Research Article
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Expression of IL-8 Gene in Rainbow Trout (Oncorhynchus mykiss) Leucocytes Fed with Uryani plum (Prunus domestica) Extract

Year 2024, , 1 - 8, 30.06.2024
https://doi.org/10.61262/vetjku.1481672

Abstract

Aim to study: In the present study, interleukin-8 (IL-8) gene expression was determined in rainbow trout (Oncorhynchus mykiss) after treatment with uryani plum (Prunus domestica) extract.
Material and methods: Fish leucocyte was stimulated with plum extract at the rate of 0 (control), 5 mg/ml, 10 mg/ml and 15 mg/ml. Cells were collected at 1, 4, 12, 24, and 48 hours of the study and IL-8 gene expression was determined in each group.
Results: The result showed an increase in IL-8 expression in all experimental groups compared to the control at the end of the study.
Conclusion: The results suggested that uryani plum (Prunus domestica) extract could activate immune responses of rainbow trout head kidney leukocytes in rainbow trout.

Ethical Statement

This study was approved by the Kastamonu University Animal Experiments Local Ethics Committee (KUHADYEK-14.12.2020-2020.35).

References

  • Abarca, A., Bethke, J., Narváez, E., Flores, R., & Mercado, L. (2012). Parameters to evaluate the immunostimulant effect of Zymosan A in head kidney leucocytes (HKL) of salmonids. Latin american journal of aquatic research, 40(3), 545-552. https://doi.org/103856/vol40-issue3-fulltext-4.
  • Ali, G. A. M., Bilen, S., & Güney, K. (2022). Growth promoter, immunostimulant and antioxidant for rainbow trout (Oncorhynchus mykiss): Terebinth (Pistacia terebinthus) extract. Marine Science and Technology Bulletin, 11(1), 98-112. https://doi.org/10.33714/masteb.1063278.
  • Almabrok, A. A., Amhamed, I. D., Mohamed, G. A., Bilen, S., & Altief, T. A. S. (2018). Effect of Tilia tomentosa methanolic extract on growth performance, digestive enzyme activity, immune response and haematological indices of common carp (Cyprinus carpio). Marine Science and Technology Bulletin, 7(1), 12-20. https://doi.org/10.33714/masteb.421047.
  • Altunoglu, Y. C., Bilen, S., Ulu, F., & Biswas, G. (2017). Immune responses to methanolic extract of black cumin (Nigella sativa) in rainbow trout (Oncorhynchus mykiss). Fish & shellfish immunology, 67, 103-109. https://doi.org/10.1016/j.fsi.2017.06.002.
  • Awad, E., Mitchell, W. J., & Austin, B. (2011). Effect of dietary supplements on cytokine gene expression in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of fish diseases, 34(8). https://doi.org/10.1111/j.1365-2761.2011.01271.x.
  • Bilen, S., Ali, G. A. M., Amhamed, I. D., & Almabrok, A. A. (2021b). Modulatory effects of laurel-leaf cistus (Cistus laurifolius) ethanolic extract on innate immune responses and disease resistance in common carp (Cyprinus carpio). Fish & Shellfish Immunology, 116, 98-106. https://doi.org/10.1016/j.fsi.2021.07.001.
  • Bilen, S., Filogh, A. M., Ali, A. B., Kenanoğlu, O. N., & Zoral, M. A. (2020). Effect of common mallow (Malva sylvestris) dietary supplementation on growth performance, digestive enzyme activities, haemotological and immune responses of common carp (Cyprinus carpio). Aquaculture International, 28, 73-84. https://doi.org/10.1007/s10499-019-00444-9.
  • Bilen, S., Ispir, S., Kenanoglu, O. N., Taştan, Y., Güney, K., & Terzi, E. (2021a). Effects of Greek juniper (Juniperus excelsa) extract on immune responses and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Journal of Fish Diseases, 44(6), 729-738. https://doi.org/10.1111/jfd.13293.
  • Bilen, S., Kızak, V., & Bilen, A. M. (2013). Floating fish farm unit (3FU). Is it an appropriate method for Salmonid production? Marine Science and Technology Bulletin, 2(1), 9–13. https://doi.org/10.1016/j.fsi.2021.07.001
  • Bilen, S., Ünal, S., & Güvensoy, H. (2016). Effects of oyster mushroom (Pleurotus ostreatus) and nettle (Urtica dioica) methanolic extracts on immune responses and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss). Aquaculture, 454,90-94. https://doi.org/10.1016/j.aquaculture.2015.12.010.
  • Elbesthi, R. T. A., Özdemir, K. Y., Taştan, Y., Bilen, S., & Sönmez, A. Y. (2020). Effects of ribwort plantain (Plantago lanceolata) extract on blood parameters, immune response, antioxidant enzyme activities, and growth performance in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 46, 1295-1307. https://doi.org/10.1007/s10695-020-00790-z.
  • FAO (2022). The state of world fisheries and aquaculture 2022. Towards Blue Transformation. Rome, FAO. https://doi.org/10.4060/cc0461en.
  • Fierro-Castro, C., Barrioluengo, L., López-Fierro, P., Razquin, B. E., Carracedo, B., & Villena, A. J. (2012). Fish cell cultures as in vitro models of pro-inflammatory responses elicited by immunostimulants. Fish & shellfish immunology, 33(2), 389-400. https://doi.org/10.1016/j.fsi.2012.05.019.
  • Galeotti, M. (1998). Some aspects of the application of immunostimulants and a critical review of methods for their evaluation. Journal of Applied Ichthyology, 14(3‐4), 89-199. https://doi.org/10.1111/j.1439-0426.1998.tb0064 1.x.
  • Hooshmand, S., Kumar, A., Zhang, J. Y., Johnson, S. A., Chai, S. C., & Arjmandi, B. H. (2015). Evidence for anti-inflammatory and antioxidative properties of dried plum polyphenols in macrophage RAW 264.7 cells. Food & function, 6(5), 1719-1725. https://doi.org/10.1039/C5FO00173K.
  • Jimenez, N., Coll, J., Salguero, F. J., & Tafalla, C. (2006). Co-injection of interleukin 8 with the glycoprotein gene from viral haemorrhagic septicemia virus (VHSV) modulates the cytokine response in rainbow trout (Oncorhynchus mykiss). Vaccine, 24(27-28), 5615-5626. https://doi.org/10.1016/j.vaccine.2006.04.061.
  • Kendrick, A. A., Holliday, M. J., Isern, N. G., Zhang, F., Camilloni, C., Huynh, C., Vendruscolo, M., Armstrong, G. & Eisenmesser, E. Z. (2014). The dynamics of interleukin‐8 and its interaction with human CXC receptor I peptide. Protein Science, 23(4), 464-480. https://doi.org/10.1002/pro.2430.
  • Lakwani, M. A., Kenanoğlu, O. N., Taştan, Y., & Bilen, S. (2021). Effects of black mustard (Brassica nigra) seed oil on growth performance, digestive enzyme activities and immune responses in rainbow trout (Oncorhynchus mykiss). Aquaculture Research, 53, 300–313. https://doi.org/10.1111/are.15577.
  • Midtlyng, P. J., Hendriksen, C., Balks, E., Bruckner, L., Elsken, L., Evensen, Ø., Fyrand, K., Guy, A., Halder, M., Hawkins, P., Kisen, G., Romstad, A. B., Salonius, K., Smith, P. & Sneddon, L. U. (2011). Three Rs approaches in the production and quality control of fish vaccines. Biologicals, 39(2), 117-128. https://doi.org/10.1016/j.biologicals.2011.02.001.
  • Mulero, V., Esteban, M. A., Munoz, J., & Meseguer, J. (1998). Dietary intake of levamisole enhances the immune response and disease resistance of the marine teleost gilthead seabream (Sparus aurata L.). Fish & Shellfish Immunology, 8(1), 49-62. https://doi.org/10.1006/fsim.1997.0119.
  • Picchietti, S., Bernini, C., Belardinelli, M. C., Ovidi, E., Taddei, A. R., Guerra, L., Abelli, L., Fausto, A. M. (2013). Immune modulatory effects of Aloe arborescens extract on the piscine SAF-1 cell line. Fish & shellfish immunology, 34(5), 1335-1344. https://doi.org/10.1016/j.fsi.2013.02.019.
  • Reverter, M., Sarter, S., Caruso, D., Avarre, J. C., Combe, M., Pepey, E., Pouyaud, L., Vega-Heredia, S., Gozlan, R. E. (2020). Aquaculture at the crossroads of global warming and antimicrobial resistance. Nature communications, 11(1), 1870. https://doi.org/10.1038/s41467-020-15735-6
  • Salem, M. O. A., Taştan, Y., Bilen, S., Terzi, E., & Sönmez, A. Y. (2022). Effects of white mustard (Sinapis alba) oil on growth performance, immune response, blood parameters, digestive and antioxidant enzyme activities in rainbow trout (Oncorhynchus mykiss). Fish & shellfish immunology, 131, 283-299. https://doi.org/10.1016/j.fsi.2022.10.006.
  • Schmittgen, T. D., & Livak, K. J. (2008). Analyzing real-time PCR data by the comparative CT method. Nature protocols, 3(6), 1101-1108. https://doi.org/10.1038/nprot.2008.73.
  • Schnell, S., Kawano, A., Porte, C., Lee, L. E., & Bols, N. C. (2009). Effects of ibuprofen on the viability and proliferation of rainbow trout liver cell lines and potential problems and interactions in effects assessment. Environmental Toxicology: An International Journal, 24(2), 157-165. https://doi.org/10.1002/tox.20410.
  • Sigh, J., Lindenstrøm, T., & Buchmann, K. (2004). Expression of pro-inflammatory cytokines in rainbow trout (Oncorhynchus mykiss) during an infection with Ichthyophthirius multifiliis. Fish & shellfish immunology, 17(1), 75-86. https://doi.org/10.1016/j.fsi.2003.12.005.
  • Sönmez, A. Y., Bilen, S., Taştan, Y., Serag, K. J. B., Toring, C. C., Romero, J. B., Kenanoğlu, O. N., & Terzi, E. (2021). Oral administration of Sargassum polycystum extracts stimulates immune response and increases survival against Aeromonas hydrophila infection in Oncorhynchus mykiss. Fish & Shellfish Immunology, 117, 291-298. https://doi.org/10.1016/j.fsi.2021.08.020.
  • Terzi, E., Kucukkosker, B., Bilen, S., Kenanoglu, O. N., Corum, O., Özbek, M., & Parug, S. S. (2021). A novel herbal immunostimulant for rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Fish & Shellfish Immunology, 110, 55-66. https://doi.org/10.1016/j.fsi.2020.12.019.
  • Thanigaivel, S., Chandrasekaran, N., Mukherjee, A., & Thomas, J. (2016). Seaweeds as an alternative therapeutic source for aquatic disease management. Aquaculture, 464, 529-536. https://doi.org/10.1016/j.aquaculture.2016.08.001.
  • Wang, G. L., Wang, M. C., Zhang, X. W., Chang, M. X., Xie, H. X., & Nie, P. (2017). Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection. Fish & Shellfish Immunology, 66, 112-119. https://doi.org/10.1016/j.fsi.2017.05.016.
  • Yin, G., Jeney, G., Racz, T., Xu, P., Jun, X., & Jeney, Z. (2006). Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus. Aquaculture, 253(1-4), 39-47. https://doi.org/10.1016/j.aquaculture.2005.06.038.
  • Zanuzzo, F. S., Biller-Takahashi, J. D., & Urbinati, E. C. (2012). Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxa during the transport stress. Revista Brasileira de Zootecnia, 41, 2299-2302.https://doi.org/10.1590/S1516-35982012010 00023.
Year 2024, , 1 - 8, 30.06.2024
https://doi.org/10.61262/vetjku.1481672

Abstract

References

  • Abarca, A., Bethke, J., Narváez, E., Flores, R., & Mercado, L. (2012). Parameters to evaluate the immunostimulant effect of Zymosan A in head kidney leucocytes (HKL) of salmonids. Latin american journal of aquatic research, 40(3), 545-552. https://doi.org/103856/vol40-issue3-fulltext-4.
  • Ali, G. A. M., Bilen, S., & Güney, K. (2022). Growth promoter, immunostimulant and antioxidant for rainbow trout (Oncorhynchus mykiss): Terebinth (Pistacia terebinthus) extract. Marine Science and Technology Bulletin, 11(1), 98-112. https://doi.org/10.33714/masteb.1063278.
  • Almabrok, A. A., Amhamed, I. D., Mohamed, G. A., Bilen, S., & Altief, T. A. S. (2018). Effect of Tilia tomentosa methanolic extract on growth performance, digestive enzyme activity, immune response and haematological indices of common carp (Cyprinus carpio). Marine Science and Technology Bulletin, 7(1), 12-20. https://doi.org/10.33714/masteb.421047.
  • Altunoglu, Y. C., Bilen, S., Ulu, F., & Biswas, G. (2017). Immune responses to methanolic extract of black cumin (Nigella sativa) in rainbow trout (Oncorhynchus mykiss). Fish & shellfish immunology, 67, 103-109. https://doi.org/10.1016/j.fsi.2017.06.002.
  • Awad, E., Mitchell, W. J., & Austin, B. (2011). Effect of dietary supplements on cytokine gene expression in rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of fish diseases, 34(8). https://doi.org/10.1111/j.1365-2761.2011.01271.x.
  • Bilen, S., Ali, G. A. M., Amhamed, I. D., & Almabrok, A. A. (2021b). Modulatory effects of laurel-leaf cistus (Cistus laurifolius) ethanolic extract on innate immune responses and disease resistance in common carp (Cyprinus carpio). Fish & Shellfish Immunology, 116, 98-106. https://doi.org/10.1016/j.fsi.2021.07.001.
  • Bilen, S., Filogh, A. M., Ali, A. B., Kenanoğlu, O. N., & Zoral, M. A. (2020). Effect of common mallow (Malva sylvestris) dietary supplementation on growth performance, digestive enzyme activities, haemotological and immune responses of common carp (Cyprinus carpio). Aquaculture International, 28, 73-84. https://doi.org/10.1007/s10499-019-00444-9.
  • Bilen, S., Ispir, S., Kenanoglu, O. N., Taştan, Y., Güney, K., & Terzi, E. (2021a). Effects of Greek juniper (Juniperus excelsa) extract on immune responses and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Journal of Fish Diseases, 44(6), 729-738. https://doi.org/10.1111/jfd.13293.
  • Bilen, S., Kızak, V., & Bilen, A. M. (2013). Floating fish farm unit (3FU). Is it an appropriate method for Salmonid production? Marine Science and Technology Bulletin, 2(1), 9–13. https://doi.org/10.1016/j.fsi.2021.07.001
  • Bilen, S., Ünal, S., & Güvensoy, H. (2016). Effects of oyster mushroom (Pleurotus ostreatus) and nettle (Urtica dioica) methanolic extracts on immune responses and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss). Aquaculture, 454,90-94. https://doi.org/10.1016/j.aquaculture.2015.12.010.
  • Elbesthi, R. T. A., Özdemir, K. Y., Taştan, Y., Bilen, S., & Sönmez, A. Y. (2020). Effects of ribwort plantain (Plantago lanceolata) extract on blood parameters, immune response, antioxidant enzyme activities, and growth performance in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 46, 1295-1307. https://doi.org/10.1007/s10695-020-00790-z.
  • FAO (2022). The state of world fisheries and aquaculture 2022. Towards Blue Transformation. Rome, FAO. https://doi.org/10.4060/cc0461en.
  • Fierro-Castro, C., Barrioluengo, L., López-Fierro, P., Razquin, B. E., Carracedo, B., & Villena, A. J. (2012). Fish cell cultures as in vitro models of pro-inflammatory responses elicited by immunostimulants. Fish & shellfish immunology, 33(2), 389-400. https://doi.org/10.1016/j.fsi.2012.05.019.
  • Galeotti, M. (1998). Some aspects of the application of immunostimulants and a critical review of methods for their evaluation. Journal of Applied Ichthyology, 14(3‐4), 89-199. https://doi.org/10.1111/j.1439-0426.1998.tb0064 1.x.
  • Hooshmand, S., Kumar, A., Zhang, J. Y., Johnson, S. A., Chai, S. C., & Arjmandi, B. H. (2015). Evidence for anti-inflammatory and antioxidative properties of dried plum polyphenols in macrophage RAW 264.7 cells. Food & function, 6(5), 1719-1725. https://doi.org/10.1039/C5FO00173K.
  • Jimenez, N., Coll, J., Salguero, F. J., & Tafalla, C. (2006). Co-injection of interleukin 8 with the glycoprotein gene from viral haemorrhagic septicemia virus (VHSV) modulates the cytokine response in rainbow trout (Oncorhynchus mykiss). Vaccine, 24(27-28), 5615-5626. https://doi.org/10.1016/j.vaccine.2006.04.061.
  • Kendrick, A. A., Holliday, M. J., Isern, N. G., Zhang, F., Camilloni, C., Huynh, C., Vendruscolo, M., Armstrong, G. & Eisenmesser, E. Z. (2014). The dynamics of interleukin‐8 and its interaction with human CXC receptor I peptide. Protein Science, 23(4), 464-480. https://doi.org/10.1002/pro.2430.
  • Lakwani, M. A., Kenanoğlu, O. N., Taştan, Y., & Bilen, S. (2021). Effects of black mustard (Brassica nigra) seed oil on growth performance, digestive enzyme activities and immune responses in rainbow trout (Oncorhynchus mykiss). Aquaculture Research, 53, 300–313. https://doi.org/10.1111/are.15577.
  • Midtlyng, P. J., Hendriksen, C., Balks, E., Bruckner, L., Elsken, L., Evensen, Ø., Fyrand, K., Guy, A., Halder, M., Hawkins, P., Kisen, G., Romstad, A. B., Salonius, K., Smith, P. & Sneddon, L. U. (2011). Three Rs approaches in the production and quality control of fish vaccines. Biologicals, 39(2), 117-128. https://doi.org/10.1016/j.biologicals.2011.02.001.
  • Mulero, V., Esteban, M. A., Munoz, J., & Meseguer, J. (1998). Dietary intake of levamisole enhances the immune response and disease resistance of the marine teleost gilthead seabream (Sparus aurata L.). Fish & Shellfish Immunology, 8(1), 49-62. https://doi.org/10.1006/fsim.1997.0119.
  • Picchietti, S., Bernini, C., Belardinelli, M. C., Ovidi, E., Taddei, A. R., Guerra, L., Abelli, L., Fausto, A. M. (2013). Immune modulatory effects of Aloe arborescens extract on the piscine SAF-1 cell line. Fish & shellfish immunology, 34(5), 1335-1344. https://doi.org/10.1016/j.fsi.2013.02.019.
  • Reverter, M., Sarter, S., Caruso, D., Avarre, J. C., Combe, M., Pepey, E., Pouyaud, L., Vega-Heredia, S., Gozlan, R. E. (2020). Aquaculture at the crossroads of global warming and antimicrobial resistance. Nature communications, 11(1), 1870. https://doi.org/10.1038/s41467-020-15735-6
  • Salem, M. O. A., Taştan, Y., Bilen, S., Terzi, E., & Sönmez, A. Y. (2022). Effects of white mustard (Sinapis alba) oil on growth performance, immune response, blood parameters, digestive and antioxidant enzyme activities in rainbow trout (Oncorhynchus mykiss). Fish & shellfish immunology, 131, 283-299. https://doi.org/10.1016/j.fsi.2022.10.006.
  • Schmittgen, T. D., & Livak, K. J. (2008). Analyzing real-time PCR data by the comparative CT method. Nature protocols, 3(6), 1101-1108. https://doi.org/10.1038/nprot.2008.73.
  • Schnell, S., Kawano, A., Porte, C., Lee, L. E., & Bols, N. C. (2009). Effects of ibuprofen on the viability and proliferation of rainbow trout liver cell lines and potential problems and interactions in effects assessment. Environmental Toxicology: An International Journal, 24(2), 157-165. https://doi.org/10.1002/tox.20410.
  • Sigh, J., Lindenstrøm, T., & Buchmann, K. (2004). Expression of pro-inflammatory cytokines in rainbow trout (Oncorhynchus mykiss) during an infection with Ichthyophthirius multifiliis. Fish & shellfish immunology, 17(1), 75-86. https://doi.org/10.1016/j.fsi.2003.12.005.
  • Sönmez, A. Y., Bilen, S., Taştan, Y., Serag, K. J. B., Toring, C. C., Romero, J. B., Kenanoğlu, O. N., & Terzi, E. (2021). Oral administration of Sargassum polycystum extracts stimulates immune response and increases survival against Aeromonas hydrophila infection in Oncorhynchus mykiss. Fish & Shellfish Immunology, 117, 291-298. https://doi.org/10.1016/j.fsi.2021.08.020.
  • Terzi, E., Kucukkosker, B., Bilen, S., Kenanoglu, O. N., Corum, O., Özbek, M., & Parug, S. S. (2021). A novel herbal immunostimulant for rainbow trout (Oncorhynchus mykiss) against Yersinia ruckeri. Fish & Shellfish Immunology, 110, 55-66. https://doi.org/10.1016/j.fsi.2020.12.019.
  • Thanigaivel, S., Chandrasekaran, N., Mukherjee, A., & Thomas, J. (2016). Seaweeds as an alternative therapeutic source for aquatic disease management. Aquaculture, 464, 529-536. https://doi.org/10.1016/j.aquaculture.2016.08.001.
  • Wang, G. L., Wang, M. C., Zhang, X. W., Chang, M. X., Xie, H. X., & Nie, P. (2017). Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection. Fish & Shellfish Immunology, 66, 112-119. https://doi.org/10.1016/j.fsi.2017.05.016.
  • Yin, G., Jeney, G., Racz, T., Xu, P., Jun, X., & Jeney, Z. (2006). Effect of two Chinese herbs (Astragalus radix and Scutellaria radix) on non-specific immune response of tilapia, Oreochromis niloticus. Aquaculture, 253(1-4), 39-47. https://doi.org/10.1016/j.aquaculture.2005.06.038.
  • Zanuzzo, F. S., Biller-Takahashi, J. D., & Urbinati, E. C. (2012). Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxa during the transport stress. Revista Brasileira de Zootecnia, 41, 2299-2302.https://doi.org/10.1590/S1516-35982012010 00023.
There are 32 citations in total.

Details

Primary Language English
Subjects Veterinary Sciences (Other)
Journal Section Research Articles
Authors

Nazlı Ceren Akman This is me 0009-0009-5271-6346

Elif Melisa Yilmaz This is me 0009-0004-0254-5845

Kaan Karaali This is me 0009-0007-7218-0337

Soner Bilen 0000-0001-9459-8178

Publication Date June 30, 2024
Submission Date May 11, 2024
Acceptance Date June 27, 2024
Published in Issue Year 2024

Cite

APA Akman, N. C., Yilmaz, E. M., Karaali, K., Bilen, S. (2024). Expression of IL-8 Gene in Rainbow Trout (Oncorhynchus mykiss) Leucocytes Fed with Uryani plum (Prunus domestica) Extract. Veterinary Journal of Kastamonu University, 3(1), 1-8. https://doi.org/10.61262/vetjku.1481672