Western Blot

Year 2021, Volume: 6 Issue: 1, 45 - 56, 30.04.2021

Hatice Pelin Aslım Oya Bulut

https://doi.org/10.31797/vetbio.799660

Abstract

The Western Blot technique is an old and reliable method used in molecular biology to uniquely detect a single protein from any sample. It is a frequently used method for investigating the responses of proteins to stimuli, evaluating protein expression as either absent or increased-decreased, determining cell specific protein isoforms and determining proteins responsible for the occurrence of genetic diseases. In this composition, , information has been provided about principle, protocol and use of Western Blot technique in virology.

Keywords

Method, Virology, Western Blot

Project Number

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Western Blot

Abstract

Western Blot tekniği, herhangi bir örnekten istenen tek bir proteini özgün bir şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir bir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik protein izoformlarının belirlenmesinde, genetik hastalıkların ortaya çıkışından sorumlu proteinlerin belirlenmesinde sıklıkla kullanılan bir metottur. Yapılan bu derlemede, Western Blot tekniğinin prensibi, protokolü ve virolojide kullanımı hakkında bilgi verilmiştir.

Keywords

Metot, Violoji, Western Blot

Supporting Institution

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Project Number

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Thanks

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