Effects of Apilarnil on Semen Parameters During Ram Semen Short-Term Storage

Abstract

This study aimed to investigate the effects of different concentrations of apilarnil (APL) added to semen diluent on the short-term storage of ram semen. For this purpose, six Akkaraman rams aged 1.5 years were used in the study outside the breeding season. Semen was collected from the rams twice a week with the help of an artificial vagina. Semen was first analyzed and ejaculates with a total motility score of 70% and above were pooled. The pooled ejaculates were divided into 5 equal parts and reconstituted with tris-egg yolk diluent containing 0%, 0.5%, 1%, 1%.5% and 2% APL. Diluted semen samples were stored at 4oC for 72 hours. As a result of the analyses, no statistically significant difference was observed between the groups in terms of total motility at 0 hour, while progressive motility was significantly higher in the 0.5% APL group (P < .005). While rapid spermatozoa rate, curvilinear velocity (VCL), and linear velocity (VSL) were high in the control group in all time periods, medium, and slow spermatozoa rates were high in 1% and 1.5% APL groups (P < .05). At 0 and 72 hours, the control group had the highest malondialdehyde (MDA) level (P < .001). Glutathione (GSH) level and glutathione peroxidase (GSH-Px) and catalase (CAT) activities were significantly lower in the control group at 0 and 72 hours. In conclusion, 0.5-1.5% APL added to semen diluent for short-term storage of ram semen has a positive effect on semen quality and oxidant status.

Keywords

• apilarnil
• CASA
• cooling
• ram
• sperm

Koç Spermasının Kısa Süreli Saklanması Sırasında Apilarnil'in Sperma Parametreleri Üzerindeki Etkileri

Abstract

Bu çalışma, sperma sulandırıcısına eklenen farklı konsantrasyonlardaki apilarnilin (APL) koç spermasının kısa süreli saklanması üzerindeki etkilerini araştırmayı amaçlamıştır. Bu amaçla, çalışmada üreme mevsimi dışında 1,5 yaşında altı Akkaraman koçu kullanıldı. Koçlardan haftada iki kez suni vajina yardımıyla sperma toplandı. Sperma önce analiz edildi ve toplam motilite skoru %70 ve üzerinde olan ejakülatlar pooling yapıldı. Pooling yapılan ejakülatlar 5 eşit parçaya bölündü ve içerisine %0, %0,5, %1, %1,5 ve %2 oranında APL ilave edilmiş tris-yumurta sarısı sulandırıcısı ile sulandırıldı. Seyreltilmiş sperma örnekleri 4oC'de 72 saat boyunca saklandı. Analizler sonucunda, 0. saatte total motilite açısından gruplar arasında istatistiksel olarak anlamlı bir fark gözlenmezken, progresif motilite %0,5 APL grubunda anlamlı olarak daha yüksek bulunmuştur (P < ,005). Hızlı spermatozoa oranı, eğrisel hız (VCL) ve doğrusal hız (VSL) tüm zaman dilimlerinde kontrol grubunda yüksekken, orta ve yavaş spermatozoa oranları %1 ve %1,5 APL gruplarında yüksekti (P < ,05). 0 ve 72. saatlerde kontrol grubu en yüksek malondialdehit (MDA) seviyesine sahipti (P < ,001). Glutatyon (GSH) seviyesi ile glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) aktiviteleri 0 ve 72. saatlerde kontrol grubunda anlamlı derecede düşüktü. Sonuç olarak, koç spermasının kısa süreli saklanmasında sperma sulandırıcısına eklenen %0,5-1,5 APL, sperma kalitesi ve oksidan durumu üzerinde olumlu bir etkiye sahiptir.

Keywords

• Apilarnil
• CASA
• koç
• soğutma
• sperm

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