Isolation of some gram-negative bacterial species from naturally infected silver European eel fish (Anguilla anguilla linnaeus, 1758)

Year 2019, Volume: 4 Issue: 1, 9 - 15, 30.04.2019

Jale Korun , Yağmur Saadet Çelik , Mehmet Gökoğlu , Mesut Yılmaz

https://doi.org/10.31797/vetbio.544847

Cited By: 1

Abstract

The
aim of this study is to inform isolations of Shewanella putrefaciens, Aeromonas
sobria and A. veronii from the silver European eel
fish (Anguilla anguilla) caught from the Gulf of Antalya, Turkey. Three silver eel
fish samples as freshly dead (mean weight 75 g) were obtained by a local
fisherman. For bacteriological study, inoculations from the head kidney, spleen
and liver were made and inoculated onto Brain Hearth Infusion Agar (BHIA-S)
supplemented with 1.5 % NaCl. The inoculated petri dishes were incubated at 24
± 2°C for 72 hours. The end of the incubation period, bacterial colonies were
subcultured. For presumptive identification of the bacterial strains, the
strains were identified using a variety of phenotypic characteristics. The
further identification of the strains was performed with PCR. Clinically, the
silver eel samples showed pale gills, losses of scale, necrotic lesions on
dorsal, hemorrhagies on the jaws, lateral and ventral sides of the body and
around the anus and hemoragic anal fin. At necropsy, the fish had enlarged and
pale spleen and the gastrointestinal tract was empty. After 72 hours, 10 bacterial
strains were isolated. 6 strains as Shewanella
putrefaciens, 2 strains as Aeromonas
sobria and 2 strains as A. veronii were identified according to the
phenotypical characteristics and PCR study results. The eel fish stocks in
natural conditions are decreasing and this problem may be multifactorial such
as disease, migration and overhunting. As a result of this study, A. veronii
was first isolated from A. anguilla.

Keywords

A. anguilla, Shewanella putrefaciens, Aeromonas sobria, A. veronii

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