Koyunlarda kan BHBA seviyesinin fertilite üzerine etkisi

Year 2022, Volume: 7 Issue: 2, 220 - 227, 31.08.2022

Şükrü Güngör Haydar Özkara Muhammed İnanç Mine Herdoğan Feyzanur Mart Hasan Ali Çay Ramazan Yıldız Hidir Gümüş Ahmet Cumhur Akın Ayhan Ata

https://doi.org/10.31797/vetbio.1117222

Abstract

Bu çalışmada koyunlarda sezon dışı dönemde senkronizasyon öncesi kan BHBA seviyesinin gebelik oranlarına üzerine etkisinin incelenmesi amaçlandı. Bu amaçla, Burdur ilinde bulunan en az 1 doğum yapmış 2-4 yaşlı 100 baş Merinos/Merinos melezi koyun kullanıldı. Senkronizasyon amacıyla hayvanlara progesteron emdirilmiş süngerler 60 mg Medroxy progesteron acetat (MPA) intravaginal olarak 14 gün süre ile uygulandı. Uygulama günü hayvanlardan kan alınarak, BHBA (betahidroksibütirik asit) ölçümü yapıldı. Süngerler çıkartılırken, 500-700 IU aralığında kas içi PMSG uygulaması yapıldı. Süngerlerin çıkarılmasından 24 saat sonra arama koçu yardımı ile östruslar gözlendi ve östrusta olduğu belirlenen hayvanlar elde sıfat yöntemi ile çiftleştirildi. Çiftleştirmeleri takiben 35-45. gün aralığında transrektal prob kullanılarak ultrason yardımı ile gebelikler kaydedildi. Senkronizasyona alınan hayvanların tamamının östrusta olduğu belirlendi ve aşımları gerçekleştirildi. Yapılan gebelik muayenesi sonucunda hayvanların 57 baş hayvan gebe (%57) iken 43 baş (%43) hayvan gebe kalmamıştır. Çalışmada, kan BHBA değerleri 0,12 mmol/L -0,66 mmol/L (n:100) aralığında ölçülmüş olup ortalama 0,35±0,083 mmol/L olarak tespit edilmiştir. Gebe kalan hayvanların kan BHBA seviyesi 0,29±0,005 mmol/L olarak tespit edilirken, gebe kalmayan koyunların kan BHBA seviyesi 0,41±0,073 mmol/L olarak tespit edilmiştir. (p<0,001). Ayrıca çalışmada kullanılan koyunlarda kan BHBA düzeyi ile gebelik oranları arasında güçlü bir negatif korelasyon olduğu belirlenmiştir (r = −0,719, p<0,001). Sonuç olarak literatürde anılan dönemde koyunların fertilite parametreleri ile kan BHBA düzeyi ile arasındaki ilişki hakkında bir çalışma olmaması sunulan çalışmanın literatüre önemli bir katkı sağladığı kanısına varılmıştır. Düşük BHBA seviyelerine sahip olan hayvanlardan daha yüksek bir fertilite başarısı elde edilebileceği, yüksek BHBA seviyesine sahip hayvanların enerji dengelerinin düzenlenerek fertilite oranlarının yükseltilebileceği, yapılan çalışmanın küçükbaş hayvanlarda sürü yönetimi açısından 2 yılda 3 kuzulatma hedefinin sağlanmasında dikkat edilmesi gereken bir kriter olabileceği sonucuna varılmıştır.

Keywords

BHBA, Fertilite, Koyun, Senkronizasyon.

Supporting Institution

TÜBİTAK

Project Number

2209/A 2020-2 1919B012003009

The effect of blood BHBA level on fertility in sheep

Abstract

The aim of this study was the effect of pre-synchronization blood BHBA level on pregnancy rates of sheep in the non-breeding season. For this purpose, 100 Merino/Merino crossbred ewe, aged 2-4 years, who have given one birth before, were used in the study. For synchronization purposes, progesterone containing sponges 60 mg Medroxyprogesteron acetate (MPA) analogue, were administered intravaginally for 14 days. BHBA (betahydroxybutyric acid) measurement was made by drawing blood in each animal on the day of administration. 500-700 IU PMSG was injected at the time of sponge withdrawal. Oestrus was observed 24 hours after sponge withdrawal with the help of teaser ram. Animals which was in estrus were hand mated. Pregnancy status was recorded with the help of ultrasound using the transrectal probe in the interval 35-45 days after mating.
All of the synchronized animals were determined to be in estrus and their mating were performed. As a result of the pregnancy examination, 57 (57%) of the animals became pregnant and 43 (43%) did not become pregnant. Blood BHBA values were measured in the range of 0.12 mmol/L -0.66 mmol/L (n:100) in present study and the average of BHBA as 0.35±0.083 mmol/L was determined. While blood BHBA level of pregnant animals was found as 0.29±0.005 mmol/L, blood BHBA level of non-pregnant sheep was found to be 0.41±0.073 mmol/L, which was statistically significant (p<0.001). In addition, it was determined that there was a strong negative correlation between blood BHBA level and pregnancy rates in sheep (r = −0.719, p<0.001).
As a result, it was concluded that there was no study in the literature about the relationship between the fertility parameters of sheep and the blood BHBA level in the mentioned period, and it was concluded that the presented study made a significant contribution to the literature. It has been concluded that a higher fertility success can be achieved from animals with low BHBA levels, that fertility rates can be increased by regulating the energy balance of animals with high BHBA levels, and that the study can be a criterion to be considered in achieving the target of 3 lambing in 2 years in terms of herd management in sheep breeding.

Keywords

BHBA, Fertility, Sheep, Synchronization

Project Number

2209/A 2020-2 1919B012003009

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