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Çeşitli zayıf organik asitler ve kombinasyonlarının saccharomyces cerevisiae'ye karşı antifungal etkileri

Year 2018, Volume: 3 Issue: 3, 28 - 34, 31.12.2018
https://doi.org/10.31797/vetbio.451505

Abstract

Zayıf organik asitlerin S. cerevisiae hücrelerine karşı antifungal etkinliği incelenmiştir. Zayıf organik asitler olarak, hekzanoik (C6), oktanoik (C8), dekanoik (C10) ve benzoik asitlerin Minimum İnhibitör Konsantrasyon (MİK) ve inhibisyon bölgesi ölçümleri ile belirlenmiştir. MİK sonuçlarına göre maya hücrelerine karşı en etkili asit, dekanoik asittir (MİK: 0,2-0,3 mM). Bahsi geçen zayıf asitlerin inhibisyon mekanizmalarını anlayabilmek için, ekstraselüler ortam pH ölçümleri yapılmıştır. Ekstraselüler pH’daki düşüş; pH’da aynı miktarda düşüşe neden olan hidroklorik asit (HCl) ile karşılaştırılmıştır. Sonuçlar, maya hücrelerine karşı zayıf asitlerin etkinliklerinin sadece asitlikten kaynaklı olmadığını, ancak anyonların toksik etkisi ve zayıf asitlerin hücresel membran içine sızmasının rol oynayabileceğini göstermiştir. Buna ek olarak, zayıf asitlerin sinerjik etkileri incelenmiş ve bu zayıf asitlerin kombinasyolarının tek başına kullanımlara göre daha etkili olduğu gözlemlenmiştir. Çalışmada, zayıf asitlerin antifungal aktivite mekanizmalarına genel olarak bir açıklama getirmekle birlikte farklı konsantrasyonlarda kombinasyon halinde kullanımları da incelenmiştir.

References

  • Alexandre, H., Mathieu, B., Charpentier, C. (1996). Alteration in membrane fluidity and lipid composition, and modulation of H+-ATPase activity in Saccharomyces cerevisiae caused by decanoic acid, Microbiology, 142(3),469-475.
  • Bauer, A., Kirby, W., Sherris, J.C., Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method, American Journal of Clinical Pathology, 45(7),493.
  • Bracey, D., Holyoak, C., Coote, P. (1998). Comparison of the inhibitory effect of sorbic acid and amphotericin B on Saccharomyces cerevisiae: is growth inhibition dependent on reduced intracellular pH?, Journal of Applied Microbiology, 85(6),1056-1066.
  • Bergsson, G., Arnfinnsson, J., Steingrı́msson, Ó., Thormar, H. (2001). In vitro killing of Candida albicans by fatty acids and monoglycerides, Antimicrobial Agents and Chemotherapy, 45(11),3209-3212.
  • Cabral, M.G., Viegas, C.A., Sá-Correia, I. (2001). Mechanisms underlying the acquisition of resistance to octanoic-acid-induced-death following exposure of Saccharomyces cerevisiae to mild stress imposed by octanoic acid or ethanol, Archives of Microbiology, 75(4),301-307.
  • Gášková, D., Plášek, J., Zahumenský, J., et al. (2013). Alcohols are inhibitors of Saccharomyces cerevisiae multidrug-resistance pumps Pdr5p and Snq2p, FEMS Yeast Research, 13(8),782-795.
  • Hatzixanthis, K., Mollapour, M., Seymour, I., et al. (2003). Moderately lipophilic carboxylate compounds are the selective inducers of the Saccharomyces cerevisiae Pdr12p ATP‐binding cassette transporter, Yeast, 20(7),575-585.
  • Hazan, R., Levine, A., Abeliovich, H. (2004). Benzoic acid, a weak organic acid food preservative, exerts specific effects on intracellular membrane trafficking pathways in Saccharomyces cerevisiae, Applied and Environmental Microbiology 70(8),4449-4457.
  • Holyoak, C.D., Bracey, D., Piper, P.W., Kuchler, K., Coote P.J. (1999). The Saccharomyces cerevisiae weak-acid-inducible ABC transporter Pdr12 transports fluorescein and preservative anions from the cytosol by an energy-dependent mechanism, Journal of Bacteriology 181(15),4644-4652.
  • Jarboe, L.R., Liu, P., Royce, L.A. (2011). Engineering inhibitor tolerance for the production of biorenewable fuels and chemicals, Current Opinion in Chemical Engineering 1(1), 38-42.
  • Kumar, A., Singh, S., Jain, S., Kumar, P. (2011). Synthesis, antimicrobial evaluation, QSAR and in silico admet studies of decanoic acid derivatives, Act Pol Pharm, 68:191-204.
  • Kumar, T.A., Bukvicki, D., Gottardi, D., et al. (2014). Eucalyptus essential oil as a natural food preservative: in vivo and in vitro antiyeast potential, BioMed Research International.
  • Lambert, R., Stratford, M. (1999). Weak‐acid preservatives: modelling microbial inhibition and response, Journal of Applied Microbiology, 86(1),157-164. Legras, J., Erny, C., Le, Jeune, C., et al. (2011). Activation of two different resistance mechanisms in Saccharomyces cerevisiae upon exposure to octanoic and decanoic acids, Applied and Environmental Microbiology, 76(22),7526-7535.
  • Mollapour, M., Phelan, J.P., Millson, S.H., Piper P.W., Cooke F.T. (2006). Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae. Biochemical Journal, 395(1),73-80.
  • Papadimitriou, M.N., Resende, C., Kuchler, K., Brul, S. (2007). High Pdr12 levels in spoilage yeast (Saccharomyces cerevisiae) correlate directly with sorbic acid levels in the culture medium but are not sufficient to provide cells with acquired resistance to the food preservative, International Journal of Food Microbiology, 113(2),173-179.
  • Rattanachaikunsopon, P., Phumkhachorn, P. (2010). Lactic acid bacteria: their antimicrobial compounds and their uses in food production, Annals of Biological Research, 1,218-228.
  • Tenreiro, S., Nunes, PcA., Viegas, C.A., et al. (2002). AQR1 gene (ORF YNL065w) encodes a plasma membrane transporter of the major facilitator superfamily that confers resistance to short-chain monocarboxylic acids and quinidine in Saccharomyces cerevisiae, Biochemical and Biophysical Research Communications, 292(3),741-748.
  • Ullah, A., Orij, R., Brul, S., Smits, G.J. (2012). Quantitative analysis of the modes of growth inhibition by weak organic acids in Saccharomyces cerevisiae, Applied and Environmental Microbiology, 78(23), 8377-8387.
  • Viegas, C.A., Rosa, M.F., Sá-Correia, I., Novais, J.M. (1989). Inhibition of yeast growth by octanoic and decanoic acids produced during ethanolic fermentation, Applied and Environmental Microbiology, 55(1),21-2.
  • Viegas, C.A., Sá-Correia, I. (1997). Effects of low temperatures (9–33 C) and pH (3.3–5.7) in the loss of Saccharomyces cerevisiae viability by combining lethal concentrations of ethanol with octanoic and decanoic acids, International Journal of Food Microbiology, 34(3),267-277.
  • Wang, Y., Zeng, X., Zhou, Z., et al. (2015). Inhibitory effect of nerol against Aspergillus niger on grapes through a membrane lesion mechanism, Food Control, 55:54-61.

Antifungal activity of various weak organic acids and their combinations against saccharomyces cerevisiae

Year 2018, Volume: 3 Issue: 3, 28 - 34, 31.12.2018
https://doi.org/10.31797/vetbio.451505

Abstract

Antifungal activity of four weak organic acids against S. cerevisiae cells was examined. Antifungal effects of hexanoic (C6), octanoic (C8), decanoic (C10) and benzoic acids were determined through Minimum Inhibitory Concentration (MIC), and inhibition zone measurements. The most effective weak acid was decanoic acid (MIC: 0.2-0.3 mM) according to MIC results. In order to have some insight into the inhibition mechanism of these weak acids, their efficiency was compared with that of hydrochloric acid (HCl) giving the same amount of drop in extracellular pH. Results demonstrated that the inhibition of yeast cells by weak acids is not simply due to acidity, but toxic effect of the anion and the insertion of the weak acids inside the cellular membrane may play a role. Moreover, synergistic effects of weak acids were examined, and it has been shown that combinations of weak acids are more effective than using weak acids alone. Thus, this research not only opens new perspectives on antifungal activity mechanisms of weak acids but also help their usage in combination widely.

References

  • Alexandre, H., Mathieu, B., Charpentier, C. (1996). Alteration in membrane fluidity and lipid composition, and modulation of H+-ATPase activity in Saccharomyces cerevisiae caused by decanoic acid, Microbiology, 142(3),469-475.
  • Bauer, A., Kirby, W., Sherris, J.C., Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disk method, American Journal of Clinical Pathology, 45(7),493.
  • Bracey, D., Holyoak, C., Coote, P. (1998). Comparison of the inhibitory effect of sorbic acid and amphotericin B on Saccharomyces cerevisiae: is growth inhibition dependent on reduced intracellular pH?, Journal of Applied Microbiology, 85(6),1056-1066.
  • Bergsson, G., Arnfinnsson, J., Steingrı́msson, Ó., Thormar, H. (2001). In vitro killing of Candida albicans by fatty acids and monoglycerides, Antimicrobial Agents and Chemotherapy, 45(11),3209-3212.
  • Cabral, M.G., Viegas, C.A., Sá-Correia, I. (2001). Mechanisms underlying the acquisition of resistance to octanoic-acid-induced-death following exposure of Saccharomyces cerevisiae to mild stress imposed by octanoic acid or ethanol, Archives of Microbiology, 75(4),301-307.
  • Gášková, D., Plášek, J., Zahumenský, J., et al. (2013). Alcohols are inhibitors of Saccharomyces cerevisiae multidrug-resistance pumps Pdr5p and Snq2p, FEMS Yeast Research, 13(8),782-795.
  • Hatzixanthis, K., Mollapour, M., Seymour, I., et al. (2003). Moderately lipophilic carboxylate compounds are the selective inducers of the Saccharomyces cerevisiae Pdr12p ATP‐binding cassette transporter, Yeast, 20(7),575-585.
  • Hazan, R., Levine, A., Abeliovich, H. (2004). Benzoic acid, a weak organic acid food preservative, exerts specific effects on intracellular membrane trafficking pathways in Saccharomyces cerevisiae, Applied and Environmental Microbiology 70(8),4449-4457.
  • Holyoak, C.D., Bracey, D., Piper, P.W., Kuchler, K., Coote P.J. (1999). The Saccharomyces cerevisiae weak-acid-inducible ABC transporter Pdr12 transports fluorescein and preservative anions from the cytosol by an energy-dependent mechanism, Journal of Bacteriology 181(15),4644-4652.
  • Jarboe, L.R., Liu, P., Royce, L.A. (2011). Engineering inhibitor tolerance for the production of biorenewable fuels and chemicals, Current Opinion in Chemical Engineering 1(1), 38-42.
  • Kumar, A., Singh, S., Jain, S., Kumar, P. (2011). Synthesis, antimicrobial evaluation, QSAR and in silico admet studies of decanoic acid derivatives, Act Pol Pharm, 68:191-204.
  • Kumar, T.A., Bukvicki, D., Gottardi, D., et al. (2014). Eucalyptus essential oil as a natural food preservative: in vivo and in vitro antiyeast potential, BioMed Research International.
  • Lambert, R., Stratford, M. (1999). Weak‐acid preservatives: modelling microbial inhibition and response, Journal of Applied Microbiology, 86(1),157-164. Legras, J., Erny, C., Le, Jeune, C., et al. (2011). Activation of two different resistance mechanisms in Saccharomyces cerevisiae upon exposure to octanoic and decanoic acids, Applied and Environmental Microbiology, 76(22),7526-7535.
  • Mollapour, M., Phelan, J.P., Millson, S.H., Piper P.W., Cooke F.T. (2006). Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae. Biochemical Journal, 395(1),73-80.
  • Papadimitriou, M.N., Resende, C., Kuchler, K., Brul, S. (2007). High Pdr12 levels in spoilage yeast (Saccharomyces cerevisiae) correlate directly with sorbic acid levels in the culture medium but are not sufficient to provide cells with acquired resistance to the food preservative, International Journal of Food Microbiology, 113(2),173-179.
  • Rattanachaikunsopon, P., Phumkhachorn, P. (2010). Lactic acid bacteria: their antimicrobial compounds and their uses in food production, Annals of Biological Research, 1,218-228.
  • Tenreiro, S., Nunes, PcA., Viegas, C.A., et al. (2002). AQR1 gene (ORF YNL065w) encodes a plasma membrane transporter of the major facilitator superfamily that confers resistance to short-chain monocarboxylic acids and quinidine in Saccharomyces cerevisiae, Biochemical and Biophysical Research Communications, 292(3),741-748.
  • Ullah, A., Orij, R., Brul, S., Smits, G.J. (2012). Quantitative analysis of the modes of growth inhibition by weak organic acids in Saccharomyces cerevisiae, Applied and Environmental Microbiology, 78(23), 8377-8387.
  • Viegas, C.A., Rosa, M.F., Sá-Correia, I., Novais, J.M. (1989). Inhibition of yeast growth by octanoic and decanoic acids produced during ethanolic fermentation, Applied and Environmental Microbiology, 55(1),21-2.
  • Viegas, C.A., Sá-Correia, I. (1997). Effects of low temperatures (9–33 C) and pH (3.3–5.7) in the loss of Saccharomyces cerevisiae viability by combining lethal concentrations of ethanol with octanoic and decanoic acids, International Journal of Food Microbiology, 34(3),267-277.
  • Wang, Y., Zeng, X., Zhou, Z., et al. (2015). Inhibitory effect of nerol against Aspergillus niger on grapes through a membrane lesion mechanism, Food Control, 55:54-61.
There are 21 citations in total.

Details

Primary Language Turkish
Subjects Structural Biology
Journal Section Research Articles
Authors

Hatice Büşra Konuk 0000-0002-7115-7211

Bengü Ergüden

Publication Date December 31, 2018
Submission Date August 7, 2018
Acceptance Date December 3, 2018
Published in Issue Year 2018 Volume: 3 Issue: 3

Cite

APA Konuk, H. B., & Ergüden, B. (2018). Çeşitli zayıf organik asitler ve kombinasyonlarının saccharomyces cerevisiae’ye karşı antifungal etkileri. Journal of Advances in VetBio Science and Techniques, 3(3), 28-34. https://doi.org/10.31797/vetbio.451505

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