatic Activites in European Eel and Rainbow Trout. Ecotox. And Env. Safety, 40: 137-143.Filho, W. (1996). Fish antioxidant defenses-A comparative approach. Brazilian J. Med. and Biol. Res. 29: 1735-1742.Gabryelak, T., Pıatkowska, M., Leyko, W., Peres, G. (1983). Seasonal Variations in The Activities of Peroxide Metabolism Enzymes in Erythrocytes of Freshwater Fish Species. Comp. Biochem. Physiol, 75.383-385.Garrigues, Narbonne, J.F., Lafaure, M., Ribera, D., Lemaire, P., Raoux, C., Michel, X., Salaun, J.P., Monad, J.L., Romeo, M. (1993). Banking of environmental samples for short-term biochemical and chemical monitoring of organic contamination in coastal marine environments; the GICBEM experience (1986-1990). The Science of the Total Environment 139/140, 225-236.Grys, S. (1980). Indirect spectrophometry on vitamin A products. Peak signal readout. Methods in Enzymology, 67: 195-199.İmre, S., Sağlık, S. (1998). Fatty Acid Composition and Cholesterol Content of Some Turkish Fish Species. Turk J. Chem., 22: 321-324.Kolaylı, S., Keha, E. (1999). A Comparative Study of Antioxidant Enzyme Activities in Freshwater and Seawater-Adapted Rainbow Trout. J. Biochem. Molecular Toxicology, 13: 334-337.Lehtonen, K.K., Schiede, D., Köpler, A., Lang, T., Vuorinen P.J., Förlin, L, Barsiene, J., Pempkowiak, J., Gercken, J. (2006): The BEEP project in the Baltic Sea: Overview of results and outline for a regional biological effects monitoring strategy. Marine Pollution Bulletin in press.Napierska, D., Podolska, M. (2005): Biomarkers of contaminant exposure: results of a field study with flounder (Platichythys flesus) from the southern Baltic Sea. Marine Pollution Bulletin 50: 758-767.Narbonne, J.F., Aarab, N., Clerandeau, C., Daubeze, M., Narbonne, J., Champeau, O., Garrigues, P. (2005): Scale of classification based on biochemical markers in mussels: application to pollution monitoring in Mediterranean coatsts and temporal trends. Biomarkers, 10 (1): 58-71.Oost, R., Heida, H., Opperhuizen, A., Vermeulens, N. (1991). İnterrelationships Between Bioaccumulation of Organic Trace Pollutants and MFO-İnduction in Fish. Comp. Biochem. Physiol., 100C: 43-47.Orbea, A., Fahimi, D.H., Cajaravile, M.P. (2000). Immunolocalization of four antioxidant enzymes in digestive glands of mollusks and crustaceans and fish liver. Histochem. Cell. Biol. 114: 393-404.Palace, V.P., Brown, S.B., Baron, C.L., Fitzsimons, J., Woodin, R.B., Stegeman, J.J., Klaverkamp, J.F. (1998). An evaluation of the relationships among oxidative stress, antioxidant vitamins and early mortality syndrome (EMS) of lake trout (Salvelinus namaycush) from lake ontario. Aquatic Toxicol. 43: 195-208.Sarkar, A., Ray, D., Shrivastava Amulya, N.(2006) : Molecular Biomarkers: Their significanve and application in marine pollution monitoring. Ecotoxicology. 15(4):333-40.Suzuki, J.P., Katoh, N.A. (1990). A simple and cheap method for measuring serum vitamin A in catltle using only spectrophotometer. J. Vet. Sci., 52: 1281-1283Yücel, Ş., Erkoyuncu, İ. (2000). Orta Karadeniz Bölgesi’inde Avlanan İstavrit (Trachurus trachurus)’in Populasyon Dinamiği. Turk. J. Biol., 24:543-552.Zorita, I., Strogyloudi, E., Buxens, A., Mazon, L.I., Papathanassiou, E., Soto, M., Cajaraville, M.P. (2005): Application of two SH-based methods for metallothionein determination in mussels and intercalibration of the spectrophotometric method: laboratory and field studies in the Mediterranean Sea.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | August 31, 2018 |
| Submission Date | August 6, 2018 |
| Acceptance Date | August 30, 2018 |
| Published in Issue | Year 2018 Volume: 3 Issue: 2 |
